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Somatic Cell Nuclear Transfer in Pigs
2023Somatic cell nuclear transfer (SCNT) has been successfully applied to clone animals of several species. Pigs are one of the main livestock species for food production and are also important for biomedical research due to their physiopathological similarities with humans.
Werner G, Glanzner +2 more
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Somatic Cell Nuclear Transfer in Rabbits
2023Somatic cell nuclear transfer (SCNT) is a technology that enables differentiated somatic cells to acquire a totipotent state, thus making it of great value in developmental biology, biomedical research, and agricultural applications. Rabbit cloning associated with transgenesis has the potential to improve the applicability of this species for disease ...
Pengxiang, Qu, Wenbin, Cao, Enqi, Liu
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Robotic Batch Somatic Cell Nuclear Transfer Based on Microfluidic Groove
IEEE Transactions on Automation Science and Engineering, 2020Somatic cell nuclear transfer (SCNT), which is an important procedure in cloning, has been conducted manually for decades. The operating efficiency drops sharply in batch SCNT because of the long-time observation under microscopy and the time-wasting ...
Yaowei Liu +4 more
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Reproduction in domestic animals = Zuchthygiene, 2020
Taking into account the latest Red List of the International Union for Conservation of Nature (IUCN) in which 25% of all mammals are threatened with extinction, Somatic Cell Nuclear Transfer (SCNT) could be a beneficial tool and holds a lot of potential ...
P. Mrowiec +2 more
semanticscholar +1 more source
Taking into account the latest Red List of the International Union for Conservation of Nature (IUCN) in which 25% of all mammals are threatened with extinction, Somatic Cell Nuclear Transfer (SCNT) could be a beneficial tool and holds a lot of potential ...
P. Mrowiec +2 more
semanticscholar +1 more source
Cloning by somatic cell nuclear transfer
BioEssays, 1998The birth of the first cloned mammals, produced by the introduction of somatic cell nuclei into enucleated oocytes, was an impressive and surprising development. Although the ethical debate has been intense, the important scientific questions raised by this work have been inadequately discussed and are still unresolved.
J, Fulka, N L, First, P, Loi, R M, Moor
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Bovine Somatic Cell Nuclear Transfer
2010Somatic cell nuclear transfer (SCNT) is a technique by which the nucleus of a differentiated cell is introduced into an oocyte from which its genetic material has been removed by a process called enucleation. In mammals, the reconstructed embryo is artificially induced to initiate embryonic development (activation).
Pablo J, Ross, Jose B, Cibelli
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Somatic cell nuclear transfer in zebrafish
Nature Methods, 2009We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.
Kannika, Siripattarapravat +4 more
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Somatic Cell Nuclear Transfer in Horses
Reproduction in Domestic Animals, 2008ContentsThe cloning of equids was achieved in 2003, several years after the birth of Dolly the sheep and also after the cloning of numerous other laboratory and farm animal species. The delay was because of the limited development in the horse of more classical‐assisted reproductive techniques required for successful cloning, such as oocyte maturation ...
Cesare, Galli +4 more
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Reproduction in domestic animals = Zuchthygiene, 2019
As a natural plant-derived antitoxin, resveratrol possesses several pharmacological activities. This study aimed to evaluate the effects of resveratrol addition on nuclear maturation, oocyte quality during in vitro maturation (IVM) of porcine oocytes ...
Xuefang Wang +6 more
semanticscholar +1 more source
As a natural plant-derived antitoxin, resveratrol possesses several pharmacological activities. This study aimed to evaluate the effects of resveratrol addition on nuclear maturation, oocyte quality during in vitro maturation (IVM) of porcine oocytes ...
Xuefang Wang +6 more
semanticscholar +1 more source
Epigenetic Reprogramming and Somatic Cell Nuclear Transfer
2023Epigenetics is an area of genetics that studies the heritable modifications in gene expression and phenotype that are not controlled by the primary sequence of DNA. The main epigenetic mechanisms are DNA methylation, post-translational covalent modifications in histone tails, and non-coding RNAs. During mammalian development, there are two global waves
Luna N, Vargas +2 more
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