Results 91 to 100 of about 4,500 (194)
A DNA Scaffold Approach Facilitates 5' Labeling of the SARS-CoV-2 RNA Pseudoknot for Single-Molecule Förster Resonance Energy Transfer Investigation. [PDF]
ChemphyschemSingle-molecule Förster resonance energy transfer (smFRET) studies of highly structured RNA molecules are often frustrated by issues with efficient dye conjugation.
Graham SP +5 more
europepmc +2 more sources
Differences in Src phosphorylation of PSD‐93 and PSD‐95 drive differences in scaffolding activity
Protein Science, Volume 35, Issue 2, February 2026.Abstract Scaffold proteins contain multiple binding modules that allow for co‐localization of proteins that lack a direct interaction. Evolution resulted in different combinations of binding modules that rewired existing signal transduction pathways.
Frank A. Mindlin +3 more
wiley +1 more source
Simulation of freely-diffusing smFRET data of a static mixture of 2 populations
, 2016 <p>Simulation data created with PyBroMo smFRET simulation software.</p ...
Ingargiola, Antonino (5287081) +1 more
core +1 more source
NMR and smFRET insights into fast protein motions and their relation to function
, 2023 Proteins often undergo large-scale conformational transitions, in which secondary and tertiary structure elements (loops, helices and domains) change their structures or their positions with respect to each other. Simple considerations suggest that such dynamics should be relatively fast, while the functional cycles of many proteins are often ...
Paul Schanda, Gilad Haran
openaire +1 more source
CXMS and smFRET restraints for the ensemble structure determination of Lys63-linked diubiquitin
, 2017 <p>Lys63-linked diubiquitin dynamically interconverts among three conformational states. The ensemble structures of this multi-domain protein can be determined through conjoined refinement against single-molecule fluorescence resonance energy ...
Tang Chun, LIu Zhu
core +1 more source
Lipid Vesicle Characterization and Encapsulation for smFRET Experiments
, 2009 vii, 38 p.In eukaryotes, a protein-RNA complex called the spliceosome carries out gene splicing, a type of RNA processing. Although much is known about the spliceosome, it still remains unclear which component catalyzes the splicing reaction, RNA or ...
Black, William A.
core +1 more source
Καθαρισμός και μελέτες προετοιμασίας της πρωτεΐνης PGL-3 για smFRET [PDF]
Η διπλωματική εργασία επικεντρώνεται στον καθαρισμό και την προετοιμασία πειραμάτων για την χρήση της μεθόδου μοριακής μεταφοράς ενέργειας Förster στο επίπεδο του ενός μορίου (smFRET) της πρωτεΐνης PGL-3, μιας κρίσιμης πρωτεΐνης στη βιολογία της ...
Σιαμάνης, Τριαντάφυλλος
core
smFRET analysis reveals a conformational transition prior to nucleosome unwrapping.
, 2013 A,B) smFRET histograms of non-acetylated and H3-acetylated nucleosomes at various salt concentrations and 300 pM total nucleosome concentration. Above 300 mM NaCl, a fraction of H3-acetylated nucleosomes populates a second conformation with slightly ...
Vera Böhm (404273) +4 more
core +1 more source
CHIMIA, 2013 Single-molecule spectroscopy allows the direct observation of conformational dynamics in individual biomolecules. Here, we describe how single-molecule Förster resonance energy transfer (smFRET) reveals heterogeneous kinetics in the EBS1*/IBS1 ...
Sebastian L. B. König +2 more
doaj +1 more source
Nature CommunicationsEmbedding of cell-surface receptors into a membrane defines their dynamics but also complicates experimental characterization of their signaling complexes.
Yunqing Li +12 more
doaj +1 more source