IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo +6 more
wiley +1 more source
Ocular and skin temperature in ophthalmic postherpetic neuralgia [PDF]
Non-contact infra-red thermography was employed to measure the temperature of the eye and facial skin of a group of 12 patients with postherpetic neuralgia 5 months or more after the onset of ophthalmic shingles.
Morgan, Philip B. +7 more
core
Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow +12 more
wiley +1 more source
A global climatology of the diurnal variations in sea-surface temperature and implications for MSU temperature trends [PDF]
A global climatology of diurnal variations in sea-surface temperature based on in situ drifting-buoy data has been created. The diurnal warming signal derived from these data correlates well with estimates from a version of the Stuart-Menteth (2004 ...
Kennedy, J. J. +2 more
core +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Measuring temperature of the ice surface during its formation by using infrared instrumentation [PDF]
A non-destructive remote sensing technique was used to measure the surface temperature of a thin macroscopic water film flowing on a growing asymmetric ice accretion during its formation inside an icing research wind tunnel.
Kollar, László E. +2 more
core +1 more source
Developmental and Epileptic Encephalopathy due to Biallelic Pathogenic Variants in PIGM
ABSTRACT Objective PIGM encodes a critical enzyme in the glycosylphosphatidylinositol (GPI)‐anchor biosynthesis pathway. While promoter‐region mutations in PIGM have been associated with a relatively mild phenotype characterized by portal vein thrombosis and absence seizures, recent evidence suggests that coding‐region mutations result in a more severe
Júlia Sala‐Coromina +11 more
wiley +1 more source
Skin-to-skin contact and suckling in early postpartum : effects on temperature, breastfeeding and mother-infant interaction [PDF]
The overall aim of this thesis was to explore the role of closeness versus separation on infant and maternal temperature adaptation, breastfeeding outcome and mother-infant interaction. In addition, we aimed to study a potential influence of swaddling on
Ksenia Bystrova (19493989)
core
Effects of Whole Body Cryotherapy and Cold Water Immersion on Knee Skin Temperature [PDF]
This study sought to a) compare and contrast the effect of 2 commonly used cryotherapy treatments, 4 min of −110°C whole body cryotherapy and 8°C cold water immersion, on knee skin temperature and b) establish whether either protocol was capable of ...
Donnelly, A. +10 more
core +1 more source

