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Brief guide to western blot assays [PDF]

Molecules and Cells
: Western blot is an essential method that detects specific proteins using antibodies, which is one of the most widely applied techniques for protein detection.
Seokjun G. Ha, Ji-Hoon Park, Mi-Young Kim, Seung-Jae V. Lee   +3 more
doaj   +2 more sources

A Simple, Reproducible Procedure for Chemiluminescent Western Blot Quantification

Bio-Protocol, 2023
Western blotting is a universally used technique to identify specific proteins from a heterogeneous and complex mixture. However, there is no clear and common procedure to quantify the results obtained, resulting in variations due to the different ...
DANIEL CAÑADA GARCÍA, Juan Arévalo
doaj   +2 more sources

Metanalysis of the Diagnostic Performance of Western Blot for the Early Diagnosis of Congenital Toxoplasmosis

International Journal of Medical Students, 2023
BACKGROUND: Congenital toxoplasmosis is an infection caused by the Toxoplasma gondii a protozoa obligate intracellular parasite that appears after a mother´s primary infection occurs during pregnancy.
Sebastián Serna Rivera, María Antonia Restrepo Duque, Jocelyn Alexandra Arredondo Torres, Juan David Fandiño López, Luis Felipe Mosquera Solano, Jorge E. Gómez M   +5 more
doaj   +1 more source

A Ponceau S Staining-Based Dot Blot Assay for Rapid Protein Quantification of Biological Samples

Gels, 2022
Despite the availability of a wide range of commercial kits, protein quantification is often unreliable, especially for tissue-derived samples, leading to uneven loading in subsequent experiments. Here we show that the widely used Bicinchoninic Acid (BCA)
Dario Lucas Helbing, Leopold Böhm, Nova Oraha, Leonie Karoline Stabenow, Yan Cui   +4 more
doaj   +1 more source

Deciphering the human antibody response against Burkholderia pseudomallei during melioidosis using a comprehensive immunoproteome approach

Frontiers in Immunology, 2023
IntroductionThe environmental bacterium Burkholderia pseudomallei causes the often fatal and massively underreported infectious disease melioidosis.
Gabriel E. Wagner, Thomas Franz Paul Stanjek, Dirk Albrecht, Michaela Lipp, Susanna J. Dunachie, Susanna J. Dunachie, Susanna J. Dunachie, Esther Föderl-Höbenreich, Esther Föderl-Höbenreich, Katharina Riedel, Anne Kohler, Ivo Steinmetz, Ivo Steinmetz, Christian Kohler   +13 more
doaj   +1 more source

Aptamer Validation by Western Blot–an overview

Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022
Western blot is the main and basic technique in cellular and molecular biology. The principle of the western blot is the isolation and detection of the target molecule usually from a cellular extract.
Lamees Jamal Talib , Basma Talib Al-Sudani , Mustafa Ghazi Al-Abbassi   +2 more
doaj   +1 more source

Improving rigor and reproducibility in western blot experiments with the blotRig analysis [PDF]

Scientific Reports
Adam R Ferguson, Chou Austin, Ferguson Adam R   +2 more
exaly   +2 more sources

Evaluation of Echinococcosis Pre-Diagnosis Patients Admitted to the National Parasitology Reference Laboratory of Turkey from 2014-2019

Iranian Journal of Parasitology, 2022
Background: Echinococcosis is a common parasite with zoonotic character created by a small cestode, Echinococcus spp., and is an important public health problem in Turkey as well as all over the world. We aimed to investigate antibodies in serum samples
Banuçiçek Yücesan, Cahit Babür, Selçuk Kılıç, Asiye Uğraş Dikmen   +3 more
doaj   +1 more source

Patterns of serologic response to human immunodeficiency virus type 1 (HIV-1) in brazilians with different clinical forms of HIV infection Padrões de resposta sorológica ao vírus da imunodeficiência humana do tipo 1 (HIV-1) em brasilieros com diferentes formas clínicas de infecção

Memorias do Instituto Oswaldo Cruz, 1989
In order to investigate the IgG HIV-1 antibodies rectivity to structural components of the virus, 85 sera from infected Brazilians, comprising the total spectrum of HIV infection, were analysed by Western blot assay.
Jairo Ivo dos Santos, Deise Luci Campos, Bernardo Galvão Castro   +2 more
doaj   +1 more source

A Serological Survey on Swine Brucellosis Using Standard Procedures, Dot Blot, and Western Blot in Finisher Pigs in Central-North Italy

Veterinary Sciences, 2018
In recent years, Brucella suis has been sporadically reported in Italy in domestic and wild swine. Since standard serological tests can determine false positive results, the development of alternative tests with improved sensitivity and specificity is ...
Fabrizio Bertelloni, Mario Forzan, Barbara Turchi, Simona Sagona, Maurizio Mazzei, Antonio Felicioli, Filippo Fratini, Domenico Cerri   +7 more
doaj   +1 more source