Purification of bacteriocins using size-exclusion chromatography
Bangladesh Journal of Pharmacology, 2016 The bacteriocin purification involves following main steps. a). Extraction of cell-free-supernatant of bacteria. b). Ammonium sulfate precipitation. c). Dialysis. d). Diafiltration using PVP and e). Size-exclusion chromatography.
Vivek K. Bajpai +2 more
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Size exclusion chromatography for extraction of serum bile acids.
Journal of Lipid Research, 1990 A major problem in the measurement of serum bile acids is their quantitative extraction from the high molecular protein matrix. In our hands, the standard techniques of adsorption and reversed-phase chromatography have yielded incomplete recovery for ...
R Nuber, H Maucher, EF Stange
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Size exclusion chromatography as green support for forced degradation study of adalimumab
Acta Pharmaceutica, 2023 Size exclusion chromatography (SEC) has become a powerful tool for analysing size variants of biologic drugs in their native form. Modern SEC can be defined by the use of chromatographic columns packed with sub-3 µm particles, allowing an increase in ...
Kovačić Jelena +3 more
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Uncertainty of Size-Exclusion Chromatography Method in Quality Control of Bevacizumab Batches
Separations, 2021 In addition to the analytical challenges related to the size and complexity of biopharmaceutical drugs, the inherent variability that arises due to their manufacturing process requires monitoring throughout the production process to ensure the safety and
Alexis Oliva, Matías Llabrés
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HEMA/MMA/EDMA packing material evaluation for size exclusion chromatography (SEC)
Materials Research, 2005 HEMA/MMA/EDMA (2-hydroxyethyl methacrylate/methyl methacrylate/ethyleneglycol methacrylate) copolymer beads have been synthesized for use in aqueous SEC.
Cristina Duarte Vianna-Soares +2 more
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Determination of Hydrodynamic Radius of Proteins by Size Exclusion Chromatography
Bio-Protocol, 2017 Size exclusion chromatography (SEC) or gel filtration is a hydrodynamic technique that separates molecules in solution as a function of their size and shape. In the case of proteins, the hydrodynamic value that can be experimentally derived is the Stokes
Valentina La Verde +2 more
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The Use of Size Exclusion Chromatography to Monitor Protein Self-Assembly
Crystals, 2017 High resolution size exclusion chromatography (SEC) coupled with static light scattering (SLS) analyses were conducted to study the effect of the mobile phase ionic strength and protein concentration on the output of SEC experiments.
Alaa Adawy, Matthew R. Groves
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Single-step isolation of extracellular vesicles by size-exclusion chromatography
Journal of Extracellular Vesicles, 2014 Background: Isolation of extracellular vesicles from plasma is a challenge due to the presence of proteins and lipoproteins. Isolation of vesicles using differential centrifugation or density-gradient ultracentrifugation results in co-isolation of ...
Anita N. Böing +5 more
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High-grade extracellular vesicles preparation by combined size-exclusion and affinity chromatography
Scientific Reports, 2021 Extracellular vesicles (EVs) have recently gained growing interest for their diagnostic and therapeutic potential. Despite this, few protocols have been reported for the isolation of EVs with preserved biological function.
Cristina Bellotti +4 more
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Biospeciation of Cr(III) Nutritional Supplements in Biological Fluids
Makara Journal of Science, 2017 Metal ion speciation in various biological systems has been extensively studied to elucidate its biological role and the toxicity of the element of interest.
Anna Safitri, Aviva Levina, Peter A. Lay
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