Results 71 to 80 of about 5,383,703 (281)
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Review of national AIDS councils in Africa: Findings from five countries
SAHARA-J, 2008 National AIDS councils (NACs) were established in many African countries to co-ordinate the multi-sectoral response to HIV/ AIDS. Their main mandate is to provide strategic leadership and co-ordinate activities geared to fight against HIV/AIDS.
Charles Hongoro +2 more
doaj +1 more source
FEBS Open Bio, EarlyView.dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon +6 more
wiley +1 more source
Metabolic syndrome, hypertension, and nervous system injury: Epidemiological correlates
Clinical and Experimental Hypertension, 2017 Metabolic syndrome (MetS) is a common and complex disorder combining hypertension, obesity, dyslipidemia, and insulin resistance. MetS represents a risk factor for changes in cognitive functions in older age, and several studies have suggested that MetS ...
Giovanna Ricci +4 more
doaj +1 more source
HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein
FEBS Open Bio, EarlyView.Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley +1 more source
Social aspects of scientific knowledge [PDF]
Synthese, 2018 From its inception in 1987 social epistemology has been divided into analytic (ASE) and critical (CSE) approaches, represented by Alvin I. Goldman and Steve Fuller, respectively. In this paper, the agendas and some basic ideas of ASE and CSE are compared and assessed by bringing into the discussion also other participants of the debates on the social ...
openaire +1 more source
FEBS Open Bio, EarlyView.A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti +3 more
wiley +1 more source
Frontiers in Public HealthBackgroundIn August 2023, Poland enacted the Act on Certain Medical Professions, introducing binding legal requirements for 15 previously unregulated health professions.
Jolanta Agnieszka Pacian +1 more
doaj +1 more source
Education and training for industrial biotechnology and engineering biology
Engineering Biology, 2019 Industrial biotechnology is focused on the production of bio-based fuels, chemicals and materials such as plastics and textiles. Engineering biology, synonymous with synthetic biology, provides a platform technology that brings an engineering approach to
Camille J. Delebecque, Jim Philp
doaj +1 more source
Anchorage‐independent and faster growth in clonal population from UV‐irradiated NER‐deficient cells
FEBS Open Bio, EarlyView.UV‐irradiated cells expressing a DDB2 mutant protein unable to interact with PCNA (DDB2PCNA‐) form clones able to grow without anchorage. Different experimental approaches reveal heterogeneity in cell cycle regulation and drug response within these clones, emphasizing the crucial role of the DDB2‐PCNA interaction in preventing cellular transformation ...
Paola Perucca +6 more
wiley +1 more source