Viruses, 2018 Viruses are simple agents exhibiting complex reproductive mechanisms. Decades of research have provided crucial basic insights, antiviral medication and moderately successful gene therapy trials. The most infectious viral particle is, however, not always
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Accurate Determination of Human CPR Conformational Equilibrium by smFRET Using Dual Orthogonal Noncanonical Amino Acid Labeling [PDF]
, 2019 Conjugation of fluorescent dyes to proteinsa prerequisite for the study of conformational dynamics by single-molecule (sm) FRETcan lead to substantial changes in a dye's photophysical properties, ultimately biasing the determination of inter-dye ...
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CLC Conformational Landscape as Studied by SMFRET [PDF]
Biophysical Journal, 2019 Ayush Krishnamoorti +3 more
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Probing Allostery in Oligomeric M2 Receptors using smFRET [PDF]
Biophysical Journal, 2021 Dennis D. Fernandes +2 more
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Raw data to make the figures of the article: The conserved lysine residue in transmembrane helix 5 is key for the cytoplasmic gating of the L-amino acid transporters Authors: Joana Fort*, Adrià Nicolàs-Aragó, Luca Maggi, Maria Martinez Molledo, Despoina
Fort Baixeras, Joana
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smFRET results on nucleosome stability are consistent to μpsFRET data.
, 2013 (A) Average proximity ratio calculated from all photons from double-labeled nucleosomes as a function of salt concentration. Photons from the donor and transfer channel were summed for all detected molecules, except donor-only and acceptor-only species. (
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The potential of fluorogenicity for single molecule FRET and DyeCycling
QRB DiscoverySingle Molecule Förster Resonance Energy Transfer (smFRET) is a popular technique to directly observe biomolecular dynamics in real time, offering unique mechanistic insight into proteins, ribozymes, and so forth.
Srijayee Ghosh, Sonja Schmid
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An Expanded Conformation of an Antibody Fab Region by X-Ray Scattering, Molecular Dynamics and smFRET Identifies an Aggregation Mechanism [PDF]
, 2019 Protein aggregation is the underlying cause of many diseases, and also limits the usefulness of many natural and engineered proteins in biotechnology. Better mechanistic understanding and characterization of aggregation-prone states, is needed to guide ...
Ahmad, SS +6 more
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Ligand response of guanidine-IV riboswitch at single-molecule level
eLifeRiboswitches represent a class of non-coding RNA that possess the unique ability to specifically bind ligands and, in response, regulate gene expression.
Lingzhi Gao, Dian Chen, Yu Liu
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Prism-Based smFRET TIRF Analysis Shows Dynamic and Transient Interactions in Free Fulllength NFκB, Potentially Due to the Presence of the Disordered Transcription Activation Domain [PDF]
Single molecule Förster resonance energy transfer (smFRET) is a powerful biophysical structural method for investigating the structural dynamics and interactions of biomolecules at the single-molecule level. By utilizing fluorophores strategically placed
Yassir, Noura
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