Results 61 to 70 of about 4,500 (194)
smFRET Detection of Cis and Trans DNA Interactions by the BfiI Restriction Endonuclease
The Journal of Physical Chemistry B, 2023 Protein-DNA interactions are fundamental to many biological processes. Proteins must find their target site on a DNA molecule to perform their function, and mechanisms for target search differ across proteins. Especially challenging phenomena to monitor and understand are transient binding events that occur across two DNA target sites, whether ...
Šaru̅nė Ivanovaitė +9 more
openaire +2 more sources
Chemical and Biological Determinants of PEG‐Lipid Shedding From Therapeutic Nanoparticles
Small, EarlyView.PEG‐lipids play a central role in governing lipid nanoparticle stability, targeting, and function. However, PEG‐lipids can “shed” from the nanoparticle surface in biological environments, with the extent and kinetics of shedding influenced by chemical and biological factors.
Ethan P. Cisneros +2 more
wiley +1 more source
Denoise Stepwise Signals by Diffusion Model‐Based Approach
Small Methods, EarlyView.This work presents SSDM, a diffusion‐model‐based framework for denoising stepwise signals in single‐molecule measurements. By learning the statistical structure of state transitions and noise, SSDM reconstructs signal levels and identifies transition time points more accurately than conventional approaches, enabling robust analysis of signals across ...
Xingdi Tong, Chenyu Wen
wiley +1 more source
Single-molecule analysis reveals the kinetics and physiological relevance of MutL-ssDNA binding. [PDF]
PLoS ONE, 2010 DNA binding by MutL homologs (MLH/PMS) during mismatch repair (MMR) has been considered based on biochemical and genetic studies. Bulk studies with MutL and its yeast homologs Mlh1-Pms1 have suggested an integral role for a single-stranded DNA (ssDNA ...
Jonghyun Park +5 more
doaj +1 more source
Conformational ensembles of the disordered 4E‐BP2:eIF4E complex restrained by smFRET experiments
Protein Science, Volume 35, Issue 10, October 2026.Abstract Eukaryotic cap‐dependent translation initiation is regulated by binding of the predominantly folded eukaryotic initiation factor 4E (eIF4E) to the intrinsically disordered eIF4E binding proteins (4E‐BPs). Here, we report full‐length atomistic conformational ensembles generated by IDPConformerGenerator and optimized by X‐EISDv2 workflow for ...
Spencer Smyth +5 more
wiley +1 more source
Automated smFRET microscope for the quantification of label-free DNA oligos
Biomedical Optics Express, 2019 Single-molecule FRET (smFRET) spectroscopy is a powerful tool for studying inhomogeneous dynamics in biological systems. However, because of the intrinsic variations that accompany the sample sizes, massive data sets are essential to extract statistically reliable information.
Ran Lin, Yuhong Wang
openaire +2 more sources
8-spot smFRET measurements on 6 dsDNA samples
, 2016 Single-molecule FRET measurements on 5 doubly-labeled and 1 donor-only dsDNA sample performed on a 8-spot smFRET system. An additional buffer-only measurement (TE50) is included for completeness.Copyright 2016 The Regents of the University of ...
Xavier Michalet (2570443) +1 more
core +1 more source
BRET‐Based Approaches for Ion Channels: Emerging Applications and Future Directions
Archiv der Pharmazie, Volume 359, Issue 9, September 2026.Bioluminescence resonance energy transfer (BRET) is a versatile method for studying ligand–protein interactions. Its compatibility with high‐throughput screening and real‐time kinetics has made it widely used in drug discovery. Here, we summarize recent applications of BRET‐based methods for studying ligand binding to ion channels, highlighting key ...
Nadine Kock +6 more
wiley +1 more source
smFRET studies of the ‘encounter’ complexes and subsequent intermediate states that regulate the selectivity of ligand binding [PDF]
, 2014 The selectivity with which a biomolecule can bind its cognate ligand when confronted by the vast array of structurally similar, competing ligands that are present in the cell underlies the fidelity of some of the most fundamental processes in biology ...
Ruben L. Gonzalez +3 more
core +1 more source
Crowding-induced morphological changes in synthetic lipid vesicles determined using smFRET
Frontiers in Bioengineering and Biotechnology, 2022 Lipid vesicles are valuable mesoscale molecular confinement vessels for studying membrane mechanics and lipid–protein interactions, and they have found utility among bio-inspired technologies, including drug delivery vehicles.
Steven D. Quinn +9 more
doaj +1 more source