Results 91 to 100 of about 4,596 (191)

smFRET reveals DHX36 repetitive binding,not unfolding,of G-quadruplexes

, 2021
Our data challenge Chen et al.'s interpretation of smFRET results, i.e. the repetitive unfolding of G4 with one-base translocations. We believe that the observed oscillatory curve represents the alternate binding of DHX36 to the 3' and 5'G-tetrad of G4s, rather than the repetitive unfolding between the canonical and the transformed non-canonical G4s ...
Guo, Hai-Lei, Liu, Na-Nv, Xi, Xu-Guang
openaire   +2 more sources

A DNA scaffold approach facilitates 5′ labelling of the SARS-CoV-2 RNA pseudoknot for smFRET investigation [PDF]

Single-molecule Förster resonance energy transfer (smFRET) studies of highly structured RNA molecules are often frustrated by issues with efficient dye conjugation.
BETTS, JEMMA, GRAHAM, SARAH, QUINN, STEVEN, LEAKE, MARK CHRISTIAN, Hill, Chris H, Craggs, Timothy   +5 more
core   +1 more source

Modelo multivariado oculto de Markov expandido para capturar dinámicas de enzimas durante smFRET de libre difusión

, 2023
Las enzimas cumplen una función muy importante como catalizadores, acelerando las reacciones químicas. Por ello, son fundamentales en los procesos bioquímicos.
Alzate Vargas, Sebastian
core  

smFRET analysis reveals a conformational transition prior to nucleosome unwrapping.

, 2013
A,B) smFRET histograms of non-acetylated and H3-acetylated nucleosomes at various salt concentrations and 300 pM total nucleosome concentration. Above 300 mM NaCl, a fraction of H3-acetylated nucleosomes populates a second conformation with slightly ...
Vera Böhm (404273), Aaron R. Hieb (352787), Katalin Tóth (173523), Jörg Langowski (173529), Alexander Gansen (404272)   +4 more
core   +1 more source

Extending Single-Molecule Förster Resonance Energy Transfer (FRET) Range beyond 10 Nanometers in Zero-Mode Waveguides

, 2019
Single-molecule Förster resonance energy transfer (smFRET) is widely used to monitor conformations and interaction dynamics at the molecular level. However, conventional smFRET measurements are ineffective at donor–acceptor distances exceeding 10 nm ...
Jérôme Wenger (1535314), Mikhail Baibakov (6939785), Mikhail Baibakov, Julien Lumeau, Satyajit Patra, Satyajit Patra (1375992), Julien Lumeau (2880206), Jean-Benoît Claude (3862351), Jean-Benoît Claude, Antonin Moreau (6939788), Antonin Moreau, Jérôme Wenger   +11 more
core   +2 more sources

smFRET and biochemical data for the study of PPR binding to single-stranded RNA

<p>Dataset contains (predominantly) pre-processed data for single-molecule FRET (smFRET) and other biochemical data (e.g., SDS-PAGE gels, surface plasmon resonance experiments, FPLC chromatograms).
Marzano, Nicholas
core   +1 more source

Kinetic Subpopulations Detected by Single-molecule Spectroscopy: Fundamental Property of Functional Nucleic Acids or Experimental Artefact?

CHIMIA, 2013
Single-molecule spectroscopy allows the direct observation of conformational dynamics in individual biomolecules. Here, we describe how single-molecule Förster resonance energy transfer (smFRET) reveals heterogeneous kinetics in the EBS1*/IBS1 ...
Sebastian L. B. König, Danny Kowerko, Roland K. O. Sigel   +2 more
doaj   +1 more source

Single Viruses on the Fluorescence Microscope: Imaging Molecular Mobility, Interactions and Structure Sheds New Light on Viral Replication

Viruses, 2018
Viruses are simple agents exhibiting complex reproductive mechanisms. Decades of research have provided crucial basic insights, antiviral medication and moderately successful gene therapy trials. The most infectious viral particle is, however, not always
Nagma Parveen, Doortje Borrenberghs, Susana Rocha, Jelle Hendrix   +3 more
doaj   +1 more source

Single-molecule imaging and molecular dynamics simulations reveal early activation of the MET receptor in cells

Nature Communications
Embedding of cell-surface receptors into a membrane defines their dynamics but also complicates experimental characterization of their signaling complexes.
Yunqing Li, Serena M. Arghittu, Marina S. Dietz, Gabriel J. Hella, Daniel Haße, Davide M. Ferraris, Petra Freund, Hans-Dieter Barth, Luisa Iamele, Hugo de Jonge, Hartmut H. Niemann, Roberto Covino, Mike Heilemann   +12 more
doaj   +1 more source

An experiment-informed hierarchical Dirichlet process-hidden Markov model to analyze surface-immobilized smFRET data [PDF]

Experiments using single-molecule Forster resonance energy transfer (smFRET) techniques have revolutionized the study of conformational states in biological molecules such as intrinsically disordered proteins. Analysis of smFRET data often relies on Hid-
Hidalgo Vargas, Jared N.
core