Results 91 to 100 of about 247,239 (299)
Oligonucleotides used for site-directed mutagenesis (forward primer). [PDF]
, 2016 Oligonucleotides used for site-directed mutagenesis (forward primer).
Xiuju Li (700858) +3 more
core +1 more source
Antimicrobial characterization of site-directed mutagenesis of porcine beta defensin 2.
PLoS ONE, 2015 Porcine β defensin 2 (pBD2) is a small, cationic and amphiphilic antimicrobial peptide. It has broad antimicrobial activities against bacteria and plays an important role in host defense.
Xian-xian Huang +3 more
doaj +1 more source
Deep Contrastive Learning for High‐Throughput Prediction of Drug Resistance Mutations from Sequences
Advanced Science, EarlyView.This study presents DeepMutDTA, a deep learning framework aimed at predicting mutation‐induced changes in protein‐drug interactions and prioritizing variants potentially linked to drug resistance. Trained on large‐scale data, it incorporates SimSiam‐MuTF, a label‐aware contrastive fine‐tuning strategy that encourages separation between WT and MT ...
Xiaowen Hu +7 more
wiley +1 more source
Scarless and site-directed mutagenesis in
BMC Biotechnology, 2007 Background A variety of techniques have been described which introduce scarless, site-specific chromosomal mutations. These techniques can be applied to make point mutations or gene deletions as well as insert heterologous DNA into bacterial vectors for ...
Berghman Luc R +7 more
doaj +1 more source
Advanced Science, EarlyView.This study identifies an immunometabolic axis wherein SAM‐driven PRMT1 methylates LDHA, enhancing its activity. The resultant lactate induces STAT3 K709 lactylation, which stabilizes an active conformation to promote STAT3 phosphorylation and IL‐10 expression.
Hui Wang +12 more
wiley +1 more source
List of oligonucleotides used for site-directed mutagenesis. [PDF]
, 2013 List of oligonucleotides used for site-directed mutagenesis.
Stefan Knapp (244328) +6 more
core +1 more source
Redox‐Dependent Chaperoning of GBF1 Condensates Regulates Seed Germination in Arabidopsis
Advanced Science, EarlyView.In dormant seeds (low ROS), GBF1 forms liquid condensates to repress the germination gene CathB3, and the chaperone GIP1 maintains condensate liquidity and repressive activity. Upon imbibition (high ROS), ROS oxidize GIP1 during germination, impairing its chaperone function.
Yunying Wang, Xiaofeng Fang
wiley +1 more source
Primers for PCR, sequencing, and site-directed mutagenesis. [PDF]
, 2013 Primers for PCR, sequencing, and site-directed mutagenesis.
James M. Mason (103110) +3 more
core +1 more source
Advanced Science, EarlyView.Metastasis remains a major challenge in colorectal cancer. Using an in vivo shRNA screening system, this study identifies Homeobox D4 as a key metastasis suppressor. Reduced Homeobox D4 expression is associated with aggressive tumor features. Functional and mechanistic analyses show that it inhibits epithelial‐mesenchymal transition by repressing ...
Zhi‐hua Ye +9 more
wiley +1 more source
PCR primer sets used for site-directed mutagenesis. [PDF]
, 2015 PCR primer sets used for site-directed mutagenesis.
Gen-cheng Gong (750554) +7 more
core +1 more source