Results 51 to 60 of about 4,500 (194)
Biomolecules, 2022 For single-molecule studies in solution, very small concentrations of dye-labelled molecules are employed in order to achieve single-molecule sensitivity.
Olessya Yukhnovets +4 more
doaj +1 more source
Multiperspective smFRET reveals rate-determining late intermediates of ribosomal translocation [PDF]
Nature Structural & Molecular Biology, 2016 Directional translocation of the ribosome through the mRNA open reading frame is a critical determinant of translational fidelity. This process entails a complex interplay of large-scale conformational changes within the actively translating particle, which together coordinate the movement of tRNA and mRNA substrates with respect to the large and small
Wasserman, Michael R. +3 more
openaire +2 more sources
Advanced Science, EarlyView.Employing a digital single‐molecule activity tracker (dSMAT), this research demonstrates that high‐photon‐flux irradiation drives progressive oxidative scarring in polymerases. Unlike simple thermal denaturation, real‐time kinetic tracking dynamically visualizes enzymes degrading into multiple impaired subpopulations.
Anran Zheng +11 more
wiley +1 more source
HT-smFRET datasets and analysis files
, 2019 Single-molecule FRET datasets and analysis files associated with the article "High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins" by M.
Eitan Lerner (3697078) +4 more
core +1 more source
, 2023 Las enzimas cumplen una función muy importante como catalizadores, acelerando las reacciones químicas. Por ello, son fundamentales en los procesos bioquímicos.
Alzate Vargas, Sebastian
core +1 more source
Graphical models for inferring single molecule dynamics
BMC Bioinformatics, 2010 Background The recent explosion of experimental techniques in single molecule biophysics has generated a variety of novel time series data requiring equally novel computational tools for analysis and inference. This article describes in general terms how
Gonzalez Ruben L +4 more
doaj +1 more source
Multi-parameter photon-by-photon hidden Markov modeling
Nature Communications, 2022 In this work, the authors demonstrate the application of multi-parameter photon-by-photon hidden Markov modeling (mpH2MM) on alternating laser excitation (ALEX)-based smFRET measurements.
Paul David Harris +5 more
doaj +1 more source
Example smFRET data files in Photon-HDF5 format
, 2016 A few smFRET example data files in Photon-HDF5 format. This work was partially supported by NIH Grant R01-GM95904.
Sangyoon Chung (561848) +1 more
core +1 more source
High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins [PDF]
, 2019 Single-molecule Förster resonance energy transfer (smFRET) is a powerful technique for nanometer-scale studies of single molecules. Solution-based smFRET, in particular, can be used to study equilibrium intra- and intermolecular conformations, binding ...
Streets, Aaron +10 more
core +1 more source
Force and Fluorescence Experiments in a Hybrid smFRET Magnetic Tweezer [PDF]
Biophysical Journal, 2013 Single-molecule FRET has become a powerful tool to study conformational changes in cellular machines and many other biomolecules. Labeled with fluorophores at specific positions, distance changes can be tracked with high temporal and spatial resolution.
Swoboda, Marko +4 more
openaire +1 more source