Results 201 to 210 of about 129,104 (260)

Ethnobotanical and genetic assessments of Central American avocado landraces reveal novel sources of biodiversity and ancient migration patterns

PLANTS, PEOPLE, PLANET, EarlyView.
Avocados are a multibillion‐dollar global commodity, but their growing export market pressures local farmers to abandon local fruit tree diversity in favor of commercial cultivar production. Interviews with rural avocado farmers and households from the Southern Mexican Highlands, Pacific Coastal Honduras, and Western Nicaragua, as well as the genetic ...
Kevin Wann, Heather B. Thakar, Courtney A. Hofman, Hector Neff, Logan Kistler   +4 more
wiley   +1 more source

Multiple barriers maintain species boundaries in sympatric brood‐site deceptive Gastrodia orchids

PLANTS, PEOPLE, PLANET, EarlyView.
Societal Impact Statement Gastrodia orchids are inconspicuous forest plants that lack chlorophyll and obtain carbon from fungi. Their dull‐colored, ground‐level flowers are often hidden among leaf litter, yet they rely on small flies for pollination.
Kenji Suetsugu, Akihiro Nishimura, Shun K. Hirota, Masahito T. Kimura   +3 more
wiley   +1 more source

Detecting SARS-CoV-2 variants with SNP genotyping

, 2020
Harper H, Burridge AJ, Winfield M, Finn A, Davidson AD, Matthews D, Hutchings S, Vipond B, Jain N, Edwards KJ, The COVID-19 Genomics UK (COG-UK) consortium, Barker G.   +11 more
europepmc   +1 more source

First genome sequence of a European Alternaria brassicae isolate and genes involved in early development of alternaria leaf spot on Brassica juncea

Pest Management Science, EarlyView.
This article reports the first genome sequence of a UK Alternaria brassicae isolate. Dual RNA‐sequencing profiling of A. brassicae‐infected Brassica juncea leaves identified differentially expressed genes involved in pathogenicity and host response pathways in moderately resistant Sej‐2 (2) and moderately susceptible Pusa Jaikisan cultivars.
Kevin M. King, Graham McLaughlin, Chinthani S. Karandeni Dewage, Zedi Gao, David J. Hughes, Jonathan S. West, Henrik U. Stotz   +6 more
wiley   +1 more source

Seedling resistance and chemical defenses against Psylliodes chrysocephala: the roles of seed age and sinapinic acid in Sinapis alba and Brassica napus

Pest Management Science, EarlyView.
The cabbage stem flea beetle (Psylliodes chrysocephala) is a major pest of winter oilseed rape (Brassica napus). We tested B. napus and Sinapis alba seedlings for feeding resistance and underlying chemical defenses. S. alba was least damaged, while metabolite profiles revealed resistance‐linked compounds, highlighting breeding potential for insect ...
Daniel Rüde, Samantha M Cook, Bernd Ulber, Tobias Pöhnl, Michael Rostás   +4 more
wiley   +1 more source

Resistance of Botrytis cinerea to anilinopyrimidine fungicides: A novel ARMS‐PCR method for the detection of Bcpos5 mutations and characterization of resistance using CRISPR/Cas9 editing

Pest Management Science, EarlyView.
A TETRA‐primer amplification refractory mutation system polymerase chain reaction (T‐ARMS‐PCR) method was developed for simultaneous detection of the L412F/V and G408V mutations. L412F/V mutants were created using the CRISPR/Cas tool and no fitness cost was observed.
Georgios Sofianos, Athanasios Petmezas, Anastasios Samaras, George Karaoglanidis   +3 more
wiley   +1 more source

Introgressed Brassica napus × Sinapis alba enhance resistance to the crucifer flea beetle (Phyllotreta cruciferae)

Pest Management Science, EarlyView.
This study demonstrates that Brassica napus lines with Sinapis alba introgressions enhanced resistance against Phyllotreta cruciferae, resulting in reduced feeding damage and improved seedling physiological performance. The introgressed lines showed strong potential as genetic resources for developing more resilient and sustainable canola varieties ...
Aziz Ullah, Altaf Hussain, Vicky Roslinsky, Sally L. Vail, Boyd A. Mori   +4 more
wiley   +1 more source

Target‐site mutations and phenotypic failure in Myzus persicae against pyrethroids and carbamates in central Europe

Pest Management Science, EarlyView.
Molecular screening of 140 field‐collected Myzus persicae aphids revealed target‐site mutations L1014F, M918L, M918T, and L932F in the sodium channel and S431F in Ace2. The M918L mutation was the primary driver of high pyrethroid (cypermethrin) resistance, while S431F served as a definitive marker for carbamate (pirimicarb) resistance.
Jitka Stara, Jan Hubert, Pavla Pabiskova, Frantisek Kocourek   +3 more
wiley   +1 more source

Decreased DMI sensitivity of Plenodomus biglobosus (phoma of oilseed rape) associated with CYP51 substitution G476S

Pest Management Science, EarlyView.
Here, we report for the first time a target site mutation in the CYP51 gene of the oilseed rape phoma stem canker fungus Plenodomus biglobosus (Leptosphaeria biglobosa) that is associated with decreased sensitivity to sterol demethylation inhibitor fungicides.
Kevin M King, Liza M González‐Rodríguez, Joanna Kaczmarek, Małgorzata Jędryczka, Jonathan S West   +4 more
wiley   +1 more source

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Amplifluor-Based SNP Genotyping

2023
Amplifluor, a genotyping system used to analyze single nucleotide polymorphisms (SNPs), is supplied by Merck-Millipore. Amplifluor is based on polymerase chain reaction (PCR) with two competing allele-specific primers and a SNP specific common reverse primer.
Manmode Darpan, Mohanrao, Senapathy, Senthilvel, Yarabapani Rushwanth, Reddy, Chippa Anil, Kumar, Palchamy, Kadirvel   +4 more
openaire   +2 more sources