Results 51 to 60 of about 4,596 (191)

Allosteric modulators enhance agonist efficacy by increasing the residence time of a GPCR in the active state

Nature Communications, 2021
Here, the authors use smFRET to assess the structural dynamics of metabotropic glutamate receptor mGlu2 and show that a positive allosteric modulator or the Gi protein stabilize mGlu2 in the glutamate-induced active state, leading to the full activation ...
Anne-Marinette Cao, Robert B. Quast, Fataneh Fatemi, Philippe Rondard, Jean-Philippe Pin, Emmanuel Margeat   +5 more
doaj   +1 more source

Multiperspective smFRET reveals rate-determining late intermediates of ribosomal translocation [PDF]

Nature Structural & Molecular Biology, 2016
Directional translocation of the ribosome through the mRNA open reading frame is a critical determinant of translational fidelity. This process entails a complex interplay of large-scale conformational changes within the actively translating particle, which together coordinate the movement of tRNA and mRNA substrates with respect to the large and small
Wasserman, Michael R., Alejo, Jose L., Altman, Roger B., Blanchard, Scott C.   +3 more
openaire   +2 more sources

Impact of Molecule Concentration, Diffusion Rates and Surface Passivation on Single-Molecule Fluorescence Studies in Solution

Biomolecules, 2022
For single-molecule studies in solution, very small concentrations of dye-labelled molecules are employed in order to achieve single-molecule sensitivity.
Olessya Yukhnovets, Henning Höfig, Nuno Bustorff, Alexandros Katranidis, Jörg Fitter   +4 more
doaj   +1 more source

Example smFRET data files in Photon-HDF5 format

, 2016
A few smFRET example data files in Photon-HDF5 format. This work was partially supported by NIH Grant R01-GM95904.
Sangyoon Chung (561848), Antonino Ingargiola (556170)   +1 more
core   +1 more source

Invisible but not inaccessible—Revealing transient oligomers formed by intrinsically disordered proteins with solution NMR and complementary methods

FEBS Letters, EarlyView.
Transient oligomers formed by intrinsically disordered proteins may be ‘invisible’ to direct detection yet remain accessible to solution NMR through equilibrium‐exchange measurements and pressure‐jump experiments. Complementary methods report on mass, stoichiometry, selected distance distributions, morphology, and internal packing.
Martin D. Gelenter, Ad Bax
wiley   +1 more source

How structural disorder and polyelectrolyte properties of prothymosin α enable its large interactome

FEBS Letters, EarlyView.
In this review, complexes of the fully disordered and highly negatively charged prothymosin α are collected, described, and discussed. Linked to its poly‐electrostatic character, prothymosin α engages a large interactome through multiple different binding modes ranging from fully disordered complexes to short linear motif (SLiM)‐driven interactions ...
Ketty C. Tamburrini, Freia S. Buus, Benjamin Schuler, Birthe B. Kragelund   +3 more
wiley   +1 more source

HT-smFRET datasets and analysis files

, 2019
Single-molecule FRET datasets and analysis files associated with the article "High-throughput smFRET analysis of freely diffusing nucleic acid molecules and associated proteins" by M.
Eitan Lerner (3697078), Xavier Michalet (2570443), shimon weiss (4850299), Maya Segal (4173715), Antonino Ingargiola (556170)   +4 more
core   +1 more source

Graphical models for inferring single molecule dynamics

BMC Bioinformatics, 2010
Background The recent explosion of experimental techniques in single molecule biophysics has generated a variety of novel time series data requiring equally novel computational tools for analysis and inference. This article describes in general terms how
Gonzalez Ruben L, Fei Jingyi, Hofman Jake M, Bronson Jonathan E, Wiggins Chris H   +4 more
doaj   +1 more source

Multi-parameter photon-by-photon hidden Markov modeling

Nature Communications, 2022
In this work, the authors demonstrate the application of multi-parameter photon-by-photon hidden Markov modeling (mpH2MM) on alternating laser excitation (ALEX)-based smFRET measurements.
Paul David Harris, Alessandra Narducci, Christian Gebhardt, Thorben Cordes, Shimon Weiss, Eitan Lerner   +5 more
doaj   +1 more source

Comparison of c_1/2 – values from μpsFRET and smFRET experiments.

, 2013
Comparison of c1/2 – values from μpsFRET and smFRET experiments.
Vera Böhm (404273), Aaron R. Hieb (352787), Katalin Tóth (173523), Jörg Langowski (173529), Alexander Gansen (404272)   +4 more
core   +1 more source