Ribosomal DNA (rDNA) spacer polymorphism in mole rats. [PDF]
Genetic differentiation of ribosomal DNA (rDNA) nontranscribed-spacer (NTS) polymorphism was analyzed in 50 individuals from 13 populations among the four chromosomal species (2n = 52, 2n = 54, 2n = 58, and 2n = 60) of subterranean mole rats of the Spalax ehrenbergi complex in Israel.
H, Suzuki, K, Moriwaki, E, Nevo
openaire +2 more sources
CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation
CAPPs are putative Primase-Polymerases associated with CRISPR-Cas operons. Here, the authors show CAPPs genetic and physical association with Cas1 and Cas2, their capacity to function as DNA-dependent DNA primases and DNA polymerases, and that Cas1-Cas2 ...
Katerina Zabrady +4 more
doaj +1 more source
Are DNA spacers relics of gene amplification events? [PDF]
The genome of a thermophilic fungus, Thermomyces lanuginosus, contains a tandemly arranged cluster of sequences that are approximately equal to 50% homologous with the cytoplasmic 5S RNA and that are selectable by hybridization techniques. Unlike typical pseudogenes, these sequences are not truncated; rather, they bear a limited sequence homology with ...
W M, Wong, J L, Abrahamson, R N, Nazar
openaire +2 more sources
Transcriptional role for the nontranscribed spacer of rat ribosomal DNA. [PDF]
In vitro transcription of the rat rRNA gene led to the identification of a region within a 3.4-kilobase fragment of the nontranscribed spacer (NTS) which significantly increased the transcription of rat ribosomal DNA. Promoter constructs containing this region were transcribed up to 17-fold more efficiently in vitro than templates with only 167 or 286 ...
B G, Cassidy +2 more
openaire +2 more sources
CRISPR-Cas is a bacterial immune system that restricts the acquisition of mobile DNA elements. These systems provide immunity against foreign DNA by encoding CRISPR spacers that help target DNA if it re-enters the cell.
Alejandro R. Walker, Robert C. Shields
doaj +1 more source
Physical Model of the Immune Response of Bacteria Against Bacteriophage Through the Adaptive CRISPR-Cas Immune System [PDF]
Bacteria and archaea have evolved an adaptive, heritable immune system that recognizes and protects against viruses or plasmids. This system, known as the CRISPR-Cas system, allows the host to recognize and incorporate short foreign DNA or RNA sequences,
Barrick, Jeffrey E. +3 more
core +1 more source
A Newly Discovered Bordetella Species Carries a Transcriptionally Active CRISPR-Cas with a Small Cas9 Endonuclease [PDF]
Background Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated genes (cas) are widely distributed among bacteria.
Dudley, Edward G. +7 more
core +2 more sources
Application of rps16 Intron and trnL-trnF Intergenic Spacer Sequences to Identify Rengas Clone Riau
Rengas clone Riau has been identified using morphological characters and molecular technique with a psbA-trnH intergenic spacer, however, this method can only determine its taxonomic status at genus level, namely Gluta sp. This study reports application
Dewi Indriyani Roslim, Herman Herman
doaj +1 more source
An Active Type I-E CRISPR-Cas System Identified in Streptomyces avermitilis. [PDF]
CRISPR-Cas systems, the small RNA-dependent immune systems, are widely distributed in prokaryotes. However, only a small proportion of CRISPR-Cas systems have been identified to be active in bacteria.
Yi Qiu +4 more
doaj +1 more source
Transcription in cloned spacers of Xenopus laevis ribosomal DNA. [PDF]
Rare individuals of Xenopus laevis exhibit frequent initiation of transcription in the spacers of oocyte ribosomal DNA (rDNA). Using electron microscopy we have characterized spacer transcription in such an individual and have confirmed that the sites of transcription initiation correspond to the imperfectly duplicated promoters ("Bam islands") present
G T, Morgan, R H, Reeder, A H, Bakken
openaire +2 more sources

