Results 111 to 120 of about 4,596 (191)

Investigation of structural dynamics of H/ACA RNPs by smFRET spectroscopy & design and applications of versatile small molecule dependent RNA of interest release platform

, 2019
This dissertation contains two chapters. Each chapter covers a unique topic within RNA science and is divided in two sub sections, part A and B. Each chapter contains an introduction.
Schmidt, Andreas
core  

An smFRET assay to probe the impact of antibiotics on intersubunit rotation in eukaryotic ribosomes

Protein translation involves large-scale structural rearrangements within the ribosome. During translation elongation, the two subunits of the ribosome rotate with respect to each other to allow for tRNA-mRNA translocation. While conformational changes of bacterial ribosomes have been extensively explored using cryo-EM and single-molecule microscopy ...
Grove, Amy K., Das, Ananya, Wakabayashi, Hironao, Ivanov, Aleksandr V., Ermolenko, Dmitri N.   +4 more
openaire   +2 more sources

Alternation histograms for μs-ALEX and ns-ALEX measurements.

, 2016
Histograms used for the selection/determination of the alternation periods for two typical smFRET-ALEX experiments. Distributions of photons detected by donor channel are in green, and by acceptor channel in red.
SangYoon Chung (2993058), Antonino Ingargiola (2993055), Eitan Lerner (2808022), Xavier Michalet (1630444), Shimon Weiss (1290570)   +4 more
core   +1 more source

Operational regime of different FRET methods.

, 2013
Conventional smFRET and fluorometric assays are difficult to perform in the intermediate concentration regime (100 pM to 1 nM, shaded area). Quasi-bulk smFRET and microplate-scanning FRET (μpsFRET), on the contrary, allow us to accurately determine FRET ...
Vera Böhm (404273), Aaron R. Hieb (352787), Katalin Tóth (173523), Jörg Langowski (173529), Alexander Gansen (404272)   +4 more
core   +1 more source

Prism-Based smFRET TIRF Analysis Shows Dynamic and Transient Interactions in Free Fulllength NFκB, Potentially Due to the Presence of the Disordered Transcription Activation Domain [PDF]

Single molecule Förster resonance energy transfer (smFRET) is a powerful biophysical structural method for investigating the structural dynamics and interactions of biomolecules at the single-molecule level. By utilizing fluorophores strategically placed
Yassir, Noura
core   +1 more source

Time heterogeneity of the Förster radius from dipole orientational dynamics impacts single-molecule Förster resonance energy transfer experiments

Physical Review Research
Förster resonance energy transfer (FRET) is a quantum mechanical process governing the nonradiative energy transfer between coupled electric dipoles. Its strong distance dependence makes it a widely used as a “molecular ruler” in biology, chemistry, and ...
David Frost, Keisha Cook, Hugo Sanabria
doaj   +1 more source

ATP-driven conformational dynamics reveal hidden intermediates in a heterodimeric ABC transporter. [PDF]

Elife
Pečak M, Nocker C, Tampé R.
europepmc   +2 more sources

The structural dynamics and molecular coupling in the slow inactivation of a prokaryotic voltage-gated sodium channel. [PDF]

Nat Commun
Irie K, Han S, Applewhite S, Maeda YK, Vance J, Wang S.   +5 more
europepmc   +1 more source

Distinct allosteric remodeling of HIV-1 Env dynamics on virions by gp41-directed antibodies reveals two modes of neutralization. [PDF]

Elife
Xu W, Gonepudi NK, Liu J, He Y, Katte R, Wang R, Awuah HB, Han Y, Zhang B, Yu J, Hu B, Ho DD, Acharya P, Kwong PD, Lu M.   +14 more
europepmc   +1 more source

Ligand-tuned extracellular domain dynamics are correlated with signaling states in an adhesion GPCR. [PDF]

Sci Adv
Cechova K, Bandekar SJ, Kordon SP, Foroutan M, Leon K, Siffer G, Schamber MR, Luiso M, Araç D, Vafabakhsh R.   +9 more
europepmc   +1 more source