Results 191 to 200 of about 6,254 (216)
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Stochastic Langevin simulations for time-resolved confocal smFRET experiments
Biophysical JournalDavid Frost, Keisha Cook, Hugo Sanabria
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Ribosome Structure and Dynamics by smFRET Microscopy
2014Composed of both RNA and protein components, the ribosome is one of the largest macromolecular machines in life responsible for the production of all protein. Interestingly, the major catalytic center of the ribosome (the peptidyl transferase center) and much of the binding regions for both mRNA and tRNA are composed of RNA making the ribosome one of ...
Bassem, Shebl +2 more
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Integrating Prism‐based TIRF with Confocal Microscopy: An Economical Approach for smFRET Experiments
Current ProtocolsTotal internal reflection fluorescence (TIRF) microscopy enables the observation of complex bioassemblies and macromolecular dynamics in high spatial‐temporal resolution at the single‐molecule level in real time.
P. Agarwala, Dibyendu K. Sasmal
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Journal of Physical Chemistry B, 2021
Single-molecule fluorescence resonance energy transfer (smFRET) experiments permit detailed examination of microscopic dynamics. However, kinetic rate constants determined by smFRET are susceptible to systematic underestimation when the rate constants ...
D. Nicholson, D. Nesbitt
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Single-molecule fluorescence resonance energy transfer (smFRET) experiments permit detailed examination of microscopic dynamics. However, kinetic rate constants determined by smFRET are susceptible to systematic underestimation when the rate constants ...
D. Nicholson, D. Nesbitt
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smFRET experiments of the RNA polymerase II transcription initiation complex
Methods, 2017Single-molecule fluorescence and in particular single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful tool to provide real-time information on the dynamic architecture of large macromolecular structures such as eukaryotic transcription initiation complexes.
Nicole, Malkusch +4 more
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Cotranslational Incorporation into Proteins of a Fluorophore Suitable for smFRET Studies
ACS Synthetic Biology, 2018Single-molecule FRET (smFRET) is a powerful tool to investigate conformational changes of biological molecules. In general, smFRET studies require protein samples that are site-specifically double-labeled with a pair of donor and acceptor fluorophores.
Mayuri Sadoine +4 more
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Probing Dynamic Assembly and Disassembly of Rad51 Tuned by Srs2 Using smFRET
2018The integrity of DNA is critical for sustaining the life of any living organism, as DNA is a reservoir of its genetic information. However, DNA is continuously damaged by either normal metabolic pathways or environmental insults such as ultraviolet exposure or chemicals.
Yupeng, Qiu, Hye Ran, Koh, Sua, Myong
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2018
Fӧrster-type resonance energy transfer (FRET) with fluorescence cross-correlation spectroscopy (FCCS) is a powerful combination for observing intramolecular conformational dynamics on the micro- to millisecond timescale. Owing to its sensitivity to various physical parameters, FRET-FCCS has also been used to detect the reagent effects on proteins ...
Pei, Li +3 more
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Fӧrster-type resonance energy transfer (FRET) with fluorescence cross-correlation spectroscopy (FCCS) is a powerful combination for observing intramolecular conformational dynamics on the micro- to millisecond timescale. Owing to its sensitivity to various physical parameters, FRET-FCCS has also been used to detect the reagent effects on proteins ...
Pei, Li +3 more
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Structural Dynamics of SNARE Complex Assembly in the Ribbon Synapses Observed by smFRET.
Methods in molecular biologyAlexa L. Andrzejewski +4 more
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