Results 21 to 30 of about 223,319 (307)
Rapid identification and differentiation of the vaccine strain Rac H from EHV 1 field isolates using a non-radioactive DNA probe [PDF]
, 1992 A method for rapid differentiation between the EHV 1 live vaccine strain Rac H and field isolates is described. Total DNA was isolated from virus-infected small scale cell cultures.
Eichhorn, Werner +3 more
core +1 more source
Human CLPP reverts the longevity phenotype of a fungal ClpP deletion strain [PDF]
, 2013 Mitochondrial maintenance crucially depends on the quality control of proteins by various chaperones, proteases and repair enzymes. While most of the involved components have been studied in some detail, little is known on the biological role of the ...
Fischer, Fabian +3 more
core +1 more source
Molecular cloning and characterization of nucleoside diphosphate kinase 1 cDNA in tea
Biologia Plantarum, 2012 Nucleoside diphosphate kinase (NDPK) operates in the homeostasis of cellular nucleoside triphosphate (NTP) pools and the cytosolic NDPK1 is the main NDPK isoform in plants, accounting for more than 70 % of total NDPK activity in plant. A full length cDNA
G. R. Prabu +3 more
doaj +1 more source
Biologia Plantarum, 2013 Photosynthetic organisms synthesize the amphipathic antioxidants called tocopherols which are essential components of the human diet. To increase the α-tocopherol (vitamin E) content, Arabidopsis genes encoding homogentisate phytyltransferase (HPT) and ...
M. C. Harish +4 more
doaj +1 more source
Targeted mutagenesis of the Sap47 gene of Drosophila: Flies lacking the synapse associated protein of 47 kDa are viable and fertile [PDF]
, 2004 BACKGROUND: Conserved proteins preferentially expressed in synaptic terminals of the nervous system are likely to play a significant role in brain function.
Becker, Sonja +4 more
core +3 more sources
Characterization of two BHV-4 strains isolated in the Czech Republic
Veterinární Medicína, 2010 This study describes the isolation of bovine herpesvirus 4 (BHV-4) from the respiratory tract of animals suffering from respiratory disease. DNA of new isolates, CH and Ni, was cleaved with BamHI, EcoRI and HindIII in restriction enzyme analysis and the ...
V. Fichtelova, K. Kovarcik
doaj +1 more source
RAPID SOUTHERN BLOT PROTOCOL FOR THE ANALYSIS OF TARGETED MUTAGENESIS IN THE MOUSE IGHG1 LOCUS
Scientific Papers Animal Science and Biotechnologies, 2023 The efficiency of homologous recombination relies on the lenght of the homology arms and the chromatin compactation of the locus. In ES cells the immuoglobulin locu is compacted. Thus, the homologous recombination events occur at a very low frequency.
ALENA A. BENCSIK +3 more
doaj
Biologia Plantarum, 2012 A cDNA encoding γ-tocopherol methyltransferase from Brassica napus (BnTMT) was overexpressed in soybean [Glycine max (L.) Merr.] under the control of seed-specific promoter of Arabidopsis fatty acid elongase 1 (FAE1) or soybean glycinin G1. Two and three
D. F. Chen +3 more
doaj +1 more source
Biologia Plantarum, 2010 MAT (multi-auto-transformation) vector system has been one of the strategies to excise the selection marker gene from transgenic plants. Agrobacterium tumefaciens strain EHA105 harboring an ipt-type MAT vector, pNPI132, was used to produce ...
R. S. Khan, I. Nakamura, M. Mii
doaj +1 more source
Selection of co-transformed Dendrobium Sonia 17 using hygromycin and green fluorescent protein
Biologia Plantarum, 2011 Dendrobium Sonia 17 calluses were used for co-transformation study using particle bombardment. The bombarded transformed callus tissues were selected using half-strength MS medium containing 25 mg dm-3 hygromycin. Expression of green fluorescent protein (
C. S. Tee +3 more
doaj +1 more source