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Removal of sodium dodecyl sulfate from proteins

Analytical Biochemistry, 1975
Abstract A convenient and relatively simple electrodialysis method for the removal of sodium dodecyl sulfate (SDS) from proteins is described. Six samples can be processed simultaneously. The kinetics of removal of SDS from proteins by equilibrium dialysis and electrodialysis have been studied.
G P, Tuszynski, L, Warren
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Molecular Mobility in Solid Sodium Dodecyl Sulfate

The Journal of Physical Chemistry B, 2011
Here we report on the molecular mobility in solid sodium dodecyl sulfate (SDS), a commonly used surfactant, as measured by high-resolution neutron scattering in the temperature range 175-400 K. While the quasielastic data showed the presence of dynamical motion at and above 210 K, the fixed energy window (FEW) data indicated that the dynamics is ...
S, Mitra   +4 more
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Acid-Catalyzed Hydrolysis of Sodium Dodecyl Sulfate

Journal of Pharmaceutical Sciences, 1985
The acid-catalyzed hydrolysis of sodium dodecyl sulfate (1) and the effect of 1-dodecanol (2) on this hydrolysis were investigated. The rate of hydrolysis was followed by measuring the rate of production of HSO-4 using a pH-stat. The rate constant (kH+) below the critical micelle concentration (CMC) increased with increasing concentrations of 2, up to ...
M, Nakagaki, S, Yokoyama
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Hair protein removal by sodium dodecyl sulfate

Colloids and Surfaces B: Biointerfaces, 2005
The effect of sodium dodecyl sulfate (SDS) on protein loss was studied. Three kinds of human hair were tested by rubbing or immersion in water or immersion in SDS solution, at 25, 40 and 70 degrees C. Under friction, hair treated with SDS solution loses seven times more protein than in water, while by immersion, protein loss is roughly two times higher
Rita, de Cássia Comis Wagner   +1 more
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Removal of sodium dodecyl sulfate from proteins

Analytical Biochemistry, 1978
Effective removal of sodium dodecyl sulfate from proteins in water or sodium phosphate buffer was achieved by column chromatography using the ion-retardation resin AG11A8. An average recovery of 83% protein was obtained, while 0.1 to 1.4 moles of sodium dodecyl sulfate remained on each mole of protein.
O H, Kapp, S N, Vinogradov
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Sodium dodecyl sulfate in protein chemistry

Biosystems, 1975
This review summarizes in a brief manner the main aspects of the application of sodium dodecyl sulfate (SDS) to protein chemistry. The principal problems of SDS-polyacrylamide gel electrophoresis are described, as well as the anomalous behavior of protein-SDS complexes and the inactivation of enzymes due to variable binding of SDS to the polypeptides ...
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Thermal degradation of sodium dodecyl sulfate

Journal of Agricultural and Food Chemistry, 1984
Certaines fractions produites lors de la degradation thermique du sodium dodecyl sulfate possedent une cytotoxicite superieure a celle de l'aniline. Dans le cas du tabac, ces residus resultant de l'utilisation agricole peuvent etre soumis a de hautes temperatures lors de son utilisation chez les ...
John M. Patterson   +2 more
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Physical properties of collagen-sodium dodecyl sulfate complexes

Biochemistry, 1979
Sodium dodecyl sulfate (NaDodSO4)--polyacrylamide gel electrophoresis and gel filtration chromatography of protein--NaDodSO4 complexes are frequently used to characterize collagen-like polypeptide components in mixtures obtained from extracts of basement membranes.
J W, Freytag, M E, Noelken, B G, Hudson
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Sodium Dodecyl Sulfate Adsorbed Monolayers on Gold Electrodes

Langmuir, 2007
Self-assembled aggregates of amphiphilic surfactant molecules formed on solid surfaces are similar to biological membranes. To understand the formation mechanism of these aggregates, we have studied the formation of self-organized monolayers from low-concentration sodium dodecyl sulfate (SDS) aqueous solutions (concentration below the critical micelle ...
David M, Soares   +2 more
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Sodium Dodecyl Sulfate

2001
[151-21-3] C12H25NaO4S (MW 288.38) InChI = 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1 InChIKey = DBMJMQXJHONAFJ-UHFFFAOYSA-M (used in surfactant-based reaction media) Alternate Names: SDS; sodium lauryl sulfate; SLS.
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