Results 81 to 90 of about 4,596 (191)

Resolving the dynamics of atypical DNA structures using smFRET

Current Opinion in Structural Biology
B-form DNA is a structural icon in biology. Noncanonical DNA conformations are increasingly recognized as essential drivers of genomic phenomena. These atypical structures, including hairpins, G-quadruplexes, and multistranded junctions, are often transient and highly dynamic, making them difficult to characterize using traditional methods.
Dorothy A, Erie, Sharonda J, LeBlanc, Keith R, Weninger   +2 more
openaire   +2 more sources

smFRET and DEER Distance Measurements as Applied to Disordered and Structured Proteins [PDF]

Biophysical Journal, 2016
FRET and DEER are two spectroscopic methods that are widely applied for biophysical studies of protein structure. Both methods are based on measuring dipolar interactions - electrical dipoles in case of FRET and magnetic dipoles in case of DEER - between specifically labeled protein sites.
Weninger, Keith, Qiu, Ruoyi, Ou, Erkang, Milikisiyants, Sergey, Sanabria, Hugo, Smirnova, Tatyana I.   +5 more
openaire   +1 more source

AI‐Physics‐Experiment Trinity for Integrated Protein Dynamics Modeling

Advanced Science, Volume 13, Issue 41, 22 July 2026.
This review unites experiments, physics‐based simulations, and AI as a synergistic triad for protein dynamics modeling. It highlights integrative strategies, resolves sampling and forcefield bottlenecks, and outlines challenges and future directions for accurate, interpretable conformational ensemble prediction.
Chen Shi, Minying Low, Peng Xiu, Kresten Lindorff‐Larsen, Yong Wang   +4 more
wiley   +1 more source

Toward dynamic structural biology:Two decades of single-molecule Forster resonance energy transfer [PDF]

, 2018
Classical structural biology can only provide static snapshots of biomacromolecules. Single-molecule Forster resonance energy transfer (smFRET) paved the way for studying dynamics in macromolecular structures under biologically relevant conditions. Since
Michalet, Xavier, SangYoon Chung, Eitan Lerner, Xavier Michalet, Weiss, Shimon, Antonino Ingargiola, Chung, SangYoon, Alhadid, Yazan, Thorben Cordes, Lerner, Eitan, Ingargiola, Antonino, Yazan Alhadid, Shimon Weiss, Cordes, Thorben, Ingargiol, Antonino   +14 more
core   +1 more source

A Systematic Approach for the Purification of Fluorophore‐Labeled Proteins via Anion Exchange Chromatography

ChemBioChem, Volume 27, Issue 11, 15 June 2026.
We present a rational purification strategy for stochastic donor–acceptor labeling of proteins for Förster resonance energy transfer (FRET) studies. By characterizing interactions of hydrolyzed fluorophore–maleimides with anion exchange matrices, we establish predictive rules for purification design.
Nicolas D. Wendler, Thorben Cordes
wiley   +1 more source

HIV-1-envelope trimer transitions from prefusion-closed to CD4-bound-open conformations through an occluded-intermediate state

Computational and Structural Biotechnology Journal
HIV-1 infection is initiated by the interaction between the gp120 subunit in the envelope (Env) trimer and the cellular receptor CD4 on host cells. This interaction induces substantial structural rearrangement of the Env trimer.
Myungjin Lee, Maolin Lu, Baoshan Zhang, Tongqing Zhou, Revansiddha Katte, Yang Han, Reda Rawi, Peter D. Kwong   +7 more
doaj   +1 more source

The Polymers of Life: Exploring Cellular Function Through Polymer Concepts

Advanced Science, Volume 13, Issue 33, 15 June 2026.
Biomolecular phase separation reveals that a hidden layer of cellular organization is governed by the principles of polymer science. This review bridges polymer physics and cell biology, offering a primer on fundamental concepts, proposing a framework for interrogating cellular function, and synthesizing biophysical methods for decoding macromolecular ...
Mark Chen, Ashutosh Chilkoti
wiley   +1 more source

Realtime Kinetics of in-vitro RNA Polymerase Transcription

, 2016
smFRET realtime kinetics experiments of RNAP transitioning from initiation to elongation.Measurements were performed both on a 8-spot smFRET system and a single-spot usALEX-smFRET system.
Xavier Michalet (2570443), Antonino Ingargiola (556170), Eitan Lerner (2808022)   +2 more
core   +1 more source

RNAs Associated With Bacterial Outer Membrane Vesicles: Structural Insights Into Surface Composition

Journal of Extracellular Vesicles, Volume 15, Issue 6, June 2026.
This work uncovers a new layer of bacterial communication. We show that RNAs, aided by the Hfq RNA‐chaperone, are not only packed inside Gram negative Outer Membrane Vesicles (OMVs) but can also be embedded in their membranes. This suggests that bacteria may use surface‐exposed RNAs to directly interact with host cells. Overall, our results broaden the
Kevin Mosca, Florian Turbant, Wafa Achouak, Frank Wien, Véronique Arluison   +4 more
wiley   +1 more source

Lipid composition controls the huntingtin exon 1 membrane‐association and differentially modulates its flanking regions' dynamics

Protein Science, Volume 35, Issue 6, June 2026.
Abstract The pathological expansion of the polyglutamine (polyQ) repeat within the first exon of huntingtin (Httex1) protein is a defining hallmark of Huntington's disease (HD). Multiple evidence supports that the membrane recruitment of Httex1 is critical for its self‐assembly and related toxicity in HD.
Tânia Sousa, Gonçalo Damas, Ana Coutinho, Nuno Bernardes, Ana Azevedo, Manuel Prieto, Ana M. Melo   +6 more
wiley   +1 more source