RAPID-DASH: fast and efficient assembly of guide RNA arrays for multiplexed CRISPR-Cas9 applications. [PDF]
Salaudeen AL, Mateyko N, de Boer CG.
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Bioinformatic Identification of CRISPR-Cas Systems in <i>Leptospira</i> Genus: An Update on Their Distribution Across 77 Species. [PDF]
Peláez Sánchez RG +9 more
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A Modular T7 RNA Polymerase Toolbox Linking Selective miRNA Detection to Signal Amplification and Protein Expression. [PDF]
Vonk-de Roy M, Walther A.
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Research note: High-resolution detection of Salmonella serovar diversity in broilers from Ecuador using CRISPR-SeroSeq. [PDF]
Vinueza-Burgos C +6 more
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Schistosoma Species and Hybrid Genotyping With a Field Deployable Multi-Marker Amplicon Panel. [PDF]
Ajakaye OG, Grigg ME.
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Nontranscribed spacers in Drosophila ribosomal DNA
Chromosoma, 1981Ribosomal DNA nontranscribed spacers in Drosophila virilis DNA have been examined in some detail by restriction site analysis of cloned segments of rDNA, nucleic acid hybridizations involving unfractionated rDNA, and base composition estimates. The overall G + C content of the spacer is 27--28%; this compares with 39% for rDNA as a whole, 40% for main ...
P M, Rae, T, Barnett, V L, Murtif
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Patterns of ribosomal DNA spacer lengths are inherited
Journal of Molecular Biology, 1976Abstract The non-transcribed spacer regions in Xenopus laevis ribosomal DNA vary in length, even within a single nucleolar organizer. The pattern of spacer lengths is sufficiently different from one nucleolar organizer to another to allow the pattern to be used as a genetic marker.
R H, Reeder +3 more
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Ribosomal DNA spacer-length variation inSecale spp. (Poaceae)
Plant Systematics and Evolution, 1990Variation in ribosomal DNA spacer length was analysed in 23 populations of 12Secale spp. by restriction enzyme analysis. Digestion of rDNA with Taq I endonuclease enzyme yields spacer fragments that include the subrepeat array and the non-repetitive region downstream of the array.
P. Reddy, R. Appels, B. R. Baum
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An internal transcribed spacer (ITS2) sequence between the 5.8S and 28S rRNA genes was used to estimate the phyletic relationships among Ixodes spp. tick vectors of Lyme disease-causing Borrelia spirochetes. Analysis indicates that Borrelia burgdorferi sensu lato species associated with Lyme disease are found mainly in ticks of the Ixodes ricinus ...
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