Physical Review ResearchFörster resonance energy transfer (FRET) is a quantum mechanical process governing the nonradiative energy transfer between coupled electric dipoles. Its strong distance dependence makes it a widely used as a “molecular ruler” in biology, chemistry, and ...
David Frost, Keisha Cook, Hugo Sanabria
doaj +1 more source
Observation of SAM-VI Riboswitch Dynamics Using Single-Molecule FRET
BiomoleculesRiboswitches regulate gene expression through intricate dynamic conformational transitions, with divalent cation Mg2+ and their ligands playing pivotal roles in this process.
Yanyan Xue +4 more
doaj +1 more source
smFRET and biochemical data for the study of PPR binding to single-stranded RNA
<p>Dataset contains (predominantly) pre-processed data for single-molecule FRET (smFRET) and other biochemical data (e.g., SDS-PAGE gels, surface plasmon resonance experiments, FPLC chromatograms).
Marzano, Nicholas
core +1 more source
Realtime Kinetics of in-vitro RNA Polymerase Transcription
, 2016 smFRET realtime kinetics experiments of RNAP transitioning from initiation to elongation.Measurements were performed both on a 8-spot smFRET system and a single-spot usALEX-smFRET system.
Xavier Michalet (2570443) +2 more
core +1 more source
An smFRET assay to probe the impact of antibiotics on intersubunit rotation in eukaryotic ribosomes
Protein translation involves large-scale structural rearrangements within the ribosome. During translation elongation, the two subunits of the ribosome rotate with respect to each other to allow for tRNA-mRNA translocation. While conformational changes of bacterial ribosomes have been extensively explored using cryo-EM and single-molecule microscopy ...
Grove, Amy K. +4 more
openaire +2 more sources
A Multicolor Single-Molecule FRET Approach to Study Protein Dynamics and Interactions Simultaneously
, 2016 Single-molecule Förster resonance energy transfer (smFRET) is a versatile tool for studying biomolecules in a quantitative manner. Multiple conformations within and interactions between biomolecules can be detected and their kinetics can be determined ...
P. Wortmann +11 more
core +1 more source
ATP-driven conformational dynamics reveal hidden intermediates in a heterodimeric ABC transporter. [PDF]
ElifePečak M, Nocker C, Tampé R.
europepmc +2 more sources
Recovering true FRET efficiencies from smFRET investigations requires triplet state mitigation
<p>This includes</p> <p>1- the data set collected at 100mW = 0.04 kW/cm^2 for the Cy3-Cy5 dye pair that is reported in the paper in Figure 2c and SI Figure 14ab</p> <p>2- Matlab script to do the simplified zeta correction ...
Blanchard, Scott C. +9 more
core +1 more source
Structural Dynamics of SNARE Complex Assembly in the Ribbon Synapses Observed by smFRET
Single-molecule fluorescence resonance energy transfer (smFRET) is a powerful technique for studying the structural dynamics of protein molecules or detecting interactions between protein molecules in real time. Due to the high sensitivity in spatial and temporal resolution, smFRET can decipher sub-populations within heterogeneous native state ...
Andrzejewski, Alexa L. +4 more
openaire +2 more sources
Ligand-tuned extracellular domain dynamics are correlated with signaling states in an adhesion GPCR. [PDF]
Sci AdvCechova K +9 more
europepmc +1 more source