Results 111 to 120 of about 20,554 (213)

Affinity Maturation Enhances Antibody Specificity but Compromises Conformational Stability

Cell Reports, 2019
Summary: Monoclonal antibodies (mAbs) have recently emerged as one of the most promising classes of biotherapeutics. A potential advantage of B cell-derived mAbs as therapeutic agents is that they have been subjected to natural filtering mechanisms ...
Laila Shehata, Daniel P. Maurer, Anna Z. Wec, Asparouh Lilov, Elizabeth Champney, Tingwan Sun, Kimberly Archambault, Irina Burnina, Heather Lynaugh, Xiaoyong Zhi, Yingda Xu, Laura M. Walker   +11 more
doaj   +1 more source

Ectopic Lymphoid Structures Support Ongoing Production of Class-Switched Autoantibodies in Rheumatoid Synovium

, 2009
BackgroundFollicular structures resembling germinal centres (GCs) that are characterized by follicular dendritic cell (FDC) networks have long been recognized in chronically inflamed tissues in autoimmune diseases, including the synovium of rheumatoid ...
Kelly, Stephen, Kelly, S, Blades, Mark C., Kirkham, Bruce, Spencer, Jo, Frances Humby, Bruce Kirkham, Pitzalis, Costantino, Michele Bombardieri, Kelly Stephen, Mark C Blades, Humby, F, Bombardieri M., Blades, MC, Manzo Antonio, Humby, Frances, Stephen Kelly, Bombardieri, M, Pitzalis C., Bombardieri, Michele, Spencer, J, Humby F., Blades M.C., Bombardieri Michele, Humby Frances, Blades Mark C, Kirkham, B, Kirkham Bruce, Manzo, A, Spencer Jo, Spencer J., MANZO, ANTONIO, Pitzalis Costantino, Antonio Manzo, Pitzalis, C, Kirkham B., Jo Spencer, Costantino Pitzalis, Kelly S.   +38 more
core   +1 more source

FACS Staining for Follicular Helper T Cells

Bio-Protocol, 2012
Germinal center is the primary site for B cells to undergo somatic hypermutation and class switching. A recently discovered subset of T cells known as T follicular helper (TFH) cells are essential for germinal center reaction and thus have been the ...
Zheng Liu
doaj   +1 more source

Somatic Hypermutation of Immunoglobulin Genes Is Linked to Transcription Initiation

, 1996
To identify DNA sequences that target the somatic hypermutation process, the immunoglobulin gene promoter located upstream of the variable (V) region was duplicated upstream of the constant (C) region of a κ transgene.
Ursula Storb, Peters, Andrew, Andrew Peters, Storb, Ursula   +3 more
core   +1 more source

Impact of DNA ligase IV on nonhomologous end joining pathways during class switch recombination in human cells

, 2005
Class switch recombination (CSR) is a region-specific, transcriptionally regulated, nonhomologous recombinational process that is initiated by activation-induced cytidine deaminase (AID).
Lennart Hammarström, Hammarström, L,, Lahdesmaki, A, Aleksi Lähdesmäki, Wei Zhou, Andrew R. Gennery, Jones, AM, Michael R. Ehrenstein, Pan-Hammarstrom, Q, Hammarstrom, L, Zhou, W, Gennery, AR, Ehrenstein, MR, Anne-Marie Jones, Richard A. Gatti, Gatti, RA, Qiang Pan-Hammarström   +16 more
core   +1 more source

The E Box Motif CAGGTG Enhances Somatic Hypermutation without Enhancing Transcription

, 2003
The frequency of somatic hypermutations of an Ig κ transgene with an artificial test insert, RS, is at least 4-fold higher than that of three related transgenes. The four transgenes differ only in the sequence of a 96 bp insert within the variable region.
Kim, Nayun, Michael, Nancy, Longacre, Angelika, Simonne Longerich, Longerich, Simonne, Ursula Storb, Shen, Hong Ming, Hong Ming Shen, Storb, Ursula, Nayun Kim, Nancy Michael, Angelika Longacre   +11 more
core   +1 more source

Breakpoints in the ataxia telangiectasia gene arise at the RGYW somatic hypermutation motif.

, 2002
The mature sporadic T-cell malignancy, T-cell prolymphocytic leukemia (T-PLL) is remarkable for frequently harbouring somatic mutations of the Ataxia Telangiectasia (A-T) gene, ATM.
Yuille, MR; id_orcid, Catovsky, D, Matutes, E, Condie, A, Bradshaw, PS   +4 more
core   +1 more source

The boundaries of the distribution of somatic hypermutation of rearranged immunoglobulin variable genes

, 2015
Available evidence about the mechanisms and distribution of somatic hypermutation (SHM) of rearranged immunoglobulin (IgV) genes is reviewed with particular emphasis on the 5′ boundary.
Franklin, Andrew, Blanden, Robert, Steele, Edward J   +2 more
core   +1 more source

A mathematical model for germinal centre kinetics and affinity maturation

, 2002
We present a mathematical model which reproduces experimental data on the germinal centre (GC) kinetics of the primed primary immune response and on affinity maturation observed during the reaction.
DAGMAR IBER, PHILIP K. MAINI, Maini, P. K., Iber, D., Maini, Philip, Maini, PK, Iber, D   +6 more
core   +1 more source

APE2 Promotes AID-Dependent Somatic Hypermutation in Primary B Cell Cultures That Is Suppressed by APE1. [PDF]

J Immunol, 2023
Schrader CE, Williams T, Pechhold K, Linehan EK, Tsuchimoto D, Nakabeppu Y.   +5 more
europepmc   +1 more source