Results 81 to 90 of about 4,500 (194)

RNAs Associated With Bacterial Outer Membrane Vesicles: Structural Insights Into Surface Composition

Journal of Extracellular Vesicles, Volume 15, Issue 6, June 2026.
This work uncovers a new layer of bacterial communication. We show that RNAs, aided by the Hfq RNA‐chaperone, are not only packed inside Gram negative Outer Membrane Vesicles (OMVs) but can also be embedded in their membranes. This suggests that bacteria may use surface‐exposed RNAs to directly interact with host cells. Overall, our results broaden the
Kevin Mosca, Florian Turbant, Wafa Achouak, Frank Wien, Véronique Arluison   +4 more
wiley   +1 more source

smFRET and DEER Distance Measurements as Applied to Disordered and Structured Proteins [PDF]

Biophysical Journal, 2016
FRET and DEER are two spectroscopic methods that are widely applied for biophysical studies of protein structure. Both methods are based on measuring dipolar interactions - electrical dipoles in case of FRET and magnetic dipoles in case of DEER - between specifically labeled protein sites.
Weninger, Keith, Qiu, Ruoyi, Ou, Erkang, Milikisiyants, Sergey, Sanabria, Hugo, Smirnova, Tatyana I.   +5 more
openaire   +1 more source

An experiment-informed hierarchical Dirichlet process-hidden Markov model to analyze surface-immobilized smFRET data [PDF]

Experiments using single-molecule Forster resonance energy transfer (smFRET) techniques have revolutionized the study of conformational states in biological molecules such as intrinsically disordered proteins. Analysis of smFRET data often relies on Hid-
Hidalgo Vargas, Jared N.
core   +1 more source

Comparison of c_1/2 – values from μpsFRET and smFRET experiments.

, 2013
Comparison of c1/2 – values from μpsFRET and smFRET experiments.
Vera Böhm (404273), Aaron R. Hieb (352787), Katalin Tóth (173523), Jörg Langowski (173529), Alexander Gansen (404272)   +4 more
core   +1 more source

Lipid composition controls the huntingtin exon 1 membrane‐association and differentially modulates its flanking regions' dynamics

Protein Science, Volume 35, Issue 6, June 2026.
Abstract The pathological expansion of the polyglutamine (polyQ) repeat within the first exon of huntingtin (Httex1) protein is a defining hallmark of Huntington's disease (HD). Multiple evidence supports that the membrane recruitment of Httex1 is critical for its self‐assembly and related toxicity in HD.
Tânia Sousa, Gonçalo Damas, Ana Coutinho, Nuno Bernardes, Ana Azevedo, Manuel Prieto, Ana M. Melo   +6 more
wiley   +1 more source

Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET [PDF]

Nature, 2019
The HIV-1 envelope glycoprotein (Env) trimer mediates cell entry and is conformationally dynamic1-8. Imaging by single-molecule fluorescence resonance energy transfer (smFRET) has revealed that, on the surface of intact virions, mature pre-fusion Env transitions from a pre-triggered conformation (state 1) through a default intermediate conformation ...
Lu, Maolin, Ma, Xiaochu, Castillo-Menendez, Luis R., Gorman, Jason, Alsahafi, Nirmin, Ermel, Utz, Terry, Daniel S., Chambers, Michael, Peng, Dongjun, Zhang, Baoshan, Zhou, Tongqing, Reichard, Nick, Wang, Kevin, Grover, Jonathan R., Carman, Brennan P., Gardner, Matthew R., Nikic-Spiegel, Ivana, Sugawara, Akihiro, Arthos, James, Lemke, Edward A., Smith, Amos B., Farzan, Michael, Abrams, Cameron, Munro, James B., McDermott, Adrian B., Finzi, Andrés, Kwong, Peter D., Blanchard, Scott C., Sodroski, Joseph G., Mothes, Walther   +29 more
openaire   +2 more sources

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, Volume 600, Issue 9, Page 1373-1385, May 2026.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

smFRET reveals DHX36 repetitive binding,not unfolding,of G-quadruplexes

, 2021
Our data challenge Chen et al.'s interpretation of smFRET results, i.e. the repetitive unfolding of G4 with one-base translocations. We believe that the observed oscillatory curve represents the alternate binding of DHX36 to the 3' and 5'G-tetrad of G4s, rather than the repetitive unfolding between the canonical and the transformed non-canonical G4s ...
Guo, Hai-Lei, Liu, Na-Nv, Xi, Xu-Guang
openaire   +2 more sources

AAA+ protein unfoldases—the Moirai of the proteome

FEBS Letters, Volume 600, Issue 10, Page 1444-1474, May 2026.
AAA+ unfoldases are essential molecular motors that power protein degradation and disaggregation. This review integrates recent cryo‐electron microscopy (cryo‐EM) structures and single‐molecule biophysical data to reconcile competing models of substrate translocation.
Stavros Azinas, Marta Carroni
wiley   +1 more source

Advances in Double‐Stranded DNA Targeting Technologies

Exploration, Volume 6, Issue 1, February 2026.
Double‐stranded DNA (dsDNA) plays a crucial role in genetic information storage and disease management, but its inherent stability limits access to internal bases. To overcome this challenge, various high‐specificity molecular targeting technologies have been developed to destabilize the DNA structure.
Zuhao Shen, Yiqun Liu, Yingjie Hao, Yifan Bo, Xiaochuan Dai, Shihui Wang, Tian Xia, Xin Su, Huiyu Liu   +8 more
wiley   +1 more source