Results 71 to 80 of about 4,500 (194)
Current Challenges of Transcription Compartmentalization Research
Advanced Science, Volume 13, Issue 46, 17 August 2026.Transcription factors, coactivators, and RNA polymerase II assemble into transcription compartments ranging from small, defined complexes to liquid‐like condensates. This review unifies these seemingly competing descriptions along a single continuum and asks what these compartments have been shown to do, and what they have not, revealing that the most ...
Thomas Quail, Sina Wittmann
wiley +1 more source
Measuring the Dynamics of TFIIF on RNA Polymerase II by smFRET [PDF]
Biophysical Journal, 2016 The making of mRNA transcripts in eukaryotes depends on an enzyme called RNA polymerase II. This enzyme is incapable of recognizing the promoter sequence on the DNA template until it forms a pre-initiation complex with a numerous transcription factors.
openaire +1 more source
The smfBox is an open-source platform for single-molecule FRET
Nature Communications, 2020 Broad uptake of smFRET has been hindered by high instrument costs and a lack of open-source hardware and acquisition software. Here, the authors present the smfBox, a cost-effective open-source platform capable of measuring precise FRET efficiencies ...
Benjamin Ambrose +10 more
doaj +1 more source
Nature Communications, 2021 N-Methyl-D-aspartate receptors (NMDARs) activation involves closure of the GluN1 and GluN2 subunit ligand binding domains, which is regulated allosterically by the amino-terminal domain (ATD).
Vojtech Vyklicky +3 more
doaj +1 more source
Single-Molecule FRET Imaging of Virus Spike–Host Interactions
Viruses, 2021 As a major surface glycoprotein of enveloped viruses, the virus spike protein is a primary target for vaccines and anti-viral treatments. Current vaccines aiming at controlling the COVID-19 pandemic are mostly directed against the SARS-CoV-2 spike ...
Maolin Lu
doaj +1 more source
AI‐Physics‐Experiment Trinity for Integrated Protein Dynamics Modeling
Advanced Science, Volume 13, Issue 41, 22 July 2026.This review unites experiments, physics‐based simulations, and AI as a synergistic triad for protein dynamics modeling. It highlights integrative strategies, resolves sampling and forcefield bottlenecks, and outlines challenges and future directions for accurate, interpretable conformational ensemble prediction.
Chen Shi +4 more
wiley +1 more source
, 2019 The raw smFRET data of the Cterm of the Alb3 protein. One experiment contains no protein and the others are four repeats of the same experiment.Each line in each file corresponds to a photon.
Mahmoud Moradi (7824659)
core +1 more source
Disruption of the HIV-1 Envelope allosteric network blocks CD4-induced rearrangements
Nature Communications, 2020 Here, using cryo-EM and smFRET, Henderson et al. show how tryptophan 571 in the HIV-1 Env acts as a conformational switch during receptor-mediated viral entry and design HIV-1 Env proteins that cannot undergo conformational changes.
Rory Henderson +17 more
doaj +1 more source
ChemBioChem, Volume 27, Issue 11, 15 June 2026.We present a rational purification strategy for stochastic donor–acceptor labeling of proteins for Förster resonance energy transfer (FRET) studies. By characterizing interactions of hydrolyzed fluorophore–maleimides with anion exchange matrices, we establish predictive rules for purification design.
Nicolas D. Wendler, Thorben Cordes
wiley +1 more source
smFRET imaging assay for visualization of EBOV GP conformational changes related to membrane fusion.
, 2020 (A) Experimental setup of smFRET imaging assay. (B) Structural model of the prefusion GPΔmuc trimer with one protomer labeled with donor and acceptor fluorophores (model based on PDB 5JQ3; Materials and methods). Unlabeled protomers within the trimer are
Fernando Senjobe (8431518) +7 more
core +1 more source