Results 21 to 30 of about 4,500 (194)
Structural Dynamics of Glutamate Signaling Systems by smFRET [PDF]
Biophysical Journal, 2020 Single-molecule Förster resonance energy transfer (smFRET) is a powerful technique for investigating the structural dynamics of biological macromolecules. smFRET reveals the conformational landscape and dynamic changes of proteins by building on the static structures found using cryo-electron microscopy, x-ray crystallography, and other methods ...
Ryan J. Durham +3 more
openaire +2 more sources
Single-Molecule Forster Resonance Energy Transfer With a Minimalistic 3D-Printed Setup and Dyes in the Blue-Green Spectral Region. [PDF]
ChemphyschemSingle‐molecule FRET enables nanometer‐scale insights into biomolecular structure and dynamics but often requires complex instrumentation. We present the FRET‐Brick, a minimalistic, 3D‐printed microspectroscopy platform using 488 nm continuous‐wave excitation and PMT detection.
Moya Muñoz GG +6 more
europepmc +2 more sources
Simulations of camera-based single-molecule fluorescence experiments. [PDF]
PLoS ONE, 2018 Single-molecule microscopy has become a widely used technique in (bio)physics and (bio)chemistry. A popular implementation is single-molecule Förster Resonance Energy Transfer (smFRET), for which total internal reflection fluorescence microscopy is ...
Richard Börner +5 more
doaj +1 more source
Conformational Dynamics of DNA Polymerases Revealed at the Single-Molecule Level
Frontiers in Molecular Biosciences, 2022 DNA polymerases are intrinsically dynamic macromolecular machines. The purpose of this review is to describe the single-molecule Förster resonance energy transfer (smFRET) methods that are used to probe the conformational dynamics of DNA polymerases ...
David P. Millar
doaj +1 more source
Single-photon smFRET. III. Application to pulsed illumination
Biophysical Reports, 2022 Abstract Förster resonance energy transfer (FRET) using pulsed illumination has been pivotal in leveraging lifetime information in FRET analysis. However, there remain major challenges in quantitative single photon, single molecule FRET (smFRET) data analysis under pulsed illumination including: 1) simultaneously deducing kinetics and ...
Matthew Safar +7 more
openaire +2 more sources
Methods for automating the analysis of live-cell single-molecule FRET data
Frontiers in Cell and Developmental Biology, 2023 Single-molecule FRET (smFRET) is a powerful imaging platform capable of revealing dynamic changes in the conformation and proximity of biological molecules.
Jozsef Meszaros +11 more
doaj +1 more source
Single Molecule FRET: A Powerful Tool to Study Intrinsically Disordered Proteins
Biomolecules, 2018 Intrinsically disordered proteins (IDPs) are often modeled using ideas from polymer physics that suggest they smoothly explore all corners of configuration space.
Sharonda J. LeBlanc +2 more
doaj +1 more source
Molecules, 2020 RNA-protein complexes (RNPs) are essential components in a variety of cellular processes, and oftentimes exhibit complex structures and show mechanisms that are highly dynamic in conformation and structure.
Nathalie Meiser +2 more
doaj +1 more source
BOBA FRET: bootstrap-based analysis of single-molecule FRET data. [PDF]
PLoS ONE, 2013 Time-binned single-molecule Förster resonance energy transfer (smFRET) experiments with surface-tethered nucleic acids or proteins permit to follow folding and catalysis of single molecules in real-time. Due to the intrinsically low signal-to-noise ratio
Sebastian L B König +6 more
doaj +1 more source
eLife, 2020 Single-molecule Förster Resonance energy transfer (smFRET) is an adaptable method for studying the structure and dynamics of biomolecules. The development of high throughput methodologies and the growth of commercial instrumentation have outpaced the ...
Johannes Thomsen +8 more
doaj +1 more source