Results 41 to 50 of about 4,596 (191)
Angewandte Chemie, Volume 138, Issue 42, 12 October 2026.Modifying the conformational ensemble of “molten‐globule” protein by the site‐specific incorporation of stereoisomeric 4‐fluoroproline residues modulated its binding affinity and aggregation behavior. 19F NMR spectroscopy enabled the detection of key interactions responsible for structural and dynamic changes. ABSTRACT Recently, the application of deep
Abir Ben Bouzayene +5 more
wiley +2 more sources
Bio-Protocol, 2018 Single-molecule FRET (smFRET) is a powerful tool to investigate molecular structures and conformational changes of biological molecules. The technique requires protein samples that are site-specifically equipped with a pair of donor and acceptor ...
Mayuri Sadoine +3 more
doaj +1 more source
Nature Communications, 2023 The VTC complex generate eukaryotic polyphosphate (polyP). Authors here reveal how the signal-activated VTC synthesize cytosolic polyP and transport polyP across vacuolar membrane into the lumen, by using cryo-EM, in-cell NMR, and smFRET analyses.
Zeyuan Guan +20 more
doaj +1 more source
Time-resolved burst variance analysis
Biophysical Reports, 2023 Quantifying biomolecular dynamics has become a major task of single-molecule fluorescence spectroscopy methods. In single-molecule Förster resonance energy transfer (smFRET), kinetic information is extracted from the stream of photons emitted by attached
Ivan Terterov +3 more
doaj +1 more source
Observation of long-range tertiary interactions during ligand binding by the TPP riboswitch aptamer
eLife, 2015 The thiamine pyrophosphate (TPP) riboswitch is a cis-regulatory element in mRNA that modifies gene expression in response to TPP concentration. Its specificity is dependent upon conformational changes that take place within its aptamer domain.
Van K Duesterberg +4 more
doaj +1 more source
Effects of DNA looping behavior using smFRET
, 2023 This study developed a single-molecule-based assay to track the looping (circularization) of individual double-stranded DNA (dsDNA) molecules in real time. DNA encodes our genetic information through a unique combination of four nucleotides: Adenine (A), Thymine (T), Cytosine (C), and Guanine (G).
West, Natalie, Cannon, Brian
openaire +1 more source
, 2015 An example smFRET us-ALEX measurement in Photon-HDF5 format.
Sangyoon Chung (561848) +1 more
core +1 more source
Nature Communications, 2023 Growing RNAs fold differently as they are transcribed, which modulates their finally adopted structures. Riboswitches regulate gene expression by structural change, which are sensitive to co-transcriptionally structural biology.
Yanyan Xue +5 more
doaj +1 more source
smFRET Detection of Cis and Trans DNA Interactions by the BfiI Restriction Endonuclease
The Journal of Physical Chemistry B, 2023 Protein-DNA interactions are fundamental to many biological processes. Proteins must find their target site on a DNA molecule to perform their function, and mechanisms for target search differ across proteins. Especially challenging phenomena to monitor and understand are transient binding events that occur across two DNA target sites, whether ...
Šaru̅nė Ivanovaitė +9 more
openaire +2 more sources
Nature Communications, 2023 Single-molecule experiments have changed the way we explore the physical world, yet data analysis remains time-consuming and prone to human bias. Here, we introduce Deep-LASI (Deep-Learning Assisted Single-molecule Imaging analysis), a software suite ...
Simon Wanninger +6 more
doaj +1 more source