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An efficient one-step site-directed deletion, insertion, single and multiple-site plasmid mutagenesis protocol [PDF]

BMC Biotechnology, 2008
Background Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques.
Naismith James H, Liu Huanting
doaj   +2 more sources

A Simple and Economical Site-Directed Mutagenesis Method for Large Plasmids by Direct Transformation of two Overlapping PCR Fragments

BioTechniques, 2022
Despite the development of various methods and commercial kits, site-directed mutagenesis of large plasmids remains a challenge in many laboratories.
Yueping Zhang
exaly   +3 more sources

Site-directed mutagenesis: colony growth [PDF]

BioTechniques, 2011
This month's question from the Molecular Biology Forums (online at molecularbiology. forums.biotechniques.com) comes from the "General Methods" section. Entries have been edited for concision and clarity.
Kristie Nybo
doaj   +3 more sources

Site-Directed Mutagenesis of Large Plasmids

BioTechniques, 1998
A protocol combining recombination PCR and long-distance PCR is demonstrated to be highly accurate and rapid for sitedirected mutagenesis of large (>10 kb) plasmids.
Susan A. Nadin-Davis, Shirley C. Chang
doaj   +3 more sources

Efficient method for site-directed mutagenesis in large plasmids without subcloning. [PDF]

PLoS ONE, 2017
Commonly used methods for site-directed DNA mutagenesis require copying the entire target plasmid. These methods allow relatively easy modification of DNA sequences in small plasmids but become less efficient and faithful for large plasmids ...
Louay K Hallak, Kelly Berger, Rita Kaspar, Anna R Kwilas, Federica Montanaro, Mark E Peeples   +5 more
doaj   +5 more sources

General Method for Site-Directed Mutagenesis [PDF]

BioTechniques, 2001
Reiner Andag, Ekkehard Schütz
doaj   +4 more sources

Editorial: Mutational breeding: from induced mutations to site-directed mutagenesis. [PDF]

Front Plant Sci
Rasmussen SK, Jain SM.
europepmc   +2 more sources

A high-efficiency method for site-directed mutagenesis of large plasmids based on large DNA fragment amplification and recombinational ligation

Scientific Reports, 2021
Site-directed mutagenesis for large plasmids is a difficult task that cannot easily be solved by the conventional methods used in many laboratories. In this study, we developed an effective method for Site-directed Mutagenesis for Large Plasmids (SMLP ...
Kewei Zhang, Xiaomei Yin, Kaituo Shi, Shihua Zhang, Juan Wang, Shasha Zhao, Huan Deng, Cheng Zhang, Zihui Wu, Yuan Li, Xiangyu Zhou, Wensheng Deng   +11 more
doaj   +1 more source

insilico.mutagenesis: a primer selection tool designed for sequence scanning applications used in directed evolution experiments

BioTechniques, 2005
Several primer prediction programs have been developed for a variety of applications. However, none of these tools allows the prediction of a large set of primers for whole gene site-directed mutagenesis experiments using the megaprimer method. We report
Ulrich Krauss, Thorsten Eggert
doaj   +1 more source

VH-RELATED IDIOTOPES DETECTED BY SITE-DIRECTED MUTAGENESIS [PDF]

, 1992
The function of the CD4 cell surface protein as coreceptor on T helper lymphocytes and as receptor for HIV makes this glycoprotein a prime target for an immune intervention with mAb.
Cheng, Y. H., Rieber, Ernst Peter, Riethmüller, Gert, Weiss, Elisabeth H., Weissenhorn, Winfried   +4 more
core   +1 more source