Results 81 to 90 of about 4,013,036 (260)

Soft motions encoded by the fold and mediated by conserved hinge sites drive V‐ATPase allosteric machinery and autoinhibition

open access: yesFEBS Letters, EarlyView.
Vacuolar H+‐ATPase (V‐ATPase) is a multisubunit rotary proton pump that acidifies intracellular compartments. Using elastic network models, we identified conserved hinge residues that govern its large‐scale functional motions. We show that intrinsically encoded ‘soft modes’ drive critical functional transitions, including rotary catalysis and ...
Diksha Parwana   +3 more
wiley   +1 more source

Optimized expression of soluble cyclomaltodextrinase of thermophilic origin in Escherichia coli by using a soluble fusion-tag and by tuning of inducer concentration

open access: yes, 2005
Cyclomaltodextrinases are multidomain and often dimeric proteins from the alpha-amylase family (glycoside hydrolase family 13) which frequently have been very difficult to express in active form in Escherichia coli.
Holst, Olle   +3 more
core   +1 more source

Modelling Animal Systems Paper: Update of the Dutch protein evaluation system for ruminants: the DVE/OEB2010 system [PDF]

open access: yes, 2011
In the current Dutch protein evaluation system (the DVE/OEB1991 system), two characteristics are calculated for each feed: true protein digested in the intestine (DVE) and the rumen degradable protein balance (OEB).
Dijkstra, J.   +6 more
core   +1 more source

Expression of Soluble c-CfTXB Protein from Chironex fleckeri Jellyfish Fused to SUMO Tag in Escherichia coli BL21 (DE3) and Evaluation of Immunogenicity in Syrian Mice [PDF]

open access: yesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd
Introduction: The box jellyfish Chironex fleckeri is a toxin-producing species whose cytotoxic and neurotoxic effects have been well demonstrated in living organisms.
Mehdi Bakhshian   +3 more
doaj  

Acclimation of Young Plants of Opuntia spp. to the Heightened Night Temperature

open access: yesHorticulturae
Increases in global temperatures, due to the climate change, are generating stress in most plant species. We hypothesize that young plants of Opuntia spp.
Claudia Reyes Buendía   +4 more
doaj   +1 more source

Effects of different nitrogen forms on the nutritional quality and physiological characteristics of Chinese chive seedlings

open access: yesPlant, Soil and Environment, 2014
Nitrogen plays a vital role in the growth, development and nutritional quality of Chinese chive, which is an important leafy vegetable. The effects of improved Hoagland nutrient solutions on the nutritional quality and physiological characteristics of ...
Y.D. Sun, W.R. Luo, H.C. Liu
doaj   +1 more source

Unlocking the potential of cell‐permeable nanobodies for intracellular applications

open access: yesFEBS Letters, EarlyView.
This review outlines current strategies for delivery of nanobodies into cells and highlights recent examples of functional applications. By addressing and manipulating intracellular targets, these molecules hold great potential for treating cancer, respiratory diseases and neurological disorders, making this a vibrant area of research in the biomedical
Mariona Gené‐Verdés   +2 more
wiley   +1 more source

Physiological Responses of Callus from Gerbera jamesonii Bolus ex. Hook f. to Gamma Irradiation

open access: yesBrazilian Archives of Biology and Technology, 2012
In the present study, in vitro mutagenesis techniques were applied to investigate the effects of gamma irradiation at 0, 10, 20, 30, 40, 50 and 60 Gy on physiological changes in callus of Gerbera jamesonii Bolus ex. Hook f.
Nor Azlina Hasbullah   +3 more
doaj   +1 more source

Comparing the paths of Hedgehog release in artificial and natural settings

open access: yesFEBS Letters, EarlyView.
Hh morphogens require solubilization factors to overcome their tight association with the plasma membrane and signal to distant cells, but the precise functions of these factors are controversial. Here, we use published in vivo observations as the most relevant evidence to test their suggested functions and discuss contradictions and gaps between in ...
Janna Puschmann, Kay Grobe
wiley   +1 more source

Properties of soluble protein powders from Alaska pollock (Theragra chalcogramma)

open access: yes, 2006
Soluble protein powders were produced from pollock by-products and evaluated for their functional, nutritional and rheological properties. Soluble protein powders were made from pollock viscera (PVSP), viscera without liver (PVWLSP), heads (PHSP), frames
Sathivel, Subramaniam, Bechtel, Peter J.
core   +1 more source

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