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Sodium dodecyl sulfate in protein chemistry

Biosystems, 1975
This review summarizes in a brief manner the main aspects of the application of sodium dodecyl sulfate (SDS) to protein chemistry. The principal problems of SDS-polyacrylamide gel electrophoresis are described, as well as the anomalous behavior of protein-SDS complexes and the inactivation of enzymes due to variable binding of SDS to the polypeptides ...
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Thermal degradation of sodium dodecyl sulfate

Journal of Agricultural and Food Chemistry, 1984
Certaines fractions produites lors de la degradation thermique du sodium dodecyl sulfate possedent une cytotoxicite superieure a celle de l'aniline. Dans le cas du tabac, ces residus resultant de l'utilisation agricole peuvent etre soumis a de hautes temperatures lors de son utilisation chez les ...
John M. Patterson   +2 more
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Physical properties of collagen-sodium dodecyl sulfate complexes

Biochemistry, 1979
Sodium dodecyl sulfate (NaDodSO4)--polyacrylamide gel electrophoresis and gel filtration chromatography of protein--NaDodSO4 complexes are frequently used to characterize collagen-like polypeptide components in mixtures obtained from extracts of basement membranes.
J W, Freytag, M E, Noelken, B G, Hudson
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Sodium Dodecyl Sulfate Adsorbed Monolayers on Gold Electrodes

Langmuir, 2007
Self-assembled aggregates of amphiphilic surfactant molecules formed on solid surfaces are similar to biological membranes. To understand the formation mechanism of these aggregates, we have studied the formation of self-organized monolayers from low-concentration sodium dodecyl sulfate (SDS) aqueous solutions (concentration below the critical micelle ...
David M, Soares   +2 more
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Sodium Dodecyl Sulfate

2001
[151-21-3] C12H25NaO4S (MW 288.38) InChI = 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1 InChIKey = DBMJMQXJHONAFJ-UHFFFAOYSA-M (used in surfactant-based reaction media) Alternate Names: SDS; sodium lauryl sulfate; SLS.
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Gas chromatographic determination of sodium dodecyl sulfate

Analytical Biochemistry, 1974
Abstract A gas chromatographic method has been developed for the determination of sodium dodecyl sulfate. The method is sensitive, reasonably accurate, and uninfluenced by the presence of protein. The method depends upon the formation of 1-dodecanol and inorganic sulfate by acidic hydrolysis of sodium dodecyl sulfate (4 n HCl, 2 hr, 100°C).
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Stability of aprA-subtilisin in sodium dodecyl sulfate

Archives of Biochemistry and Biophysics, 1988
The effect of sodium dodecyl sulfate (SDS) on the structure and activity of aprA-subtilisin, a secreted bacterial serine protease which is 85% homologous to subtilisin BPN', was examined. The addition of SDS resulted in the slow conversion of the subtilisin from the intact protein to the completely unfolded form of the enzyme.
L O, Narhi, M, Zukowski, T, Arakawa
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Antibodies to Proteins Dissolved in Sodium Dodecyl Sulfate

The Journal of Immunology, 1974
Abstract Rabbits were injected with Drosophila embryo total nonhistone chromosomal (NHC) proteins or with bovine serum albumin, both in 0.1% sodium dodecyl sulfate (SDS). Antibodies were produced which reacted specifically with the protein injected as antigen; the antibodies were not directed against a random denatured protein-SDS ...
W E, Stumph, S C, Elgin, L, Hood
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Microcapsules based on glycinin–sodium dodecyl sulfate complex coacervation

Journal of Microencapsulation, 2004
Microcapsules processed by complex coacervation were prepared using hexadecane for the oil phase and glycinin (a soybean storage protein)-sodium dodecyl sulfate (SDS) as the main wall-forming material. The study underlines the essential role of SDS, which, by the way of [glycinin(+)-SDS(-)] insoluble complex formation, allowed the precipitation of ...
Lazko, J.   +3 more
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Ultrarapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis

2018
We describe here an ultrafast method for electrophoresing proteins on SDS-PAGE. Previously we reported a method to complete SDS-PAGE and immunoblotting in an hour, including electrophoresing proteins at 70°C in 10 min. Here we show that we can electrophorese molecular weight standards and bovine serum albumin on a 4-20% gradient gel in well under 10 ...
Rohit, Thomas, Biji T, Kurien
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