Results 51 to 60 of about 111,686 (306)

Prospects for transgenesis in the chick [PDF]

open access: yes, 2004
Research to develop a useful method for genetic modification of the chick has been on-going since the first demonstrations in the mouse in the 1980s that genetic modification is an invaluable tool for the study of gene function. Manipulation of the chick
Bosselman   +52 more
core   +1 more source

Plant Recovery from Sweetpotato Somatic Embryos [PDF]

open access: yesHortScience, 1990
Plant formation from somatic embryos in response to BAP, NAA, and sucrose was studied in sweetpotato [Ipomoea batatas (L.) Lam.]. A maximum of 15% embryos at the torpedo stage of development formed plants of agar-solidified basal medium containing 3% sucrose and no growth regulators. The percentage of embryos forming shoots was increased to 53% by 4 μm
Raymond P. Chée   +2 more
openaire   +1 more source

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

open access: yesMolecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel   +12 more
wiley   +1 more source

Influence of maltose and sucrose on somatic embryos maturation of Glycine max cv. 'Incasoy-27'

open access: yesBiotecnología Vegetal, 2019
The somatic embryogenesis in soybean crop (Glycine max)has as limitation the low percentage of conversion to plant that is obtained, attributed to the presence of somatic embryos with abnormal morphologies during the maturation phase.
Jorge Pérez Pérez   +5 more
doaj  

Current applications of coffee (Coffea arabica) somatic embryogenesis for industrial propagation of elite heterozygous materials in Central America and Mexico [PDF]

open access: yes, 2010
Of ail the possible micropropagation techniques, it is widely accepted that vegetative propagation by somatic embryogenesis is by far the most promising for rapid, large-scale dissemination of elite individuals.
Alpizar, Edgardo   +11 more
core  

GENE EXPRESSION IN PECAN SOMATIC EMBRYOS [PDF]

open access: yesHortScience, 1991
Repetitive somatic embryogenic lines of pecan (Carya illinoensis) were obtained and subcultured on basal WPM, following a one week induction of zygotic embryo tissue on modified WPM with 6 mg/L NAA. Gene expression of somatic embryos has been studied and compared with that occurring in zygotic embryos.
Amnon Levi   +2 more
openaire   +1 more source

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

open access: yesFEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon   +6 more
wiley   +1 more source

Influence of the season of the year and the type of flask in the somatic embryogenesis in papaya var. Maradol rojo

open access: yesBiotecnología Vegetal, 2009
Somatic embryogenesis in papaya has been subjected to many researches. However, the effect of ex vitro and in vitro environment on this process has been little studied.
Laisyn Posada-Pérez   +4 more
doaj  

Efficient plant regeneration from embryogenic cell suspension cultures of Euonymus alatus

open access: yesScientific Reports, 2021
To establish an efficient plant regeneration system from cell suspension cultures of Euonymus alatus, embryogenic callus formation from immature embryos was investigated. The highest frequency of embryogenic callus formation reached 50% when the immature
Hyun-A Woo   +9 more
doaj   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

open access: yesFEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li   +8 more
wiley   +1 more source

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