Results 251 to 260 of about 42,166 (286)

Somatic Cell Nuclear Transfer

2019
In biological system, the term “cloning” has different meanings. Animal cloning, the somatic cell nuclear transfer (SCNT) cloning, became a buzzword when Dolly, the first mammalian species was born from the nuclear transfer of fibroblasts taken from adult sheep.
Birbal Singh, Gorakh Mal, Sanjeev K. Gautam, Manishi Mukesh   +3 more
openaire   +1 more source

Animal Cloning by Somatic Cell Nuclear Transfer

2009
Animal cloning is becoming increasingly useful for its applications in biological inquiry and for its potential use in pharmaceutical, medical, and agricultural fields. Due to the complexity of the numerous steps required in reconstructing oocytes by nuclear transfer, detailed protocols are required to minimize the developmental damages inflicted ...
Lawrence C, Smith, Jae-Gyu, Yoo
openaire   +2 more sources

Procedures for Somatic Cell Nuclear Transfer in the Rat

2009
Somatic cell nuclear transfer (SCNT) is a powerful tool for the investigation of the mechanisms of nuclear remodeling. In addition, SCNT may offer the possibility of introducing targeted mutations by homologous recombination in species for which ES cell technology is not available.
Jean, Cozzi, Eryao, Wang, Christelle, Jacquet, Alexandre, Fraichard, Yacine, Cherifi, Qi, Zhou   +5 more
openaire   +2 more sources

Bovid Interspecies Somatic Cell Nuclear Transfer with Ooplasm Transfer

2023
Interspecies somatic cell nuclear transfer (iSCNT) contributes to the preservation of endangered species, albeit nuclear-mitochondrial incompatibilities constrain its application. iSCNT, coupled with ooplasm transfer (iSCNT-OT), has the potential to overcome the challenges associated with species- and genus-specific differences in nuclear-mitochondrial
L Antonio, González-Grajales, Gabriela F, Mastromonaco   +1 more
openaire   +2 more sources

Method for Somatic Cell Nuclear Transfer in Zebrafish

2011
Somatic cell nuclear transfer (SCNT) has been a well-known technique for decades and widely applied to generate identical animals, including ones with genetic alterations. The system has been demonstrated successfully in zebrafish. The elaborated requirements of SCNT, however, limit reproducibility of the established model to a few groups in zebrafish ...
Kannika, Siripattarapravat, Jose B, Cibelli   +1 more
openaire   +2 more sources

Progress in Human Somatic-Cell Nuclear Transfer

New England Journal of Medicine, 2005
The efficiency of deriving blastocysts through the nuclear transfer of embryonic stem cells has been improved by a factor of more than 14.
openaire   +2 more sources

Method for somatic cell nuclear transfer in zebrafish

2016
This chapter presents a detailed methodology for somatic cell nuclear transfer-cloning of zebrafish. We aim to place the reader in a virtual lab experience to assist acquisition of the technical skills required for reproducing the published protocol.
K, Siripattarapravat, S, Prukudom, J, Cibelli   +2 more
openaire   +2 more sources

Improvement of canine somatic cell nuclear transfer procedure

Theriogenology, 2008
The purpose of the present study on canine somatic cell nuclear transfer (SCNT) was to evaluate the effects of fusion strength, type of activation, culture media and site of transfer on developmental potential of SCNT embryos. We also examined the potential of enucleated bovine oocytes to serve as cytoplast recipients of canine somatic cells.
G, Jang, H J, Oh, M K, Kim, Y H, Fibrianto, M S, Hossein, H J, Kim, J J, Kim, S G, Hong, J E, Park, S K, Kang, B C, Lee   +10 more
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Pig Cloning Using Somatic Cell Nuclear Transfer

2020
Porcine cloning technology can be used to produce progenies genetically identical to the donor cells from high-quality breeding pigs. In addition, genetically modified pigs have been produced by somatic cell nuclear transfer using genetically modified porcine fetal fibroblasts.
Hongsheng, Ouyang, Jianyong, Han, Yongye, Huang   +2 more
openaire   +2 more sources

Birth of Beagle dogs by somatic cell nuclear transfer

Animal Reproduction Science, 2009
The present study was undertaken to evaluate two enucleation methods for somatic cell nuclear transfer (SCNT), and to standardize the optimum number of embryos for transfer to each recipient for canines. Oocytes retrieved from outbreed dogs were reconstructed with adult somatic cells from a male Beagle dog. A total of 134 or 267 oocytes were enucleated
Mohammad Shamim, Hossein, Yeon Woo, Jeong, Sun Woo, Park, Joung Joo, Kim, Eugine, Lee, Kyeong Hee, Ko, Park, Hyuk, Song Seung, Hoon, Yeun Wook, Kim, Sang Hwan, Hyun, Taeyoung, Shin, Woo Suk, Hwang   +11 more
openaire   +2 more sources