A hyperactive
BMC Research Notes, 2010 Background Transposons are useful molecular tools for transgenesis. The 'sleeping beauty' transposon is a synthetic member of the Tc1/mariner transposon family. Davidson et al. (2003) previously described a vector for zebrafish transgenesis consisting of
Lardelli Michael, Newman Morgan
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The Rijksmuseum Bulletin, 2019 The interest in fairy tales grew strongly over the course of the nineteenth century, particularly in Germany, the birthplace of Frans Stracké (1820-1898).
Margreet Boomkamp
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Understanding cancer drug resistance with Sleeping Beauty functional genomic screens: Application to MAPK inhibition in cutaneous melanoma [PDF]
iScience, 2023 Summary: Combined BRAF and MEK inhibition is an effective treatment for BRAF-mutant cutaneous melanoma. However, most patients progress on this treatment due to drug resistance.
Eliot Y. Zhu +4 more
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Using the Sleeping Beauty Transposon System for Doxycycline-inducible Gene Expression in RAW264.7 Macrophage Cells to Study Phagocytosis [PDF]
Bio-ProtocolMacrophages are known for engulfing and digesting pathogens and dead cells through a specialized form of endocytosis called phagocytosis. Unfortunately, many macrophage cell lines are refractory to most reagents used for transient transfections ...
Parsa Kamali, Gregory Fairn
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Ring stage dormancy of Plasmodium falciparum tolerant to artemisinin and its analogues – A genetically regulated “Sleeping Beauty” [PDF]
International Journal for Parasitology: Drugs and Drug Resistance, 2023 The appearance in 2008 in western Cambodia of Plasmodium falciparum tolerant to artemisinin, defined by longer parasite clearance time following drug administration and in vitro by a slightly higher survival rate of the ring stage after a 3-h treatment ...
Saranya Auparakkitanon, Prapon Wilairat
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Targeted delivery of a PD-1-blocking scFv by CD133-specific CAR-T cells using nonviral Sleeping Beauty transposition shows enhanced antitumour efficacy for advanced hepatocellular carcinoma [PDF]
BMC Medicine, 2023 Background CD133 is considered a marker for cancer stem cells (CSCs) in several types of tumours, including hepatocellular carcinoma (HCC). Chimeric antigen receptor-specific T (CAR-T) cells targeting CD133-positive CSCs have emerged as a tool for the ...
Chaopin Yang +22 more
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Sleeping beauty or wide awake?
Scandinavian Journal for Leadership & Theology, 2023 This article studies mission within the context of traditional Nordic national churches. In the Evangelical Lutheran Church of Norway, a state church until 2012, eighty percent of all congregations have formal agreements with a mission organization to ...
Stephen Sirris, Hans Austnaberg
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Sleeping Beauty’s Credences [PDF]
Philosophy of Science, 2016 The Sleeping Beauty problem has spawned a debate between “thirders” and “halfers” who draw conflicting conclusions about Sleeping Beauty's credence that a coin lands heads. Our analysis is based on a probability model for what Sleeping Beauty knows at each time during the experiment.
Cisewski, Jessi +4 more
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‘Both Hero and Villain’ – Rewriting the Tale, Revising the Villain, and Retelling Gender in Disney’s 'Maleficent' (2014) [PDF]
Limina: A Journal of Historical and Cultural Studies, 2021 Fairy tale films have always been popular but since the beginning of the twenty-first century many well-known fairy tales have been once again retold and refashioned.
Svea Hundertmark
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The folding of the specific DNA recognition subdomain of the sleeping beauty transposase is temperature-dependent and is required for its binding to the transposon DNA. [PDF]
PLoS ONE, 2014 The reaction of DNA transposition begins when the transposase enzyme binds to the transposon DNA. Sleeping Beauty is a member of the mariner family of DNA transposons.
Gage O Leighton +5 more
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