Results 61 to 70 of about 111,686 (306)
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
FEBS Open Bio, EarlyView.Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Biotecnología Vegetal, 2011 Soybean is genotype-dependent as it has been shown. This has lead to work on new protocols for plant regeneration. This research aimed to achieve growth and germination of somatic embryos in the Cuban varieties Incasoy-1 and Incasoy-27.
Idalmis Bermúdez-Caraballoso +6 more
doaj
Live pigs produced from genome edited zygotes [PDF]
, 2013 Transcription activator-like effector nuclease (TALEN) and zinc finger nuclease (ZFN) genome editing technology enables site directed engineering of the genome.
AJ Clark +13 more
core +1 more source
Derivation and characterization of retinal pigment epithelium from urine‐derived iPSCs
FEBS Open Bio, EarlyView.Age‐related macular degeneration causes vision loss via RPE dysfunction and loss. Traditional iPSC therapies rely on invasive biopsies, limiting scalability. Here, we utilize urine‐derived stem cells as an accessible source to generate u‐iPSCs, successfully differentiated into pigmented RPE. This “Urine‐to‐Retina” platform provides a promising path for
Daniella Beiner +7 more
wiley +1 more source
Biotecnología Vegetal, 2013 Low germination of somatic embryos in S. mahagoni is a problem that needs solutions. Its cause is related to the low level of embryo maturation. The objective of this research was to determine the effect of different concentrations of sorbitol and 6-BAP ...
Raúl Barbón +4 more
doaj
The Scientific World Journal, 2013 Embryogenic callus was obtained from mature seed explants on medium supplemented with 2,4-dichlorophenoxyacetic acid. Primary somatic embryos (SEs) can only develop into abnormal plants.
Jingli Yang, Songquan Wu, Chenghao Li
doaj +1 more source
Bulletin of the National Research Centre, 2019 Background Somatic embryogenesis is one of the most popular in vitro regeneration methods for mass micropropagation. In the present study, somatic embryogenesis via zygotic embryos was studied in Asparagus racemosus Willd.
Juhi Chaudhary, Prem Kumar Dantu
doaj +1 more source
Kanamycin Sensitivity of Mango Somatic Embryos [PDF]
HortScience, 1990 Mango (Mangifera indica L.) somatic embryos representing various developmental stages were subjected to various concentrations of kanamycin in the culture medium. The level of kanamycin necessary for growth inhibition was dependent on the size and stage of the somatic embryos at the time of treatment and the kind of exposure.
Helena Mathews, Richard E. Litz
openaire +1 more source
Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance
Aging and Cancer, EarlyView.NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang +3 more
wiley +1 more source
Comparative Analysis of Nuclear Transfer Embryo Derived Mouse Embryonic Stem Cells. Part I: Cellular characterization [PDF]
, 2012 Embryonic stem cells derived from nuclear transfer embryos (ntESCs) are particularly valuable for regenerative medicine, as they are a patient-specific and histocompatible cell source for the treatment of varying diseases.
Csonka, Erika +6 more
core +1 more source