Results 21 to 30 of about 349,214 (297)

Long-range electron transfer in structurally engineered pentaammineruthenium (histidine-62) cytochrome c [PDF]

open access: yes, 1989
In many biological processes, long-range electron transfer (ET) plays a key role. When the three-dimensional structures of proteins are accurately known, use of modified proteins and protein-protein complexes provides an experimental approach to study ET
Bowler, Bruce E.   +4 more
core   +1 more source

The carboxyl terminus in apolipoprotein E2 and the seven amino acid repeat in apolipoprotein E-Leiden: role in receptor-binding activity

open access: yesJournal of Lipid Research, 1998
Both apolipoprotein (apo) E2 and apoE-Leiden (tandem repeat of amino acids 121–127) are associated with type III hyperlipoproteinemia and bind defectively to low density lipoprotein receptors. Removing the carboxyl terminus of both variants (residues 192–
Li-Ming Dong   +4 more
doaj   +1 more source

Frog foams and natural protein surfactants [PDF]

open access: yes, 2017
Foams and surfactants are relatively rare in biology because of their potential to harm cell membranes and other delicate tissues. However, in recent work we have identified and characterized a number of natural surfactant proteins found in the foam ...
Cooper, Alan   +3 more
core   +1 more source

Site-directed mutation of a laccase from Thermus thermophilus: Effect on the activity profile [PDF]

open access: yesArchives of Biological Sciences, 2012
A site-directed mutant R453T of a laccase from Thermus thermophilus HB27 (Tth-laccase) was constructed in order to investigate the effect on laccase catalytic properties. The mutated gene was cloned and overexpressed in Escherichia coli.
Liu Xin, Tian Meng, Liu Kewu
doaj   +1 more source

Strategies for studying permeation at voltage-gated ion channels [PDF]

open access: yes, 1991
Voltage-dependent ion channels are presently thought to consist of several distinct functional regions: (a) activation gates, (b) inactivation gates, and permeation pathways.
Lester, Henry A.
core   +1 more source

In silico Analysis and Site-directed Mutagenesis of Promoters

open access: yesBio-Protocol, 2017
In normal as in cancerous cells, gene expression is tightly regulated by transcription factors, which are responsible for up- or down-regulation of thousands of targets involved in different cell processes. Transcription factors can directly regulate the
Salah Boudjadi, Jean-François Beaulieu
doaj   +1 more source

Improved immobilization of laccase on a glassy carbon electrode by oriented covalent attachment [PDF]

open access: yesArchives of Biological Sciences, 2014
A laccase from Thermus thermophilus HB27 was reported to be potentially useful in the design of a temperature controlled biofuel cell. For enhancing its application in different thermal conditions, we engineered a laccase-oriented immobilized ...
Liu Xin, Liu Kewu
doaj   +1 more source

Identification of a key residue for Oligomerisation and pore-formation of Clostridium perfringens NetB [PDF]

open access: yes, 2014
Necrotic enteritis toxin B (NetB) is a β-pore-forming toxin produced by Clostridium perfringens and has been identified as a key virulence factor in the pathogenesis of avian necrotic enteritis, a disease causing significant economic damage to the ...
Basak, Ajit K.   +6 more
core   +2 more sources

Current status and emerging frontiers in enzyme engineering: An industrial perspective

open access: yesHeliyon
Protein engineering mechanisms can be an efficient approach to enhance the biochemical properties of various biocatalysts. Immobilization of biocatalysts and the introduction of new-to-nature chemical reactivities are also possible through the same ...
Obinna Giles Ndochinwa   +6 more
doaj   +1 more source

Construction of Glycine Oxidase Mutant Libraries by Random Mutagenesis, Site Directed Mutagenesis and DNA Shuffling

open access: yesBio-Protocol, 2014
Glyphosate, a broad spectrum herbicide widely used in agriculture all over the world, inhibits 5-enolpyruvylshikimate-3-phosphate synthase in the shikimate pathway, and glycine oxidase (GO) has been reported to be able to catalyze the oxidative ...
Tao Zhan
doaj   +1 more source

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