Results 21 to 30 of about 6,254 (216)

Single-Molecule FRET: A Tool to Characterize DNA Nanostructures

Frontiers in Molecular Biosciences, 2022
DNA nanostructures often involve temporally evolving spatial features. Tracking these temporal behaviors in real time requires sophisticated experimental methods with sufficiently high spatial and temporal resolution.
Nibedita Pal
doaj   +1 more source

High-Throughput Fabrication of Zero-Mode Waveguide Nanoaperture Arrays with Sol-Gel Nanoimprint Lithography for Enhanced Single Molecule Fluorescence Detection. [PDF]

Small
The researchers developed a scalable and cost‐effective method to fabricate zero‐mode waveguide (ZMW) nanoapertures in metallic films using sol‐gel nanoimprint lithography and vapor‐phase etching. These nanostructures confine light to attoliter volumes, enhancing fluorescence brightness and enabling single‐molecule detection at micromolar ...
Khelidj H, Gourdin A, Ozerov I, Moreau A, Kerzabi B, Grosso D, Wenger J.   +6 more
europepmc   +2 more sources

Pathogenic mechanism of the K141E mutation in HSPB8: Insights from smFRET and simulations. [PDF]

Cell Stress Chaperones
Montepietra D, Bjarnason S, Óskarsson KR, Cecconi C, Carra S, Heidarsson PO, Brancolini G.   +6 more
europepmc   +2 more sources

Structural Dynamics of Glutamate Signaling Systems by smFRET [PDF]

Biophysical Journal, 2020
Single-molecule Förster resonance energy transfer (smFRET) is a powerful technique for investigating the structural dynamics of biological macromolecules. smFRET reveals the conformational landscape and dynamic changes of proteins by building on the static structures found using cryo-electron microscopy, x-ray crystallography, and other methods ...
Ryan J. Durham, Danielle R. Latham, Hugo Sanabria, Vasanthi Jayaraman   +3 more
openaire   +2 more sources

An automated single-molecule FRET platform for high-content, multiwell plate screening of biomolecular conformations and dynamics

Nature Communications, 2023
Single-molecule FRET (smFRET) has become a versatile tool for probing the structure and functional dynamics of biomolecular systems, and is extensively used to address questions ranging from biomolecular folding to drug discovery.
Andreas Hartmann, Koushik Sreenivasa, Mathias Schenkel, Neharika Chamachi, Philipp Schake, Georg Krainer, Michael Schlierf   +6 more
doaj   +1 more source

Heterogeneous migration routes of DNA triplet repeat slip-outs

Biophysical Reports, 2022
It is unclear how the length of a repetitive DNA tract determines the onset and progression of repeat expansion diseases, but the dynamics of secondary DNA structures formed by repeat sequences are believed to play an important role.
Simona Bianco, Tianyu Hu, Oliver Henrich, Steven W. Magennis   +3 more
doaj   +1 more source

A blind benchmark of analysis tools to infer kinetic rate constants from single-molecule FRET trajectories

Nature Communications, 2022
Single-molecule FRET (smFRET) is a versatile technique to study the dynamics and function of biomolecules since it makes nanoscale movements detectable as fluorescence signals.
Markus Götz, Anders Barth, Søren S.-R. Bohr, Richard Börner, Jixin Chen, Thorben Cordes, Dorothy A. Erie, Christian Gebhardt, Mélodie C. A. S. Hadzic, George L. Hamilton, Nikos S. Hatzakis, Thorsten Hugel, Lydia Kisley, Don C. Lamb, Carlos de Lannoy, Chelsea Mahn, Dushani Dunukara, Dick de Ridder, Hugo Sanabria, Julia Schimpf, Claus A. M. Seidel, Roland K. O. Sigel, Magnus Berg Sletfjerding, Johannes Thomsen, Leonie Vollmar, Simon Wanninger, Keith R. Weninger, Pengning Xu, Sonja Schmid   +28 more
doaj   +1 more source

Simulations of camera-based single-molecule fluorescence experiments. [PDF]

PLoS ONE, 2018
Single-molecule microscopy has become a widely used technique in (bio)physics and (bio)chemistry. A popular implementation is single-molecule Förster Resonance Energy Transfer (smFRET), for which total internal reflection fluorescence microscopy is ...
Richard Börner, Danny Kowerko, Mélodie C A S Hadzic, Sebastian L B König, Marc Ritter, Roland K O Sigel   +5 more
doaj   +1 more source

Macromolecular High-Affinity Binding Probed by Advanced Fluorescence Techniques. [PDF]

Chembiochem
For interacting biomolecules in solution, confocal fluorescence microscopy enables some very effective approaches to characterize intermolecular binding in complex sample environments and with small sample consumption. These approaches range from fluorescence‐based genetically encoded biosensors for use in living cells to the high controllability in ...
Meyer A, Schedler B, Fitter J.
europepmc   +2 more sources

Conformational Dynamics of DNA Polymerases Revealed at the Single-Molecule Level

Frontiers in Molecular Biosciences, 2022
DNA polymerases are intrinsically dynamic macromolecular machines. The purpose of this review is to describe the single-molecule Förster resonance energy transfer (smFRET) methods that are used to probe the conformational dynamics of DNA polymerases ...
David P. Millar
doaj   +1 more source