Results 1 to 10 of about 223,319 (307)

Automated design of genomic Southern blot probes [PDF]

BMC Genomics, 2010
Background Sothern blotting is a DNA analysis technique that has found widespread application in molecular biology. It has been used for gene discovery and mapping and has diagnostic and forensic applications, including mutation detection in patient ...
Komiyama Noboru H, Fricker David G, Croning Mike DR, Grant Seth GN   +3 more
doaj   +2 more sources

Universal Southern blot protocol with cold or radioactive probes for the validation of alleles obtained by homologous recombination. [PDF]

Methods, 2021
Codner GF, Erbs V, Loeffler J, Chessum L, Caulder A, Jullien N, Wells S, Birling MC, Teboul L.   +8 more
europepmc   +2 more sources

A simplified method to calculate telomere length from Southern blot images of terminal restriction fragment lengths [PDF]

BioTechniques, 2020
Southern blotting of DNA terminal restriction fragment lengths is the gold standard for measuring mean telomere length. Analysis of the final image is a crucial step in this process, however, current techniques are cumbersome and prone to error.
Lisa F Lincz, Fiona E Scorgie, Madhu B Garg, Jayne Gilbert, Jennette A Sakoff   +4 more
doaj   +2 more sources

Detection of Cryptic Fragile X Full Mutation Alleles by Southern Blot in a Female and Her Foetal DNA via Chorionic Villus Sampling, Complicated by Mosaicism for 45,X0/46,XX/47,XXX. [PDF]

Genes (Basel), 2021
Pandelache A, Francis D, Oertel R, Dickson R, Sachdev R, Ling L, Gamage D, Godler DE.   +7 more
europepmc   +3 more sources

Optimized Southern blotting for enhanced and precise detection of transgenes in CHO cells from transposon-based expression systems

BioTechniques
The genetic stability of recombinant CHO cell lines producing therapeutic proteins is critical for ensuring consistent quality in biopharma­ceutical products. Southern blotting remains the gold standard for evaluating transgene integrity and stability in
Hyo-Young Jeong, Caitlyn Devine, Bor-Ruei Lin, Zhenqiu Huang, Guanghua Li, Lin Zhang   +5 more
doaj   +2 more sources

Ode to the humble Southern blot in the era of exomes. [PDF]

Neurol Clin Pract, 2018
Paciorkowski AR.
europepmc   +3 more sources

A dual color Southern blot to visualize two genomes or genic regions simultaneously. [PDF]

J Virol Methods, 2014
Zavala AG, Kulkarni AS, Fortunato EA.
europepmc   +3 more sources

A comprehensive analysis of the importance of translation initiation factors for Haloferax volcanii applying deletion and conditional depletion mutants [PDF]

, 2013
Translation is an important step in gene expression. The initiation of translation is phylogenetically diverse, since currently five different initiation mechanisms are known.
Gäbel, Katrin, Näther, Daniela, Schmitt, Jessica, Schulz, Sebastian, Soppa, Jörg   +4 more
core   +15 more sources

Overexpression of a stilbene synthase gene from Fallopia multiflora causes trans-piceid accumulation in transgenic Arabidopsis

Biologia Plantarum, 2020
Fallopia multiflora, also known as Polygonum multiflorum, is a traditional Chinese medicine with stilbenes as the main characteristic components. We previously isolated the presumed stilbene synthase gene FmSTS1 from F. multiflora and inserted the FmSTS1
S. SHENG, W. XIA, W. RUI, W. ZHAO, T. SHE, J. XIE, S. ZHAO, L. CHEN   +7 more
doaj   +1 more source

Impartial comparative analysis of measurement of leukocyte telomere length/DNA content by Southern blots and qPCR. [PDF]

, 2011
Telomere length/DNA content has been measured in epidemiological/clinical settings with the goal of testing a host of hypotheses related to the biology of human aging, but often the conclusions of these studies have been inconsistent.
Aviv, Abraham, Blackburn, Elizabeth, Cao, Xiaojian, Hunt, Steven C, Kimura, Masayuki, Lin, Jue   +5 more
core   +2 more sources