Results 171 to 180 of about 4,596 (191)
Some of the next articles are maybe not open access.
smFRET study of rRNA dimerization at the peptidyl transfer center
Biophysical Chemistry, 2021The ribosome is a ribozyme. At the peptidyl transfer center (PTC) of 180 nt, two loops (the A- and P- loops) bind to tRNAs and position them in close proximity for efficient peptidyl ligation. There is also a 2-fold rotational symmetry in the PTC, which suggests that the precursor of the modern ribosome possibly emerged through dimerization and gene ...
Doris, Xu, Yuhong, Wang
openaire +2 more sources
smFRET Experiments of the RNA Polymerase II Transcription Initiation Complex
Methods, 2017Single-molecule fluorescence and in particular single-molecule Förster Resonance Energy Transfer (smFRET) is a powerful tool to provide real-time information on the dynamic architecture of large macromolecular structures such as eukaryotic transcription initiation complexes.
Jens Michaelis, Tobias Eilert
exaly +3 more sources
Combined smFRET and NMR Analysis of Riboswitch Structural Dynamics
Methods, 2019Harald Schwalbe +2 more
exaly +3 more sources
smFRET-assisted RNA structure prediction
Communications in Information and SystemsSingle-molecule Förster Resonance Energy Transfer (smFRET) is a powerful biophysical technique that utilizes the distance-dependent energy transfer between donor and acceptor dyes linked to individual molecules, providing insights into molecular conformational changes and interactions at the single-molecule level.
Jun Li, Nils G. Walter, Shi-Jie Chen
openaire +2 more sources
Multi-Channel smFRET Study Reveals a Compact Conformation of EF-G on the Ribosome
International Journal of Biochemistry and Cell BiologyABSTRACT While elongation factor G (EF-G) is crucial for ribosome translocation, the role of its GTP hydrolysis remains ambiguous. EF-G’s indispensability is further exemplified by the phosphorylation of human eukaryotic elongation factor 2 (eEF2) at Thr56, which inhibits protein synthesis globally, but its exact ...
Yuhong Wang
exaly +4 more sources
40S scanning unveiled by smFRET
Biophysical Journal, 2022Jinfan Wang, Joseph D. Puglisi
openaire +1 more source
Probing Dynamic Assembly and Disassembly of Rad51 Tuned by Srs2 Using smFRET
2018The integrity of DNA is critical for sustaining the life of any living organism, as DNA is a reservoir of its genetic information. However, DNA is continuously damaged by either normal metabolic pathways or environmental insults such as ultraviolet exposure or chemicals.
Yupeng, Qiu, Hye Ran, Koh, Sua, Myong
openaire +2 more sources
Observing transmembrane conformational signaling with smFRET
Biophysical Journal, 2023Gabriela Schlau-Cohen +2 more
openaire +1 more source