Results 81 to 90 of about 287,947 (285)

The acidic domain of the endothelial membrane protein GPIHBP1 stabilizes lipoprotein lipase activity by preventing unfolding of its catalytic domain. [PDF]

, 2016
GPIHBP1 is a glycolipid-anchored membrane protein of capillary endothelial cells that binds lipoprotein lipase (LPL) within the interstitial space and shuttles it to the capillary lumen.
Beigneux, Anne P, Bensadouen, André, Fong, Loren G, Gårdsvoll, Henrik, Jørgensen, Thomas Jd, Kristensen, Kristian Kølby, Larsson, Mikael, Mysling, Simon, Ploug, Michael, Young, Stephen G   +9 more
core   +4 more sources

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Targeted-pig trial on safety and immunogenicity of serum-derived extracellular vesicles enriched fractions obtained from Porcine Respiratory and Reproductive virus infections [PDF]

, 2018
The Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) is the etiological agent of one of the most important swine diseases with a significant economic burden worldwide.
Borrás, Francesc E., del Portillo, Hernando A., Fraile Sauce, Lorenzo José, Montaner Tarbes, Sergio Roberto, Montoya, Maria, Novell, Elena, Tarancón, Vicens   +6 more
core   +4 more sources

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Validation of Protein A chromatography: orthogonal method with size exclusion chromatography validation for mAb titer analysis

Türk Biyokimya Dergisi
After reviewing the reference product specifications, found that they include the SEC HPLC result but do not specify Protein A chromatography as a test.
Sahinkaya Elif, Ozkan Aylin, Ozdemir Nilay, Aslan Merve Elif, Bicak Dilan, Cakir Nilufer, Oncel Hatice, Yetimoglu Ece Kok   +7 more
doaj   +1 more source

Development of comprehensive liquid chromatography (LC x LC) approaches for the analysis of complex copolymer structures [PDF]

, 2012
Knowledge of the exact composition and purity of advanced copolymers is of utmost importance given their influence on the final physical properties. The development of analytical techniques to examine these impurities is therefore crucial with respect of
Du Prez, Filip, Lynen, Frederic, Sandra, Patrick, Van Hoeylandt, Tim   +3 more
core   +1 more source

The dynamic action of SecA during the initiation of protein translocation [PDF]

, 2013
Biotechnology and Biological Sciences Research Council (BBSRC) [a doctoral training grant Ph.D. studentship to S.W. and project grant number BB/I008675/1] and the Wellcome Trust [project grant number 084452]
Alice Robson, Bauer, Blobel, Breyton, Brundage, Cannon, Deville, Ding, Erlandson, Gelis, Gold, Gold, Gouridis, Hizlan, Hunt, Ian Collinson, Kimura, Or, Osborne, Osborne, Park, Plath, Robson, Robson, Sarah Whitehouse, van den Berg, Vicki A. M. Gold, Whitehouse, Woodbury, Zimmer, Zimmer   +30 more
core   +2 more sources

Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum

FEBS Open Bio, EarlyView.
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik, Russell Pickford, Hannah C. Timmins, Olivier Piguet, Glenda M. Halliday, Matthew C. Kiernan, Woojin Scott Kim   +6 more
wiley   +1 more source

Recombinant protein immobilisation and display by alginate : a thesis presented in partial fulfilment of the requirements of the degree of Master of Science in Microbiology at Massey University, Palmerston North, New Zealand. [PDF]

, 2016
Biopolymers are a diverse group of organic materials with important applications in a number of industries. Their ability to adsorb and encapsulate compounds has been widely utilised in both biotechnologies and pharmaceuticals.
Jameson, Andrew
core  

Rapid online buffer exchange for screening of proteins, protein complexes and cell lysates by native mass spectrometry [PDF]

, 2020
It is important to assess the identity and purity of proteins and protein complexes during and after protein purification to ensure that samples are of sufficient quality for further biochemical and structural characterization, as well as for use in ...
Baker, David, Boyken, Scott E., Busch, Florian, Chen, Zibo, Jia, Mengxuan, Jones, Benjamin J., Sahasrabuddhe, Aniruddha, VanAernum, Zachary L., Wysocki, Vicki H.   +8 more
core