Results 61 to 70 of about 421,026 (281)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
, 2009 A systematic analysis is performed for quantum phase transitions in a two-dimensional anisotropic spin 1/2 anti-ferromagnetic XYX model in an external magnetic field.
Bo Li +5 more
core +1 more source
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
Ecology and Evolution, 2023 Quantifying space use and segregation, as well as the extrinsic and intrinsic factors affecting them, is crucial to increase our knowledge of species‐specific movement ecology and to design effective management and conservation measures.
Jon Morant +14 more
doaj +1 more source
Robustness of high-fidelity Rydberg gates with single-site addressability [PDF]
, 2014 Controlled phase (CPHASE) gates can in principle be realized with trapped neutral atoms by making use of the Rydberg blockade. Achieving the ultra-high fidelities required for quantum computation with such Rydberg gates is however compromised by ...
Aytac, Jon M. +4 more
core +3 more sources
Splice-site pairing is an intrinsically high fidelity process [PDF]
Proceedings of the National Academy of Sciences, 2009 The extensive alternative splicing in higher eukaryotes has initiated a debate whether alternative mRNA isoforms are generated by an inaccurate spliceosome or are the consequence of highly degenerate splice sites within the human genome.
Fox-Walsh, Kristi L, Hertel, Klemens J
openaire +4 more sources
Epilepsy‐Associated Variants of a Single SCN1A Codon Exhibit Divergent Functional Properties
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Pathogenic variants in SCN1A, which encodes the voltage‐gated sodium channel NaV1.1, are associated with multiple epilepsy syndromes exhibiting a range of clinical severity. SCN1A variants are reported in different syndromes, including Dravet syndrome, which is associated with loss‐of‐function, whereas neonatal/infantile‐onset ...
Lanie N. Liebovitz +3 more
wiley +1 more source
Quantum Communication Through a Spin-Ring with Twisted Boundary Conditions [PDF]
, 2004 We investigate quantum communication between the sites of a spin-ring with twisted boundary conditions. Such boundary conditions can be achieved by a flux through the ring.
B.-Q. Jin +4 more
core +1 more source
A Systematic Comparison of Alpha‐Synuclein Seed Amplification Assays for Increasing Reproducibility
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Seed amplification assays (SAAs) enable ultrasensitive detection of misfolded α‐synuclein across biofluids and tissues. Yet, heterogeneity in protocols limits cross‐study comparability and clinical translation. Here, we review α‐synuclein SAA methods and their performance across various biological matrices.
Manuela Amaral‐do‐Nascimento +3 more
wiley +1 more source
Frontiers in Marine Science, 2023 The occurrence and seasonality of the Critically Endangered great hammerhead shark, Sphyrna mokarran, is data deficient in the Central Pacific region.
Tatiana Boube +31 more
doaj +1 more source