In the preparation of transgenic murine ES cells it is important to verify the construct has a single insertion, because an ectopic neomycin phosphortransferase positive selection cassette (NEO) may cause a position effect.
Mancini Cecilia +4 more
doaj +1 more source
Organization of aerobactin, hemolysin, and antibacterial resistance genes in lactose negative Escherichia coli strains of serotype O4 isolated from children with dhiarrea [PDF]
Epidemiologically related, non-lactose-fermenting (NLF) Escherichia coli strains of serotype O4 have been isolated at a high frequency from children with diarrhea in Somalia (M. Nicoletti, F. Superti, C. Conti, A. Calconi, and C. Zagaglia, J.
Calconi, Attilio +5 more
core +1 more source
Distinct, developmental stage-specific activation mechanisms of trypanosome VSG genes [PDF]
The metacyclic form of African trypanosomes is the first to express genes for the Variant Surface Glycoprotein (VSG) and it uses an unusually predictable subset of the VSG gene repertoire.
Barry, J D +3 more
core +1 more source
Modifying Glucose Metabolism Reverses Memory Defects of Alzheimer's Disease Model at Late Stages
Using spatial transcriptomics, we show that ferul enanthate (SL‐ZF‐01) reverses episodic‐like memory deficits in aged, but not young, Alzheimer’s disease (AD) mice. SL restores glucose metabolism and Glucose Transporter 1/3 expression via an ‘Aging‐AD‐Rescue’ pattern, rescuing deficits seen in aged AD mice.
Fang Liu +14 more
wiley +1 more source
Antibody-resistant mutants of Borrelia burgdorferi: in vitro selection and characterization. [PDF]
We used polyclonal antisera and monoclonal antibodies (mAbs) to inhibit the growth of clonal populations of two strains of Borrelia burgdorferi, the Lyme disease agent, and thereby select for antibody-resistant mutants.
Barbour, AG +4 more
core
Localization of the human prealbumin gene to 18p11.1-q12.3 by gene dose effect study of Southern blot hybridization [PDF]
Yoshihiro Jinno +7 more
openalex +1 more source
This study demonstrates that Mn2⁺–tumor DNA complexes encapsulated in dendritic cell (DC)– derived immunogenic extracellular vesicles (EVDC@Mn‐DNA) act as a DC‐specific cGAS– STING activator. EVDC@Mn‐DNA treatment enhances intratumoral DC activation, improves tumor vascular function, promotes CD8⁺ T cell activity, and suppresses pancreatic tumor growth,
Xue Jiang +13 more
wiley +1 more source
A modified reverse transcription polymerase chain reaction (RT-PCR)-based differential display procedure with selected primers (SPR) was developed to increase the bias toward isolating moderate- to low-abundance transcripts that are differentially ...
Ognian C. Ikonomov, Michele H. Jacob
doaj +1 more source
Differential regulation of expression of the multiple ADP/ATP translocase genes in human cells [PDF]
The expression of the genes encoding the three isoforms of the human ADP/ATP translocase (T1, T2, and T3) has been investigated in cultured cell systems under different experimental conditions, using isoform- specific probes.
Attardi, Giuseppe, Lunardi, Joël
core
Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and Southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting Candida albicans [PDF]
C. Pujol +5 more
openalex +1 more source

