Results 121 to 130 of about 875 (144)
Development of specific diagnostic test for small ruminant lentivirus genotype E
Veterinary Microbiology, 2009 Small ruminant lentivirus (SRLV) belonging to the highly divergent genotype E has recently been identified in the Italian goat breed Roccaverano. In this report we have developed a specific serological test based on recombinant matrix/capsid antigen fusion protein.
Ramses Reina +2 more
exaly +4 more sources
Compartmentalization of small ruminant lentivirus between blood and colostrum in infected goats
Virology, 2007 The compartmentalization of small ruminant lentivirus (SRLV) subtype A (Maedi-Visna virus) and B (caprine arthritis-encephalitis virus) variants was analyzed in colostrum and peripheral blood mononuclear cells of four naturally infected goats. Sequence analysis of DNA and RNA encompassing the V4-V5 env regions showed a differential distribution of SRLV
Giuliano Pisoni +2 more
exaly +5 more sources
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Detection and isolation of small ruminant lentivirus in the amniotic fluid of goats
Comparative Immunology, Microbiology and Infectious Diseases, 2021The objective of this study was to verify the presence of small ruminant lentivirus in the amniotic fluid of goats using molecular tests and viral isolation by cocultivation in the amniotic fluid of naturally infected goats. The study analyzed eight goats: seven were small ruminant lentivirus-positive and one was negative.
Juscilânia Furtado Araújo +10 more
openaire +2 more sources
Serological study of small ruminant lentivirus in goats in Italy
Small Ruminant Research, 2007Abstract Objective To determine seroprevalence of small ruminant lentivirus (SRLV) infections among goats in South Tyrol, Italy. Design Prospective serological study. Material and methods-animals 15,980 goats older than 6 months from 1973 goat herds in South Tyrol were tested by an ELISA for the presence of SRLV antibodies. 3148 goats were 6–
H Gufler
exaly +2 more sources
Characterization of an immunodominant epitope of small ruminant lentivirus (SRLV) nucleoprotein
Veterinary Immunology and Immunopathology, 2008To extend and complete the epitope mapping of gag-encoded structural proteins, the immunodiagnostic potential of nucleoprotein (NP) of two different small ruminant lentivirus (SRLV) genotypes were antigenically characterized. Respective recombinant counterparts were generated and used in an enzyme-linked immunosorbent assay (ELISA) format to test a ...
D Nucera, Sérgio Rosati
exaly +3 more sources
Antibody-based diagnosis of small ruminant lentivirus infection in seminal fluid
Theriogenology, 2009 Antibody-based diagnosis of small ruminant lentiviruses (SRLVs) has been efficiently achieved using serum and milk, but not semen, for which polymerase chain reaction (PCR) has been proposed as a confirmatory technique. This work, involving 296 ovine (Ovis aries) and caprine (Capra hircus) semen donors, investigates whether seminal fluid (SF) can be ...
Hugo Ramirez Alvarez +2 more
exaly +4 more sources
Decline of maternal antibodies to small ruminant lentivirus in goat kids
Animal Science Journal, 2018AbstractWe carried out this study to determine for how long small ruminant lentivirus (SRLV)‐specific antibodies can be detected by three commercialELISAkits in goat kids after suckling infected does in field conditions. Forty‐one kids born toSRLV‐seropositive asymptomatic does were blood sampled prior to colostrum consumption, and then weekly for 6 ...
Michał Czopowicz +8 more
openaire +2 more sources
Influence of small ruminant lentivirus infection on cheese yield in goats
Journal of Dairy Research, 2014Three-year cohort study was carried out to investigate the influence of small ruminant lentivirus (SRLV) infection on cheese yield in goats. For this purpose records of milk yield, milk composition and cheese yield were collected in a dairy goat herd. Cheese yield was recorded as the amount of fresh cheese obtained from 1 kg milk.
Dorota, Nowicka +5 more
openaire +2 more sources
Small Ruminant Research, 2013
Abstract A compulsory control program to control-eradicate Brucella infections was initiated 1985 in the Basque Country in Northern Spain. The program included both Brucella melitensis infection (ovine/caprine brucellosis), which were later included in a national plan, and Brucella ovis infection (ovine epididymitis), which was promoted by the ...
R.A. Juste +8 more
openaire +1 more source
Abstract A compulsory control program to control-eradicate Brucella infections was initiated 1985 in the Basque Country in Northern Spain. The program included both Brucella melitensis infection (ovine/caprine brucellosis), which were later included in a national plan, and Brucella ovis infection (ovine epididymitis), which was promoted by the ...
R.A. Juste +8 more
openaire +1 more source
Improvements in the detection of small ruminant lentivirus infection in the blood of sheep by PCR
Journal of Virological Methods, 2009The polymerase chain reaction on blood samples has been considered a complement to serological methods for the detection of small ruminant lentiviruses (SRLV) infections in sheep and goats. This is a report on the results of a study to evaluate the use of the same blood sample for the detection of infected animals by ELISA and PCR. A comparison between
I, Leginagoikoa +3 more
openaire +2 more sources