Results 121 to 130 of about 504,419 (304)

Strengthening social innovation in Europe : journey to effective assessment and metrics.

, 2012
The Social Innovation Europe initiative (SIE) represents a major effort to build and streamline the social innovation field in Europe. The project is run by a consortium of partners including Euclid Network, the Danish Technological Institute, and the ...

core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Why human connection is the true metric of research success

FEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan, Mattias Wei Ren Kon, Xavier Coumoul, Fun Man Fung   +3 more
wiley   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

The European Research Network on Men in Europe: The Social Problem and Societal Problematisation of Men and Masculinities The social problem of men: deliverable 14: Final Network Report

Changing and improving gender relations and reducing gender inequality involves changing men as well as changing the position of women. The EU Framework 5 European Research Network on Men in Europe (2000-2003) has aimed to develop empirical, theoretical ...
Millett, Jackie, Chernova, Janna, Novikova, Irina, Muller, Ursula, Holter, Oystein Gullvag, Hearn, Jeff, Olsvik, Elvind, Ventimiglia, Carmine, Lattu, Emmi, Jacobsen, Astrid, Ferguson, Harry, Oleksy, Elzbieta H., Raynor, Alex, Tallberg, Teemu, Pitch, Tamar, Kolga, Voldemar, Pringle, Keith   +16 more
core  

Social Development, Traditional Religion Movements and Migrants in Europe [PDF]

, 2019
The subject of sociological and economic analysis is the relationship between traditional religious movements, migrants and social development in contemporary Europe.
Maksimović, Marijana, Novaković, Nada
core  

European policies for social inclusion of Roma : Catch 22?

, 2015
The article analyzes contemporary political discourses with regard to social inclusion of Roma on the basis of comparison with achievements and failures in the previous historical period of the communist rule in Eastern Europe.
Elena Marushiakova, Vesselin Popov, Marushiakova-Popova, Elena, Popov, Vesselin, Marushiakova, Elena   +4 more
core   +2 more sources

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source