Results 61 to 70 of about 4,596 (191)
Small, EarlyView.This mini review highlights how machine learning can accelerate the design of programmable nanosystems. It summarizes data‐driven approaches for predicting liposome properties, guiding DNA origami design and image analysis, and optimizing emerging liposome‐DNA hybrid platforms.
Daniel Q. Zhang, Matthew A. B. Baker
wiley +1 more source
Example datasets of confocal smFRET experiments in Photon-HDF5 format
, 2016 This fileset contains freely-diffusing single-molecule FRET datasets on the Photon-HDF5 file format (http://photon-hdf5.org/). These files are also used as demo datasets for the FRETBursts burst analysis software (http://tritemio.github.io/FRETBursts ...
Sangyoon Chung (561848) +1 more
core +1 more source
smFRET imaging assay for visualization of EBOV GP conformational changes related to membrane fusion.
, 2020 (A) Experimental setup of smFRET imaging assay. (B) Structural model of the prefusion GPΔmuc trimer with one protomer labeled with donor and acceptor fluorophores (model based on PDB 5JQ3; Materials and methods). Unlabeled protomers within the trimer are
Fernando Senjobe (8431518) +7 more
core +1 more source
High-Resolution smFRET in a Microfluidic Gas Exchange Platform [PDF]
Biophysical Journal, 2010 Single-molecule Forster Resonance Energy Transfer (smFRET) is a powerful technique providing new insights in the physics and chemistry of bio-molecules. The smFRET signal quality depends on the photon-flux that can be harvested from the fluorescent dyes.
Gambin, Yann +4 more
openaire +1 more source
Simulation of freely-diffusing smFRET data of a static mixture of 2 populations
, 2016 <p>Simulation data created with PyBroMo smFRET simulation software.</p ...
Ingargiola, Antonino (5287081) +1 more
core +1 more source
8-spot smFRET measurements on 6 dsDNA samples
, 2016 Single-molecule FRET measurements on 5 doubly-labeled and 1 donor-only dsDNA sample performed on a 8-spot smFRET system. An additional buffer-only measurement (TE50) is included for completeness.Copyright 2016 The Regents of the University of ...
Xavier Michalet (2570443) +1 more
core +1 more source
Automated smFRET microscope for the quantification of label-free DNA oligos
Biomedical Optics Express, 2019 Single-molecule FRET (smFRET) spectroscopy is a powerful tool for studying inhomogeneous dynamics in biological systems. However, because of the intrinsic variations that accompany the sample sizes, massive data sets are essential to extract statistically reliable information.
Ran Lin, Yuhong Wang
openaire +2 more sources
Force and Fluorescence Experiments in a Hybrid smFRET Magnetic Tweezer [PDF]
Biophysical Journal, 2013 Single-molecule FRET has become a powerful tool to study conformational changes in cellular machines and many other biomolecules. Labeled with fluorophores at specific positions, distance changes can be tracked with high temporal and spatial resolution.
Swoboda, Marko +4 more
openaire +1 more source
CXMS and smFRET restraints for the ensemble structure determination of Lys63-linked diubiquitin
, 2017 <p>Lys63-linked diubiquitin dynamically interconverts among three conformational states. The ensemble structures of this multi-domain protein can be determined through conjoined refinement against single-molecule fluorescence resonance energy ...
Tang Chun, LIu Zhu
core +1 more source
Single-molecule analysis reveals the kinetics and physiological relevance of MutL-ssDNA binding. [PDF]
PLoS ONE, 2010 DNA binding by MutL homologs (MLH/PMS) during mismatch repair (MMR) has been considered based on biochemical and genetic studies. Bulk studies with MutL and its yeast homologs Mlh1-Pms1 have suggested an integral role for a single-stranded DNA (ssDNA ...
Jonghyun Park +5 more
doaj +1 more source