Results 71 to 80 of about 4,596 (191)
Chemical and Biological Determinants of PEG‐Lipid Shedding From Therapeutic Nanoparticles
Small, Volume 22, Issue 58, 16 October 2026.PEG‐lipids play a central role in governing lipid nanoparticle stability, targeting, and function. However, PEG‐lipids can “shed” from the nanoparticle surface in biological environments, with the extent and kinetics of shedding influenced by chemical and biological factors.
Ethan P. Cisneros +2 more
wiley +1 more source
Nanorulers for Super‐Resolution Microscopy
ChemBioChem, Volume 27, Issue 18, 28 September 2026.Nanorulers serve as essential calibration standards to validate optical super‐resolution microscopy down to the Ångström scale. Complementary DNA‐, protein‐, and virus‐based scaffolds provide unique strategies to benchmark spatial resolution, evaluate quantitative labeling efficiency, and mini‐mize linkage errors.
Gerti Beliu +5 more
wiley +1 more source
Advanced Science, Volume 13, Issue 52, 18 September 2026.Employing a digital single‐molecule activity tracker (dSMAT), this research demonstrates that high‐photon‐flux irradiation drives progressive oxidative scarring in polymerases. Unlike simple thermal denaturation, real‐time kinetic tracking dynamically visualizes enzymes degrading into multiple impaired subpopulations.
Anran Zheng +11 more
wiley +1 more source
BRET‐Based Approaches for Ion Channels: Emerging Applications and Future Directions
Archiv der Pharmazie, Volume 359, Issue 9, September 2026.Bioluminescence resonance energy transfer (BRET) is a versatile method for studying ligand–protein interactions. Its compatibility with high‐throughput screening and real‐time kinetics has made it widely used in drug discovery. Here, we summarize recent applications of BRET‐based methods for studying ligand binding to ion channels, highlighting key ...
Nadine Kock +6 more
wiley +1 more source
, 2019 The raw smFRET data of the Cterm of the Alb3 protein. One experiment contains no protein and the others are four repeats of the same experiment.Each line in each file corresponds to a photon.
Mahmoud Moradi (7824659)
core +1 more source
Nature Communications, 2021 N-Methyl-D-aspartate receptors (NMDARs) activation involves closure of the GluN1 and GluN2 subunit ligand binding domains, which is regulated allosterically by the amino-terminal domain (ATD).
Vojtech Vyklicky +3 more
doaj +1 more source
Denoise Stepwise Signals by Diffusion Model‐Based Approach
Small Methods, Volume 10, Issue 18, 23 September 2026.This work presents SSDM, a diffusion‐model‐based framework for denoising stepwise signals in single‐molecule measurements. By learning the statistical structure of state transitions and noise, SSDM reconstructs signal levels and identifies transition time points more accurately than conventional approaches, enabling robust analysis of signals across ...
Xingdi Tong, Chenyu Wen
wiley +1 more source
Measuring the Dynamics of TFIIF on RNA Polymerase II by smFRET [PDF]
Biophysical Journal, 2016 The making of mRNA transcripts in eukaryotes depends on an enzyme called RNA polymerase II. This enzyme is incapable of recognizing the promoter sequence on the DNA template until it forms a pre-initiation complex with a numerous transcription factors.
openaire +1 more source
Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
Nature Communications, 2020 Here, using cryo-EM and smFRET, Henderson et al. show how tryptophan 571 in the HIV-1 Env acts as a conformational switch during receptor-mediated viral entry and design HIV-1 Env proteins that cannot undergo conformational changes.
Rory Henderson +17 more
doaj +1 more source
Current Challenges of Transcription Compartmentalization Research
Advanced Science, Volume 13, Issue 46, 17 August 2026.Transcription factors, coactivators, and RNA polymerase II assemble into transcription compartments ranging from small, defined complexes to liquid‐like condensates. This review unifies these seemingly competing descriptions along a single continuum and asks what these compartments have been shown to do, and what they have not, revealing that the most ...
Thomas Quail, Sina Wittmann
wiley +1 more source