Results 41 to 50 of about 4,500 (194)
Bio-Protocol, 2018 Single-molecule FRET (smFRET) is a powerful tool to investigate molecular structures and conformational changes of biological molecules. The technique requires protein samples that are site-specifically equipped with a pair of donor and acceptor ...
Mayuri Sadoine +3 more
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Nature Communications, 2022 Slippery sequences in mRNA can cause the ribosome to change its reading frame. Using smFRET, Poulis et al. show how reversible fluctuations of peptidyl-tRNA slow down translocation, alter ribosome dynamics, and favor spontaneous ribosome frameshifting.
Panagiotis Poulis +3 more
doaj +1 more source
Effects of DNA looping behavior using smFRET
, 2023 This study developed a single-molecule-based assay to track the looping (circularization) of individual double-stranded DNA (dsDNA) molecules in real time. DNA encodes our genetic information through a unique combination of four nucleotides: Adenine (A), Thymine (T), Cytosine (C), and Guanine (G).
West, Natalie, Cannon, Brian
openaire +1 more source
Nature Communications, 2023 Single-molecule experiments have changed the way we explore the physical world, yet data analysis remains time-consuming and prone to human bias. Here, we introduce Deep-LASI (Deep-Learning Assisted Single-molecule Imaging analysis), a software suite ...
Simon Wanninger +6 more
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Observation of long-range tertiary interactions during ligand binding by the TPP riboswitch aptamer
eLife, 2015 The thiamine pyrophosphate (TPP) riboswitch is a cis-regulatory element in mRNA that modifies gene expression in response to TPP concentration. Its specificity is dependent upon conformational changes that take place within its aptamer domain.
Van K Duesterberg +4 more
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Nature Communications, 2023 The VTC complex generate eukaryotic polyphosphate (polyP). Authors here reveal how the signal-activated VTC synthesize cytosolic polyP and transport polyP across vacuolar membrane into the lumen, by using cryo-EM, in-cell NMR, and smFRET analyses.
Zeyuan Guan +20 more
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, 2015 An example smFRET us-ALEX measurement in Photon-HDF5 format.
Sangyoon Chung (561848) +1 more
core +1 more source
Nature Communications, 2021 Here, the authors use smFRET to assess the structural dynamics of metabotropic glutamate receptor mGlu2 and show that a positive allosteric modulator or the Gi protein stabilize mGlu2 in the glutamate-induced active state, leading to the full activation ...
Anne-Marinette Cao +5 more
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High-Resolution smFRET in a Microfluidic Gas Exchange Platform [PDF]
Biophysical Journal, 2010 Single-molecule Forster Resonance Energy Transfer (smFRET) is a powerful technique providing new insights in the physics and chemistry of bio-molecules. The smFRET signal quality depends on the photon-flux that can be harvested from the fluorescent dyes.
Gambin, Yann +4 more
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Example datasets of confocal smFRET experiments in Photon-HDF5 format
, 2016 This fileset contains freely-diffusing single-molecule FRET datasets on the Photon-HDF5 file format (http://photon-hdf5.org/). These files are also used as demo datasets for the FRETBursts burst analysis software (http://tritemio.github.io/FRETBursts ...
Sangyoon Chung (561848) +1 more
core +1 more source