Enhancing CHO cell recombinant protein production using a perfusion‐directed host evolution approach
Abstract Clonally derived cell lines generated from Chinese hamster ovary (CHO) cells encounter numerous stressors when cultured in high‐intensity perfusion bioreactors leading to poor process performance. To circumvent this, the ability of CHO cells to adapt to different culture environments was exploited. Here host cells were selected in the presence
Peter Amaya +7 more
wiley +1 more source
Various methods to detect small GTPase activation: from radioisotope-based methods to the Small GTPase ActIvitY ANalysing (SAIYAN) system. [PDF]
Maeda M, Saito K.
europepmc +1 more source
PP2A-Mediated GSK3β Dephosphorylation Is Required for Protocadherin-7-Dependent Regulation of Small GTPase RhoA in Osteoclasts. [PDF]
Kim H, Takegahara N, Choi Y.
europepmc +1 more source
Interaction Between Actin and Microtubules During Plant Development
ABSTRACT The dynamic interaction between actin filaments (AFs) and microtubules (MTs) plays a crucial role in regulating key developmental and physiological processes in plant cells, particularly in the formation of specialized cell types with distinct shapes and functions, such as pollen tubes, trichomes, and leaf epidermal cells.
Zining Wu +3 more
wiley +1 more source
Editorial: Energy Requirements in Membrane Trafficking
Carlos M. Guardia +3 more
doaj +1 more source
Split-Small GTPase Reassembly as a Method to Control Cellular Signaling with User-Defined Inputs. [PDF]
He Y +4 more
europepmc +1 more source
The alarmone ppGpp selectively inhibits the isoform A of the human small GTPase Sar1. [PDF]
Huang Q, Szebenyi DME.
europepmc +1 more source
ABSTRACT Of the three types of cytoskeleton known in animals—actin, microtubules, and intermediate filaments—only actin and microtubules exist in plants. Both play important roles in cellular shaping, organelle movement, organization of the endomembrane system, and cell signaling.
Norman R. Groves +3 more
wiley +1 more source
The mitotic exit mediated by small GTPase Tem1 is essential for the pathogenicity of Fusarium graminearum. [PDF]
Miao P +8 more
europepmc +1 more source
Making Two out of One: Kinesin Motors Driving Plant Cell Division
ABSTRACT The Kinesin superfamily of microtubule dependent motors is present in all eukaryotes. Not all of the subfamilies are represented in all kingdoms, and the ones that are do not always show conserved functions. Tight control of the cytoskeleton is essential for proper progression and completion of mitosis and cytokinesis, and key functions are ...
Choy Kriechbaum, Sabine Müller
wiley +1 more source

