Results 61 to 70 of about 483,171 (293)

Using cell‐free RNA to identify B‐ and T‐cell clonality for diagnosis and monitoring of B‐ and T‐cell neoplasms

FEBS Open Bio, EarlyView.
Using peripheral blood for determining B‐cell or T‐cell clonality is more reliable when we use cell‐free RNA (cfRNA) because cells release blood significantly more RNA than DNA. Next‐generation sequencing (NGS) of cfRNA allows us to evaluate fragment cfRNA and evaluate clonality reliably without the need for prior determination of the specific dominant
Adam Albitar, Sally Agersborg, Ahmed Charifa, Pooja Phull, Noa Biran, David Vesole, Harsh Parmar, Andrew Pecora, Andrew Ip, Andre Goy, David Siegel, Maher Albitar   +11 more
wiley   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

FEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav, Shweta Shweta, Manisha Malhotra, Vikash Maurya, Akhilesh Kumar   +4 more
wiley   +1 more source

Somatic hybridization for citrus rootstock breeding: an effective tool to solve some important issues of the Mediterranean citrus industry

, 2011
The prevalence of sour orange rootstock in the southern and eastern part of the Mediterranean Basin is presently threatened by the spread of Citrus Tristeza Virus (CTV) and its main vector Toxoptera citricida, combined with abiotic constraints such as ...
Zina Belfalah, Benyahia, Hamid, Giovanni Pensabene-Bellavia, Pensabene-Bellavia, Giovanni, Najat Handaji, Yann Froelicher, Belfalah, Zina, Beniken Lhou, Dambier, Dominique, Yesiloglu, Turgut, Turgut Yesiloglu, Printz, Bruno, Ollitrault, Patrick, Bruno Printz, Patrick Ollitrault, Lhou, Beniken, Froelicher, Yann, Beniken, Lhou, Yildiz Aka Kaçar, Navarro, Luis, Dominique Dambier, Raphael Morillon, Handaji, Najat, Luis Navarro, Hamid Benyahia, Kacar, Yildiz Aka, Aka Kaçar, Yildiz, Dambier D., Kaçar Y.A., Benyahia H., Pensabene-Bellavia G., Morillon, Raphael, Lhou B., Belfalah Z., Froelicher Y.   +34 more
core   +2 more sources

Molecular Characterization and Stress Tolerance Evaluation of New Allotetraploid Somatic Hybrids Between Carrizo Citrange and Citrus macrophylla W. rootstocks

Frontiers in Plant Science, 2018
Polyploidy is one of the main forces that drives the evolution of plants and provides great advantages for breeding. Somatic hybridization by protoplast fusion is used in citrus breeding programs.
Marta Ruiz, Giovanni Pensabene-Bellavia, Ana Quiñones, Andrés García-Lor, Raphaël Morillon, Patrick Ollitrault, Eduardo Primo-Millo, Luis Navarro, Pablo Aleza   +8 more
doaj   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Advancing Citrus Breeding: Next- Genereation Tools for Resistance, Flavor and Health

Horticulturae
New plant breeding techniques are revolutionizing citrus improvement by accelerating trait enhancement and genetic gains. In recent years, technological advances have enabled more precise and accelerated breeding.
David Ezra, Nir Carmi
doaj   +1 more source

Hydrogel platforms for 3D cardiac tissue engineering and fibrotic disease modelling

FEBS Open Bio, EarlyView.
Hydrogel‐based cardiac tissue models are advancing our understanding of heart health and disease. By incorporating electrical conductivity, aligned structures, and tunable mechanical properties, these platforms better replicate the complex environment of native heart tissue.
Harrison Porritt, Annika Winbo, Jenny Malmström   +2 more
wiley   +1 more source

Chronobiology of Cancer: How Aging Fuels Oncogenesis at the Molecular Level

Aging and Cancer, EarlyView.
This graphical abstract illustrates the key biological pathways linking aging with cancer development and progression. In the upper left, cumulative exposure to ultraviolet radiation, toxins, and reactive oxygen species (ROS) causes DNA damage and genomic instability, whereas age‐related decline in repair mechanisms, such as ATM/ATR, BER, and NER ...
Anu Singh, Aroonima Misra, Sufian Zaheer
wiley   +1 more source

Somatic hybridization in ornamental plants

, 2014
The basic methods for somatic hybridization were elaborated more then 30 years ago. Somatic hybridization is a method with a high potential to create new variability and to use wild genetic resources.
Klocke, Evelyn
core  

Somatic hybridization of amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) by electrofusion

, 1987
Intraspecific somatic hybridization between amino acid analogue-resistant cell lines of potato (Solanum tuberosum L.) has been carried out following electrofusion of protoplasts. In initial analytical electrofusion experiments (1 mm electrode separation)
Feenstra, W. J., JONES, MGK, LOONEN, AEHM, Jacobsen, E., Tempelaar, M.J., Tempelaar, M. J., Loonen, A. E. H. M., Feenstra, W.J., DEVRIES, SE, WIJBRANDI, J, Jones, M.G.K., Loonen, A. E. H.M., Jones, M. G. K., Wijbrandi, J., De Vries, S.E., Loonen, A.E.H.M., De Vries, S. E.   +16 more
core   +1 more source