Results 21 to 30 of about 158,973 (248)
Biological Procedures Online, 2020 Background Circulating small extracellular vesicles (sEVs) and its associated proteins are of great interest in the early detection of many diseases. However, there is no gold standard for plasma sEVs isolation, especially for proteomic profiling which ...
Rui Wei +6 more
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Separation of whey proteins for chromatography liquid
Scientia Agropecuaria, 2010 This paper describes and compares three chromatographic methods for the analysis and quantification of most abundant proteins in cheese whey, -lactalbumin and -lactoglobulin. The methods were: Reverse-phase high performance liquid chromatography, anion
Abraham D. Giraldo Zuñiga +3 more
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Framework for rapid comparison of extracellular vesicle isolation methods
eLife, 2021 Extracellular vesicles (EVs) are released by all cells into biofluids and hold great promise as reservoirs of disease biomarkers. One of the main challenges in studying EVs is a lack of methods to quantify EVs that are sensitive enough and can ...
Dmitry Ter-Ovanesyan +6 more
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Molecular Dispersion of Starch as a Crucial Parameter during Size-Exclusion Chromatography
Foods, 2020 Starch, α-polyglucan consisting of a large number of anhydroglucose units joined by α-1,4- and α-1,6-glycosidic bonds, seems to be characterized by a simple structure when compared to other natural polymers.
Artur Szwengiel, Piotr Kubiak
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Biomolecules, 2023 Follicular fluid (FF) is the microenvironment where a growing oocyte develops. Intrafollicular communication ensures oocyte competence and is carried out through paracrine signaling, the exchange of molecules via gap junctions, and the trafficking of ...
Maria Soares +7 more
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Amylose Inter-Chain Entanglement and Inter-Chain Overlap Impact Rice Quality
Foods, 2022 Retrogradation of cooked rice happens in two ways: one is by the formation of ordered structures, and the other is through intra- and inter-chain entanglement and inter-chain overlap, which in turn are affected by the amylose chain-length distribution ...
Changfeng Li +6 more
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Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
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Polish Polar Research, 2020 Humic substances are polydisperse mixtures of structurally complex matters with different molecular weights. The complexity of molecular composition of humic substances is reflected through their physical and chemical properties and results in diverse ...
Lodygin, Evgeny, Vasilevich, Roman
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BMC Urology, 2021 Background Because of their specific and biologically relevant cargo, urine extracellular vesicles (EVs) constitute a valuable source of potential non-invasive biomarkers that could support the clinical decision-making to improve the management of ...
María García-Flores +5 more
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Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source