Results 241 to 250 of about 42,166 (286)

Somatic Cell Nuclear Transfer in Rabbits

2023
Somatic cell nuclear transfer (SCNT) is a technology that enables differentiated somatic cells to acquire a totipotent state, thus making it of great value in developmental biology, biomedical research, and agricultural applications. Rabbit cloning associated with transgenesis has the potential to improve the applicability of this species for disease ...
Pengxiang, Qu, Wenbin, Cao, Enqi, Liu
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Somatic Cell Nuclear Transfer in Pigs

2023
Somatic cell nuclear transfer (SCNT) has been successfully applied to clone animals of several species. Pigs are one of the main livestock species for food production and are also important for biomedical research due to their physiopathological similarities with humans.
Werner G, Glanzner, Vitor B, Rissi, Vilceu, Bordignon   +2 more
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Cloning by somatic cell nuclear transfer

BioEssays, 1998
The birth of the first cloned mammals, produced by the introduction of somatic cell nuclei into enucleated oocytes, was an impressive and surprising development. Although the ethical debate has been intense, the important scientific questions raised by this work have been inadequately discussed and are still unresolved.
J, Fulka, N L, First, P, Loi, R M, Moor
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Somatic cell nuclear transfer in zebrafish

Nature Methods, 2009
We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.
Kannika, Siripattarapravat, Boonya, Pinmee, Patrick J, Venta, Chia-Cheng, Chang, Jose B, Cibelli   +4 more
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Somatic Cell Nuclear Transfer in Horses

Reproduction in Domestic Animals, 2008
ContentsThe cloning of equids was achieved in 2003, several years after the birth of Dolly the sheep and also after the cloning of numerous other laboratory and farm animal species. The delay was because of the limited development in the horse of more classical‐assisted reproductive techniques required for successful cloning, such as oocyte maturation ...
Cesare, Galli, Irina, Lagutina, Roberto, Duchi, Silvia, Colleoni, Giovanna, Lazzari   +4 more
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Cattle Cloning by Somatic Cell Nuclear Transfer

2023
Cloning by somatic cell Nuclear Transfer (SCNT) is a powerful technology capable of reprograming terminally differentiated cells to totipotency for generating whole animals or pluripotent stem cells for use in cell therapy, drug screening, and other biotechnological applications. However, the broad usage of SCNT remains limited due to its high cost and
Juliano Rodrigues, Sangalli, Rafael Vilar, Sampaio, Tiago Henrique Camara, De Bem, Lawrence Charles, Smith, Flávio Vieira, Meirelles   +4 more
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Bovine Somatic Cell Nuclear Transfer

2010
Somatic cell nuclear transfer (SCNT) is a technique by which the nucleus of a differentiated cell is introduced into an oocyte from which its genetic material has been removed by a process called enucleation. In mammals, the reconstructed embryo is artificially induced to initiate embryonic development (activation).
Pablo J, Ross, Jose B, Cibelli
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Epigenetic Reprogramming and Somatic Cell Nuclear Transfer

2023
Epigenetics is an area of genetics that studies the heritable modifications in gene expression and phenotype that are not controlled by the primary sequence of DNA. The main epigenetic mechanisms are DNA methylation, post-translational covalent modifications in histone tails, and non-coding RNAs. During mammalian development, there are two global waves
Luna N, Vargas, Márcia M, Silveira, Maurício M, Franco   +2 more
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Cloning of Monkeys by Somatic Cell Nuclear Transfer

2020
Somatic cell nuclear transfer (SCNT) is a promising method to establish genetically modified monkeys with identical genetic background as models in biomedical research. We have recently cloned monkeys by optimization of the SCNT protocols and inclusion of the epigenetic modulator.
Shiyu, Sun, Zhaodi, Liao, Zhen, Liu, Qiang, Sun   +3 more
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Somatic Cell Nuclear Transfer in the Mouse

2008
Somatic cell nuclear transfer (SCNT) has become a unique and powerful tool for epigenetic reprogramming research and gene manipulation in animals since "Dolly," the first animal cloned from an adult cell was reported in 1997. Although the success rates of somatic cloning have been inefficient and the mechanism of reprogramming is still largely unknown,
Satoshi, Kishigami, Teruhiko, Wakayama
openaire   +2 more sources