Results 71 to 80 of about 14,528,728 (295)

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

ReBEC: A Method for Capturing Experience during Software Development Projects [PDF]

, 2010
In software organizations, usual ways to capture the experience project team members acquire are based on methods such as project postmortem analysis, post-project revisions and others alike. Their main drawback is that the experience capture is done (if
Andrés Silva, Gerardo Matturro, Matturro, Gerardo, Silva Vazquez, Andrés   +3 more
core   +1 more source

Gitana: A software project inspector

Science of Computer Programming, 2018
Abstract The development of software projects entails significant implementation and collaboration activities, typically supported by tools such as issue trackers, code review tools and Version Control Systems. However, these tools only provide a partial view of the project and often lack of advanced querying mechanisms, thus hampering the analysis ...
Valerio Cosentino, Javier Luis Cánovas Izquierdo, Jordi Cabot   +2 more
openaire   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

The heterodimeric amino acid transporters (HAT) of the SLC7/SLC3 family: A structure−function relationships and relevance to human pathology

FEBS Letters, EarlyView.
Heterodimeric amino acid transporters consist of SLC7 and SLC3 family proteins arranged in a conserved structural organization. They regulate nutrient transport across cell membranes, supporting essential cellular functions. These transporters also contribute to xenobiotic/drug uptake and distribution.
Mariafrancesca Scalise, Tiziano Mazza, Raffaella Scanga, Lara Console, Michele Galluccio, Cesare Indiveri   +5 more
wiley   +1 more source

Software modernization and cloudification using the ARTIST migration methodology and framework [PDF]

, 2014
Cloud computing has leveraged new software development and provisioning approaches by changing the way computing, storage and networking resources are purchased and consumed.
Bram Pellens, Stuer, Peter, Konstanteli, Kleopatra, Juncal, Alonso, Bruneliere, Hugo, Hugo Bruneliere, Strauss, Oliver, Orue-Echevarria, Leire, Senkova, Tatiana, Andreas Menychtas, Varvarigou, Theodora, Santzaridou, Christina, Gorronogoitia, Jesus, Christina Santzaridou, Menychtas, Andreas, Pellens, Bram, Theodora Varvarigou, George Kousiouris, Tatiana Senkova, Kousiouris, George, Leire Orue-echevarria, Alonso, Juncal, Strauss, Olivier, Kleopatra Konstanteli, Peter Stuer, Juncal Alonso, Oliver Strauss   +26 more
core   +1 more source

Leucine‐rich glioma inactivated 1 (LGI1) is a ganglioside‐binding protein

FEBS Letters, EarlyView.
Neuronal hyperexcitability associated with a decrease/absence of the extracellular protein LGI1 has been suggested to be primarily due to the downregulation of Kv1 channel expression. The molecular mechanisms underlying this decrease have not yet been elucidated.
Kévin Debreux, Christian Lévêque, Fodil Azzaz, Marion Sangiardi, Sarosh R Irani, Michael Seagar, Jacques Fantini, Oussama El Far   +7 more
wiley   +1 more source

Quality-aware Rapid Software Development Project: The Q-Rapids Project [PDF]

, 2019
Software quality poses continuously new challenges in software development, including aspects related to both software development and system usage, which significantly impact the success of software systems.
Oriol Hilari, Marc, Franch Gutiérrez, Javier, López Cuesta, Lidia, Trendowicz, Adam, Rodríguez, Pilar, Martinez Fernandez, Silverio   +5 more
core   +1 more source