Results 71 to 80 of about 13,235 (262)

Status, identity, and language: A study of issue discussions in GitHub.

PLoS ONE, 2019
Successful open source software (OSS) projects comprise freely observable, task-oriented social networks with hundreds or thousands of participants and large amounts of (textual and technical) discussion. The sheer volume of interactions and participants
Jingxian Liao, Guowei Yang, David Kavaler, Vladimir Filkov, Prem Devanbu   +4 more
doaj   +1 more source

Una experiencia de aprendizaje con etiquetado social || A learning experience based on social tagging

Revista de Estudios e Investigación en Psicología y Educación, 2015
Se exponen aquí los resultados de una experiencia educativa mediada con TIC donde jugó un papel relevante el empleo del etiquetado social o conjuntos de etiquetas creadas por múltiples usuarios en espacios web 2.0.
Francisco José Santos-Caamaño, Mercedes González-Sanmamed   +1 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Elements of social representation theory incollaborative tagging systems

Transinformação, 2014
This article discusses the information representation process based on the Moscovici's Social Representation Theory and domain analysis in Information Science.
Patricia Zeni Marchiori, Andre Luiz Appel, Eduardo Michellotti Bettoni, Denise Fukumi Tsunoda, Frank Coelho de Alcântara   +4 more
doaj  

DC-Social Tagging Workshop

, 2009
[Extract] 1. Introduction The so-called Web 2.0 brought a new breadth to the Internet, and a social perspective that seems set to stay. Services such as LinkedIn, Hi5, and Facebook have found a place in our society. People connect to each other through common paths.
Méndez, Eva, Baptista, Ana Alice
openaire   +3 more sources

Profiling Social Networks: A Social Tagging Perspective

D-Lib Magazine, 2009
The web is rapidly becoming both more open and more social through the provision of technologies that make it easier for end users to access resources and join in social networks. Social networks have pioneered online communities, allowing users to contribute to collective knowledge by tagging online resources.
Ying Ding 0001, Elin K. Jacob, James Caverlee, Michael A. H. Fried, Zhixiong Zhang   +4 more
openaire   +2 more sources

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source