Results 131 to 140 of about 6,254 (216)

Single-molecule imaging and molecular dynamics simulations reveal early activation of the MET receptor in cells

Nature Communications
Embedding of cell-surface receptors into a membrane defines their dynamics but also complicates experimental characterization of their signaling complexes.
Yunqing Li, Serena M. Arghittu, Marina S. Dietz, Gabriel J. Hella, Daniel Haße, Davide M. Ferraris, Petra Freund, Hans-Dieter Barth, Luisa Iamele, Hugo de Jonge, Hartmut H. Niemann, Roberto Covino, Mike Heilemann   +12 more
doaj   +1 more source

Automated Analysis of Dynamic Single Molecule Experiments [PDF]

, 2018
Single molecule total internal reflection fluorescence studies using co-localization and fluorescence resonance energy transfer have become valuable tools to study biological phenomenon.
London, James
core  

The potential of fluorogenicity for single molecule FRET and DyeCycling

QRB Discovery
Single Molecule Förster Resonance Energy Transfer (smFRET) is a popular technique to directly observe biomolecular dynamics in real time, offering unique mechanistic insight into proteins, ribozymes, and so forth.
Srijayee Ghosh, Sonja Schmid
doaj   +1 more source

CLC Conformational Landscape as Studied by SMFRET [PDF]

Biophysical Journal, 2019
Ayush Krishnamoorti, Ricky C. Cheng, Vladmir Berka, Merritt Maduke   +3 more
openaire   +1 more source

Paving the way for structural modelling by smFRET measurements

, 2019
The thesis at hand contributes to the field of structure determination of bio-macromolecules. It focuses on the Förster resonance energy transfer (FRET), which allows to infer distances at the molecular level (≈ 2-10 nm) making FRET a promising tool for structure analysis.
openaire   +2 more sources

Ligand response of guanidine-IV riboswitch at single-molecule level

eLife
Riboswitches represent a class of non-coding RNA that possess the unique ability to specifically bind ligands and, in response, regulate gene expression.
Lingzhi Gao, Dian Chen, Yu Liu
doaj   +1 more source

Time heterogeneity of the Förster radius from dipole orientational dynamics impacts single-molecule Förster resonance energy transfer experiments

Physical Review Research
Förster resonance energy transfer (FRET) is a quantum mechanical process governing the nonradiative energy transfer between coupled electric dipoles. Its strong distance dependence makes it a widely used as a “molecular ruler” in biology, chemistry, and ...
David Frost, Keisha Cook, Hugo Sanabria
doaj   +1 more source

Advancing Understanding of DNA-BfiI Restriction Endonuclease Cis and Trans Interactions through smFRET Technology

bioRxiv, 2023
Š. Ivanovaitė, Justė Paksaitė, Aurimas Kopūstas, Giedrė Karzaitė, D. Rutkauskas, A. Šilanskas, G. Sasnauskas, M. Zaremba, Stephen K. Jones, Marijonas Tutkus   +9 more
semanticscholar   +1 more source

Integration of smFRET and molecular dynamics to characterize conformational dynamics of the spike protein of wild-type SARS-CoV-2 and its variants

Biophysical Journal, 2023
Joseph Williamson, Maolin Lu, Mahmoud Moradi   +2 more
semanticscholar   +1 more source

Simulating Freely Diffusing Single-Molecule FRET Data with Consideration of Protein Conformational Dynamics. [PDF]

J Phys Chem B
Losey J, Jauch M, Cortes-Cubero A, Wu H, Polasa A, Sauve S, Rivera R, Matteson DS, Moradi M.   +8 more
europepmc   +1 more source