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Reprogramming mammalian somatic cells
Theriogenology, 2012Somatic cell nuclear transfer (SCNT), the technique commonly known as cloning, permits transformation of a somatic cell into an undifferentiated zygote with the potential to develop into a newborn animal (i.e., a clone). In somatic cells, chromatin is programmed to repress most genes and express some, depending on the tissue.
N, Rodriguez-Osorio +4 more
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Somatic Cell Cloning without Micromanipulators
Cloning, 2001Until now, micromanipulators have been regarded as indispensable for somatic cell nuclear transfer. This paper describes an improved zona-free nuclear transfer procedure with manual bisection of oocytes, selection of cytoplasts by Hoechst staining, and two-step fusion of somatic cells from primary granulosa cell cultures with two cytoplasts. Blastocyst
Vajta, G. +4 more
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Reprogramming of Somatic Cells
2012Reprogramming of adult somatic cells into pluripotent stem cells may provide an attractive source of stem cells for regenerative medicine. It has emerged as an invaluable method for generating patient-specific stem cells of any cell lineage without the use of embryonic stem cells.
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The Bovine Practitioner, 2019
Somatic cell counts are only one of several diagnostic aids which may be used to detect nonclinical mastitis. Whenever possible, they should be used with other diagnostic tests such as milk cultures or ELISA type tests to increase the confidence of the decisions.
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Somatic cell counts are only one of several diagnostic aids which may be used to detect nonclinical mastitis. Whenever possible, they should be used with other diagnostic tests such as milk cultures or ELISA type tests to increase the confidence of the decisions.
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1982
Pathway Mutants in Mammalian Cells and their Contribution to the Analysis of Drug Resistance.- Isolation and Analysis of Chinese Hamster Cells Carrying Forward and Reverse Mutations in the Hypoxanthine-Guanine Phosphoribosyltransferase Locus.- Towards Mapping Genes to Chromosomes Using Two Dimensional Gel Electrophoresis of Proteins and Somatic Cell ...
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Pathway Mutants in Mammalian Cells and their Contribution to the Analysis of Drug Resistance.- Isolation and Analysis of Chinese Hamster Cells Carrying Forward and Reverse Mutations in the Hypoxanthine-Guanine Phosphoribosyltransferase Locus.- Towards Mapping Genes to Chromosomes Using Two Dimensional Gel Electrophoresis of Proteins and Somatic Cell ...
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2014
A number of somatic stem cells have been used in inner ear research. In this chapter, two stem cells, mesenchymal and hematopoietic stem cells, are the focus, because these stem cells have already utilized in clinical settings. Of mesenchymal stem cells, the potential of bone marrow- and adipose tissue-derived stem cells for the treatment of the inner ...
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A number of somatic stem cells have been used in inner ear research. In this chapter, two stem cells, mesenchymal and hematopoietic stem cells, are the focus, because these stem cells have already utilized in clinical settings. Of mesenchymal stem cells, the potential of bone marrow- and adipose tissue-derived stem cells for the treatment of the inner ...
openaire +1 more source
1992
Somatic hybridization is a technique which allows the manipulation of cellular genomes by protoplast fusion. Its major contribution to plant breeding is in overcoming common crossing barriers among plant species and in organelle genetics and breeding. Several reviews on different aspects of somatic hybridization were published recently: Pelletier and ...
K. C. Sink, R. K. Jain, J. B. Chowdhury
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Somatic hybridization is a technique which allows the manipulation of cellular genomes by protoplast fusion. Its major contribution to plant breeding is in overcoming common crossing barriers among plant species and in organelle genetics and breeding. Several reviews on different aspects of somatic hybridization were published recently: Pelletier and ...
K. C. Sink, R. K. Jain, J. B. Chowdhury
openaire +1 more source