Results 201 to 210 of about 125,898 (258)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Shank3B deficiency disrupts GABAergic synaptic transmission in pyramidal neurons of the medial prefrontal cortex region in autism spectrum disorder. [PDF]

Mol Brain
Yin H, Sun K, Wang C, Fan S, Wang J, He S, Lei HM, Pang S, Chen J, Zhang G.   +9 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Cacna1c deficiency in parvalbumin-expressing neurons promotes anxiety and passive stress-coping behavior. [PDF]

Sci Rep
Loganathan S, Zhao C, Deussing JM.
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Ertugliflozin improves animal behaviours associated with oxidative stress and inflammation in a BTBR <i>T</i> + <i>Itpr3tf/J</i> mouse model of autism. [PDF]

Brain Commun
Wang X, Zhao Z, Sun L, Gao C, Wang L, Mei D, Hao C, Zhao S, Yan X, Liu J, Liu L, Guo B, Zhang Y.   +12 more
europepmc   +1 more source

Conditional deletion of Cc2d1a increases irritability-like behavior by decreasing oxytocin expression via the prelimbic-hypothalamic pathway. [PDF]

J Biomed Sci
Cheng KH, Hung YC, Ling P, Hsu KS.
europepmc   +1 more source

Microglial Lyn Kinase-TRPV4 axis mediates social deficits in a maternal immune activation model. [PDF]

J Neuroinflammation
Islam R, Zhang F, Choudhary HH, Yoshida J, Thomas AJ, Sorum B, Hanafy KA.   +6 more
europepmc   +1 more source

Barriers of last mile supply chain management and reproductive, maternal, new born, and child health product availability in Ethiopia. [PDF]

PLoS One
Mulatu WN, Onyango DK, Mberesero H, Tadelle G, Emaway D, Mesfin B, Shewangizaw T, Tesfaye C, Ababu A, Bogale B, Mogesi K.   +10 more
europepmc   +1 more source