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Somatic Cell Nuclear Transfer in the Mouse
2008Somatic cell nuclear transfer (SCNT) has become a unique and powerful tool for epigenetic reprogramming research and gene manipulation in animals since "Dolly," the first animal cloned from an adult cell was reported in 1997. Although the success rates of somatic cloning have been inefficient and the mechanism of reprogramming is still largely unknown,
Satoshi, Kishigami, Teruhiko, Wakayama
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Epigenetic Reprogramming and Somatic Cell Nuclear Transfer
2023Epigenetics is an area of genetics that studies the heritable modifications in gene expression and phenotype that are not controlled by the primary sequence of DNA. The main epigenetic mechanisms are DNA methylation, post-translational covalent modifications in histone tails, and non-coding RNAs. During mammalian development, there are two global waves
Luna N, Vargas +2 more
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Bovine Somatic Cell Nuclear Transfer
2010Somatic cell nuclear transfer (SCNT) is a technique by which the nucleus of a differentiated cell is introduced into an oocyte from which its genetic material has been removed by a process called enucleation. In mammals, the reconstructed embryo is artificially induced to initiate embryonic development (activation).
Pablo J, Ross, Jose B, Cibelli
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Cloning of Monkeys by Somatic Cell Nuclear Transfer
2020Somatic cell nuclear transfer (SCNT) is a promising method to establish genetically modified monkeys with identical genetic background as models in biomedical research. We have recently cloned monkeys by optimization of the SCNT protocols and inclusion of the epigenetic modulator.
Shiyu, Sun +3 more
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2019
In biological system, the term “cloning” has different meanings. Animal cloning, the somatic cell nuclear transfer (SCNT) cloning, became a buzzword when Dolly, the first mammalian species was born from the nuclear transfer of fibroblasts taken from adult sheep.
Birbal Singh +3 more
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In biological system, the term “cloning” has different meanings. Animal cloning, the somatic cell nuclear transfer (SCNT) cloning, became a buzzword when Dolly, the first mammalian species was born from the nuclear transfer of fibroblasts taken from adult sheep.
Birbal Singh +3 more
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Animal Cloning by Somatic Cell Nuclear Transfer
2009Animal cloning is becoming increasingly useful for its applications in biological inquiry and for its potential use in pharmaceutical, medical, and agricultural fields. Due to the complexity of the numerous steps required in reconstructing oocytes by nuclear transfer, detailed protocols are required to minimize the developmental damages inflicted ...
Lawrence C, Smith, Jae-Gyu, Yoo
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Procedures for Somatic Cell Nuclear Transfer in the Rat
2009Somatic cell nuclear transfer (SCNT) is a powerful tool for the investigation of the mechanisms of nuclear remodeling. In addition, SCNT may offer the possibility of introducing targeted mutations by homologous recombination in species for which ES cell technology is not available.
Jean, Cozzi +5 more
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Method for Somatic Cell Nuclear Transfer in Zebrafish
2011Somatic cell nuclear transfer (SCNT) has been a well-known technique for decades and widely applied to generate identical animals, including ones with genetic alterations. The system has been demonstrated successfully in zebrafish. The elaborated requirements of SCNT, however, limit reproducibility of the established model to a few groups in zebrafish ...
Kannika, Siripattarapravat +1 more
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Method for somatic cell nuclear transfer in zebrafish
2016This chapter presents a detailed methodology for somatic cell nuclear transfer-cloning of zebrafish. We aim to place the reader in a virtual lab experience to assist acquisition of the technical skills required for reproducing the published protocol.
K, Siripattarapravat +2 more
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Bovid Interspecies Somatic Cell Nuclear Transfer with Ooplasm Transfer
2023Interspecies somatic cell nuclear transfer (iSCNT) contributes to the preservation of endangered species, albeit nuclear-mitochondrial incompatibilities constrain its application. iSCNT, coupled with ooplasm transfer (iSCNT-OT), has the potential to overcome the challenges associated with species- and genus-specific differences in nuclear-mitochondrial
L Antonio, González-Grajales +1 more
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