Results 21 to 30 of about 372,530 (379)

Comprehensive characterization and quantification of adeno associated vectors by size exclusion chromatography and multi angle light scattering

open access: yesScientific Reports, 2021
Adeno associated virus (AAV) capsids are a leading modality for in vivo gene delivery. Complete and precise characterization of capsid particles, including capsid and vector genome concentration, is necessary to safely and efficaciously dose patients ...
N. McIntosh   +8 more
semanticscholar   +1 more source

Establishment of a simplified dichotomic size‐exclusion chromatography for isolating extracellular vesicles toward clinical applications

open access: yesJournal of Extracellular Vesicles, 2021
Size‐exclusion chromatography (SEC) is a widely adopted method for the isolation of extracellular vesicles (EVs) from complex samples. SEC can efficiently remove high‐abundant proteins, while often requires multiple fractionation operation using ...
Jiahui Guo   +7 more
semanticscholar   +1 more source

Quantitative proteomic analysis of extracellular vesicle subgroups isolated by an optimized method combining polymer‐based precipitation and size exclusion chromatography

open access: yesJournal of Extracellular Vesicles, 2021
The molecular characterization of extracellular vesicles (EVs) has revealed a great heterogeneity in their composition at a cellular and tissue level.
J. A. Martínez-Greene   +10 more
semanticscholar   +1 more source

A deadenylase assay by size-exclusion chromatography. [PDF]

open access: yesPLoS ONE, 2012
The shortening of the 3'-end poly(A) tail, also called deadenylation, is crucial to the regulation of mRNA processing, transportation, translation and degradation.
Guang-Jun He, Yong-Bin Yan
doaj   +1 more source

Extracellular vesicles isolated by size-exclusion chromatography present suitability for RNomics analysis in plasma

open access: yesJournal of Translational Medicine, 2021
Background Extracellular vesicles (EVs), known as cell-derived membranous structures harboring a variety of biomolecules, have been widely used in liquid biopsy.
Yang Yang   +7 more
semanticscholar   +1 more source

Microfluidic Size Exclusion Chromatography (μSEC) for Extracellular Vesicles and Plasma Protein Separation.

open access: yesSmall, 2022
Extracellular vesicles (EVs) are recognized as next generation diagnostic biomarkers due to their disease-specific biomolecular cargoes and importance in cell-cell communications.
Sheng Yuan Leong   +8 more
semanticscholar   +1 more source

A Review of Exosomal Isolation Methods: Is Size Exclusion Chromatography the Best Option?

open access: yesInternational Journal of Molecular Sciences, 2020
Extracellular vesicles (EVs) are membranous vesicles secreted by both prokaryotic and eukaryotic cells and play a vital role in intercellular communication.
Karim Sidhom, Patience O. Obi, A. Saleem
semanticscholar   +1 more source

Isolation and Quantification of Polystyrene Nanoplastics in Tissues by Low Pressure Size Exclusion Chromatography

open access: yesJournal of Xenobiotics, 2022
Ecotoxicity investigations of plastic nanoparticles (NPs) should pay more attention to their ability to pass barriers, accumulate, and initiate toxicity in cells.
François Gagné
doaj   +1 more source

Expression, Purification and Characterization of Functional Teduglutide Using GST Fusion System in Prokaryotic Cells [PDF]

open access: yesAdvanced Pharmaceutical Bulletin, 2023
Purpose: Teduglutide is the first and only FDA-approved drug for long-term treatment of short bowel syndrome (SBS). The current study aimed to present an approach for production of teduglutide using recombinant DNA technology.
Ali Akbar Alizadeh   +4 more
doaj   +1 more source

Partial Purification of Peroxiredoxin-2 From Porcine Skeletal Muscle

open access: yesMeat and Muscle Biology, 2021
Fresh meat quality is adversely affected by protein oxidation. However, a fundamental understanding of the diverse factors that influence protein oxidation in postmortem muscle remains elusive.
Edward M;. Steadham   +3 more
doaj   +2 more sources

Home - About - Disclaimer - Privacy