The SNARE machinery is involved in apical plasma membrane trafficking in MDCK cells. [PDF]
, 1998 We have investigated the controversial involvement of components of the SNARE (soluble N-ethyl maleimide-sensitive factor [NSF] attachment protein [SNAP] receptor) machinery in membrane traffic to the apical plasma membrane of polarized epithelial (MDCK)
Chapin, SJ +6 more
core
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
A distinct tethering step is vital for vacuole membrane fusion
eLife, 2014 Past experiments with reconstituted proteoliposomes, employing assays that infer membrane fusion from fluorescent lipid dequenching, have suggested that vacuolar SNAREs alone suffice to catalyze membrane fusion in vitro.
Michael Zick, William T Wickner
doaj +1 more source
PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines
FEBS Open Bio, EarlyView.Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart +7 more
wiley +1 more source
Golgi clustering by the deficiency of COPI-SNARE in Drosophila photoreceptors
Frontiers in Cell and Developmental BiologyA comprehensive study of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) in the fly genome by RNAi in Drosophila photoreceptors indicated that knockdown of any of the COPI-SNAREs, Syx18, Sec20, and Use1, resulted in the ...
Tatsuya Tago +6 more
doaj +1 more source
Can ecosystem-based adaptation address the adaptation needs of smallholder farmers? Insights from smallholder coffee and subsistence farmers in central America [PDF]
, 2015 In many regions, climate change is having significant adverse impacts on the agricultural production and livelihoods of smallholder farmers, with important consequences for global food security..
Alpizar, Francisco +9 more
core
Organelle size control - increasing vacuole content activates SNAREs to augment organelle volume through homotypic fusion. [PDF]
, 2016 Cells control the size of their compartments relative to cell volume, but there is also size control within each organelle. Yeast vacuoles neither burst nor do they collapse into a ruffled morphology, indicating that the volume of the organellar envelope
Desfougères, Y., Mayer, A., Neumann, H.
core +3 more sources
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Best management practices in counting urban black bears
Human-Wildlife Interactions, 2017 DNA-based capture-mark-recapture (CMR) techniques are commonly used to obtain population parameters of black bears (Ursus americanus) in rural and wildland landscapes; however, these techniques have not been implemented in urban clusters (i.e., 2,500 to ...
Jonathan L. Fusaro +4 more
doaj +1 more source
The dysbindin-containing complex (BLOC-1) in brain: developmental regulation, interaction with SNARE proteins and role in neurite outgrowth. [PDF]
, 2010 Previous studies have implicated DTNBP1 as a schizophrenia susceptibility gene and its encoded protein, dysbindin, as a potential regulator of synaptic vesicle physiology.
Chan, LN +9 more
core +2 more sources