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Gas chromatographic determination of sodium dodecyl sulfate
Analytical Biochemistry, 1974Abstract A gas chromatographic method has been developed for the determination of sodium dodecyl sulfate. The method is sensitive, reasonably accurate, and uninfluenced by the presence of protein. The method depends upon the formation of 1-dodecanol and inorganic sulfate by acidic hydrolysis of sodium dodecyl sulfate (4 n HCl, 2 hr, 100°C).
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Stability of aprA-subtilisin in sodium dodecyl sulfate
Archives of Biochemistry and Biophysics, 1988The effect of sodium dodecyl sulfate (SDS) on the structure and activity of aprA-subtilisin, a secreted bacterial serine protease which is 85% homologous to subtilisin BPN', was examined. The addition of SDS resulted in the slow conversion of the subtilisin from the intact protein to the completely unfolded form of the enzyme.
L O, Narhi, M, Zukowski, T, Arakawa
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Antibodies to Proteins Dissolved in Sodium Dodecyl Sulfate
The Journal of Immunology, 1974Abstract Rabbits were injected with Drosophila embryo total nonhistone chromosomal (NHC) proteins or with bovine serum albumin, both in 0.1% sodium dodecyl sulfate (SDS). Antibodies were produced which reacted specifically with the protein injected as antigen; the antibodies were not directed against a random denatured protein-SDS ...
W E, Stumph, S C, Elgin, L, Hood
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Microcapsules based on glycinin–sodium dodecyl sulfate complex coacervation
Journal of Microencapsulation, 2004Microcapsules processed by complex coacervation were prepared using hexadecane for the oil phase and glycinin (a soybean storage protein)-sodium dodecyl sulfate (SDS) as the main wall-forming material. The study underlines the essential role of SDS, which, by the way of [glycinin(+)-SDS(-)] insoluble complex formation, allowed the precipitation of ...
Lazko, J. +3 more
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Ultrarapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis
2018We describe here an ultrafast method for electrophoresing proteins on SDS-PAGE. Previously we reported a method to complete SDS-PAGE and immunoblotting in an hour, including electrophoresing proteins at 70°C in 10 min. Here we show that we can electrophorese molecular weight standards and bovine serum albumin on a 4-20% gradient gel in well under 10 ...
Rohit, Thomas, Biji T, Kurien
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Langmuir, 1991
The interface between air and surfactant solution expands rapidly during the foam generation process. Surfactant molecules in the solution should adsorb onto the newly created surface to stabilize foam. As surfactant monomers adsorb on the surface, micelles disintegrate to provide additional monomers to adsorb on the expanding interface of foam.
Seong Geun Oh, Dinesh O. Shah
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The interface between air and surfactant solution expands rapidly during the foam generation process. Surfactant molecules in the solution should adsorb onto the newly created surface to stabilize foam. As surfactant monomers adsorb on the surface, micelles disintegrate to provide additional monomers to adsorb on the expanding interface of foam.
Seong Geun Oh, Dinesh O. Shah
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Protein contaminants of sodium dodecyl sulfate-polyacrylamide gels
Analytical Biochemistry, 1983Many artifacts and confusing results have arisen following the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis and highly sensitive detection methods such as silver staining, in vitro iodination, and immunological reactions ("Western blotting").
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New nematic lyomesophase of sodium dodecyl sulfate
The Journal of Physical Chemistry, 1987Etude par microscopie optique et par diffraction des rayons X du systeme eau/decanol/sulfate lauryl de ...
L. Q. Amaral +3 more
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Powder Diffraction, 1988
AbstractIndexed X-ray diffraction powder data for three homologous amphiphiles, sodium octyl sulfate (SOS), CH3[CH2]7 OSO3Na, sodium decyl sulfate, CH3[CH2]9OSO3Na (SDS), and sodium dodecyl sulfate CH3[CH2]11OSO3Na (SLS), are reported. Probable space groups for the three compounds are monoclinic P2[3], Pm[6], or P2/m[10].
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AbstractIndexed X-ray diffraction powder data for three homologous amphiphiles, sodium octyl sulfate (SOS), CH3[CH2]7 OSO3Na, sodium decyl sulfate, CH3[CH2]9OSO3Na (SDS), and sodium dodecyl sulfate CH3[CH2]11OSO3Na (SLS), are reported. Probable space groups for the three compounds are monoclinic P2[3], Pm[6], or P2/m[10].
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Ultrathin Horizontal Sodium Dodecyl Sulfate Zymography
Analytical Biochemistry, 1995M P, Schönermark +3 more
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